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Articles by M.M. Hussein
Total Records ( 5 ) for M.M. Hussein
  Abd El-baky , H. Hanaa , Mohamed A. Amal and M.M. Hussein
  The changes that take place in the leaf apoplastic antioxidant enzymes system, protein and isoenzyme pattern in response to salts stress was studied in three onion (Allium ceba L.) cultivars, named Behary Red, Giza 6 and Giza 20. The constitutive level of lipid peroxidation and glutathione content (GSH), enzymes activity of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) as well as the protein and isoenzymes pattern were performed in the leaf of control (tap water, 200 ppm salts) and three concentrations of salt (2000, 4000 and 6000 ppm salts prepared from seawater (28000 ppm salts) stressed plants, 45 days after completing the stress treatments. The salt stress increased the rates of lipid peroxidation (as indicated by increasing the malonaldhyde (MDA) contents) and their effect was increased gradually by increasing salts levels. The increasing values in MDA contents in salt stress plants treated at 2000, 4000 and 6000 ppm salts levels were 121.9, 248.6 and 290.3% in Behary Red cv. and 126.4, 260.0 and 396.2% in Giza 20 cv., respectively of the control level (100%). Generally, salts stress induced significant increase in the activities of the antioxidative enzymes CAT, POD and SOD in onion cvs., accompanied by increased in glutathione level. The GSH level was increased more in Behary Red cv. and Giza 20 cv., than in Giza 6 cv., at 6000 ppm salts. Their increasing values were 203.7, 283.3 and 242.86%, respectively compared to the control level (100%). The leaf protein profile obtained by SDS-PAGE was diagnostic of characteristics different onion cultivars exposed to salts stress which, three distinct new protein bands with molecular weigh 50, 66 and 80 kDa were produced in Giza 6, Giza 20 and Behary Red onion cultivars, respectively. Also, electrophoretic bands of POD and CAT isoenzyme of different onion cultiver were differ in numbers and relative concentration due to salts stress. This finding suggested that Behary onion cultiver is better protected against lipid peroxidation process, inherently and under salts stress, than Giza 6 and Giza 20 onion cultivers. Also, the new protein bands and the characteristic CAT and POD isoenzymes banding patterns could be used as a biochemical marker for selection of salt tolerance onion plants when grown under salts stress.
  M.M. Hussein , Sawsan Y. El-Faham , Elham Z. Abd El-Moti and Nesreen H. Abou-Baker
  Background: Application of antioxidant materials like ascorbic acid to alleviate salinity stress and promote jojoba growth is a high effectiveness target, whereas, jojoba is an attractive industrial and biofuel crop. Materials and Methods: A greenhouse experiment was conducted in the National Research Centre at Dokki, Giza, Egypt to evaluate the effect of different salt stress degrees on the growth, photosynthetic pigments (chlorophyll and carotenoids) and mineral status characters of jojoba plant which sprayed by ascorbic acid. The treatments were as follows: (1) Irrigation by two concentrations of diluted seawater (S1 = 2500 and S2 = 5000 ppm), the control treatment irrigated with tap water (TW = 250 ppm), (2) Spraying ascorbic acid with two concentrations (As1 = 100 and As2 = 200 ppm), the control plants received the same quantity of distilled water (C = distilled water). Results: Irrigation with seawater negatively affected growth parameters in spites of that the differences not great enough to reach the significant levels. The high salinity level used sharply decreased chlorophyll a, chlorophyll b, carotenoids and total chlorophyll concentrations in comparable with that irrigated regularly with fresh water. Ascorbic acid application increased stem, leaves, shoot and seed protein, compared to control (foliar with distilled water), whereas, As2 was super than As1. Carbohydrate and carbohydrate/protein ratio also were increased with ascorbic acid application but As1 was the superior. Conclusion: Generally, it can be used diluted seawater in irrigation of jojoba plant with spraying ascorbic acid to alleviate the harmful effect of salinity.
