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Articles by M.M. Khorshed
Total Records ( 3 ) for M.M. Khorshed
  H.A. Murad , A.M. Abd El Tawab , A.M. Kholif , S.A. Abo El-Nor , O.H. Matloup , M.M. Khorshed and H.M. El-Sayed
  Tannase production by Aspergillus niger was evaluated under solid-state fermentation and submerged fermentation cultures. The optimum conditions for maximum enzyme production including deferent inoculum ratios, incubation periods, initial pH values, nitrogen and carbon sources were investigated. A. niger was grown as stand cultures in 250 mL conical flasks containing tannic acid powder medium. The maximum production of tannase by A. niger was achieved at inoculum ratio of 2% (v/v), 96 h of incubation period, initial pH 5.0, yeast extract as a nitrogen sources at a concentration of 0.33 g N L-1 and palm kernel powder (PKP) as a carbon source at a concentration of 25% (w/v). PKP was found to be the best carbon source supporting production of 931.27 U L-1 min-1 compared with 6.25 U L-1 min-1 for wheat straw.
  A.M. Abd El Tawab , O.H. Matloup , A.M. Kholif , S.A.H. Abo El-Nor , H.A. Murad , H.M. El-Sayed and M.M. Khorshed
  Two experiments were carried out to evaluate the effects of laboratory produced tannase enzyme (Tanozym) to diet including Palm Kernel Powder (PKP) on in vitro dry matter and organic matter disappearance (IVDMD and IVOMD) and in vivo nutrients digestibility, nutritive values, milk production and composition by lactating Baladi goats. In vitro experiment, IVDMD and IVOMD, were determined for control diets (60% CFM and 40% Berseem hay); (T1) control diet plus different levels of polyethylene glycol (PEG), being 10, 15 and 20 g kg-1 DM and (T2) control diet plus different levels of Tanozym (3.9, 5.85 and 7.8 U kg-1 DM). The maximum IVDMD and IVOMD values were observed with 5.85 U kg-1 DM for Tanozym and 20 g kg-1 DM for PEG compared to control, however there was no significant (p<0.05) difference between 15 and 20 g kg-1 DM. The in vivo experiment was carried out on nine lactating Baladi goats after 7 days of parturition where animals were divided into three groups, three animals each, using 3x3 Latin square design. The first group fed control diet (60% CFM and 40% Berseem hay), the second group fed T1 (control diet plus 15 g kg-1, DM), the third group fed T2 (control diet plus 5.85 U kg-1, DM). Tanozym supplementation significantly (p<0.05) increased nutrients digestibility, nutritive values, ruminal Total Volatile Fatty Acids (TVFA’s) but insignificant (p<0.05) increased ammonia nitrogen (NH3 N). Lower significant (p<0.05) values of rumen pH were recorded for treated groups compared with the control. Blood serum of animals fed Tanozym and PEG had higher values of total protein, albumin, globulin, total lipids, urea and glucose but lower values of AST and ALT compared with those of control. Daily milk yield, SNF, lactose and ash yield were significantly (p<0.05) increased with Tanozym compared control group. While, there are no significant (p>0.05) differences among groups for fat corrected milk 4%, total solids, fat and total protein yield.
  A.A. Aboamer , M.S.A. Khattab , S.A.H. Abo El-Nor , H.M. Saleh , A.M. Kholif , I.M. Khattab , M.M. Khorshed and H.M. El-Sayed
  Background: Synchronization the rate of which dry matter and crude protein degraded at the rumen significantly affects the synthesis of microbial protein and thus the efficiency of feed utilization. During previous study a new software application "Lacto-sheep" had been developed to facilitate the formulation of the least cost ration considered this important factor. So that formulation of a synchronous least cost ration is possible and not difficult. This study was carried out to investigate the advantages of feeding synchronous least cost ration as an alternative to the traditional least cost ration on nutrients digestibility and milk production and its constituents. Materials and Methods: The kinetics of dry matter and crude protein disappearance and approximate analysis were determined for each feed ingredients used. Then, using our feed formulation software "Lacto-Sheep" two diets; traditional least cost and synchronous least cost ration were formulated and fed to two groups of multiparous lactating Barki ewes (5 ewes each), suckling single with an average body weight (35.10±2.50 kg) using completely random design. Experiment started 2 weeks after lambing and lasted 60 days. Results: The synchronous least cost ration contained variety of protein sources compared with the least cost ration, which did not contain any rich source of protein (soya bean or cotton seed meal) that’s because the cost of feed is the main limiting factor regardless optimizing feed utilization. Digestion coefficient of crude protein was slightly improved in group fed synchronous least cost ration. In addition, milk production and its components were also slightly higher. However, the differences were not significant. Conclusion: Based on the results obtained from this study, synchronous least cost ration seemed to be more practically appropriate ration for feeding lactating Barki ewes.
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