  Essam S. Soliman , Mohamed A.A. Sobeih , Z.H. Ahmad , M.M. Hussein , H. Abdel-Latiff and A.A. Moneim
  A total of 1664 environmental samples (litter, water, swabs and air) were collected from commercial broiler farms located in Ismailia and Zagazig Governorates, Egypt. The bacterial and Fungal isolates that were identified included: Citrobacter Sp, E. coli, Klebsiella oxytoca, Proteus vulgaris, Pseudomonas aureuginosa, Salmonella Sp, Shigella Sp, Staphylococcus aureus, Streptococcus fecalis and Streptococcus pneumonie, Yeast Sp, Pencillium Sp, Aspergillus niger, Aspegillus flavus, Aspergillus nidulans, Mucour and Candida albicans Prevalence and frequencies of the microorganisms were calculated to detect the most predominant microorganisms. Litter samples showed that Pseudomonas areuginosa (24%) and Yeast Sp (37.5%) predominated in closed houses; Klebsiella oxytoca (12%) and Penicillium Sp (28.57%) predominated in open houses in winter, Klebsiella oxytoca (33.33%) and Aspergillus nidulans (22.73%) predominated in closed houses; E. coli (50%) and Penicillium Sp (21.40%) in open houses in spring, Shigella Sp (34.5%); (47.62%) and Aspergillus niger (26.92%); (8.69%) predominated in closed and open houses respectively in summer, Klebsiella oxytoca (55.56%) and Aspergillus niger (12%) predominated in closed houses; Klebsiella oxytoca (41.67%) and Candida albicans (59.1%) predominated in open houses in autumn. Water samples showed that E. coli (39.47%) and Candida albicans (50%) predominated in closed houses; E. coli (60.97%) and Penicillium Sp (60%) predominated in open houses in winter, E. coli (67.57%) and Penicillium Sp (37.5%) predominated in closed houses; E. coli (89.29%) and Aspergillus nidulans (15.83%) predominated in open houses in spring, Shigella Sp (42.55%) and Penicillium Sp (37.5%) predominated in closed houses; Shigella Sp (36.67%) and Yeast Sp (66.6%) predominated in open houses in summer, Klebsiella oxytoca (36.59%) and Candida albicans (33.3%) predominated in closed houses; Klebsiella oxytoca (47.22%) and Candida albicans (47.62%) predominated in open houses in autumn. Swab samples showed that Pseudomonas areuginosa (62.5%) and Penicillium Sp (29.41%) predominated in closed houses; Pseudomonas aureuginosa (47.06%) and Candida albicans (17.64%) predominated in open houses in winter, Klebsiella oxytoca (36.49%) and Aspergillus flavus (43.48%) predominated in closed samples; E. coli (43.48%) and Aspergillus flavus (46.51%) predominated in open houses in spring, Klebsiella oxytoca (28.98%) and Penicillium Sp (34.48%) predominated in closed houses; E. coli (35.17%) and Aspergillus niger (35.14%) predominated in open houses in summer, Pseudomonas areuginosa (31.75%); (42.31%) and Canidada albicans (40.32%); (61.4%) predominated in closed and open houses respectively in autumn. Air samples showed that staphylococcus aureus (51.72%-45.45%, 52%-56.17%, 59.52-69.44 and 48.78-75%) was predominating in closed and open houses respectively in winter, spring, summer and autumn respectively, while the fungal growth showed that Aspergillus niger (66.6%) predominated in closed houses in winter, Aspergillus niger (100%) predominated in closed houses; Aspergillus favlus (100%) predominated in open houses in spring, Aspergillus niger (100%) predominated in closed houses in summer, Aspergillus niger (100%) predominated in closed and open houses in autumn.
  Essam S. Soliman , Mohamed A.A. Sobeih , Z.H. Ahmad , M.M. Hussein and H.A.A. Moneim
  Five disinfectants [TH4® (combination of quaternary ammonium compounds and gluteraldhyde), Microzal® (combination of quaternary ammonium compounds and gluteraldhyde), Incospect IC 22XA (combination of quaternary ammonium compounds, gluteraldhyde and formalin), Povidone Iodine® (iodophore) and Formalin® (commercial formaldehyde 37%) were tested in a laboratory trials against four bacterial isolates (Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa) at concentration of (~105) isolated during epidemiological surveillance. The trials were carried in the presence and absence of organic matter (dried yeast 5%) using MIC use-dilution test. Minutely samples were collected for the bacterial counts. In the absence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 5, 5, 10 and 5 min (p<0.0001) respectively, Microzal® and Incospect IC 22XA achieved the 100% killing efficacy against the four Micro-organisms after 5 min (p<0.0001), Povidone Iodine® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively and Formalin® achieved the 100% killing efficacy after 5, 5, 5 and 20 min (p<0.0001) respectively. In the presence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 10, 5, 20 and 20 min (p<0.0001) respectively, Microzal® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively, Incospect IC 22XA achieved the 100% killing efficacy after 5, 5, 10 and 5 min (p<0.0001) respectively, Povidone Iodine® achieved the 100% killing efficacy against the four micro-organisms after 30 min (p<0.0001) and Formalin® achieved the 100% killing efficacy after 30, 5, 20 and 20 min (p<0.0001) respectively. The results revealed that quaternary ammonium-gluteraldhyde combination (TH4®, Microzal® and Incospect IC 22XA) although they are not proven to be environmentally safe; they are the most powerful disinfectants because of the synergistic action of the quaternary ammonium and gluteraldhyde bases.
  M.M. Hussein , M.S. Gaballah and S.Y. El-Faham
  Grains of barley (Hordeum vulgare L.) Cv. Giza 124 were treated by benzyl adenine and grown under different salinity levels (2000, 4000 and 6000 ppm of diluted seawater) where the concentration of different amino acids were determined. Phenyl alanine, serine, valine and glutamic acids increased in grains of plants subjected to 2000 ppm salts and tended to decrease with the higher salt levels. Proline, isoleucine and tyrosine concentrations increased by increasing salt concentration in the irrigation water up to 4000 ppm and then lowered in grains of plants treated with 6000 ppm salt solution. Threonine, serine, isoleucine, leucine, tyrosine and glutamic acids increased, however, proline and phenyl alanine concentration were progressively increased by spraying benzyl adenine at the rate of 50 ppm. On the other hand arginine, lysine, histidine and aspartic acids showed a significant decrease. Benzyl adenine application led to decrease in the concentration of all amino acids in grains irrigated by saline water 2000 ppm salts and significant increase at 6000 ppm compared to the control (tap water).
 
 
 
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