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Articles by M.M. Hassan
Total Records ( 2 ) for M.M. Hassan
  Elsayed B. Belal , S.M.H. Kamel and M.M. Hassan
  The present investigation was carried out to produce of antimicrobial metabolites by Bacillus subtilis during two successive seasons 2011/2012. One bacterial strain was isolated from healthy leaves of infected cucumber plants with Pseudoperonospora cubensis or Sphaerotheca fuliginea. Spraying of cucumber plants was done with bioagent (108 CFU mL-1) under greenhouse conditions as protective treatment comparing with fungicides. The obtained results exhibited that all treatments reduced the disease severity comparing with control. The microbial bioagent was identified by using 16S rDNA sequencing technique as Bacillus subtilis. The maximum accumulation of metabolites occurred at the stationary phase. Metabolites accumulation coincided with increase in the specific growth rate and reduction of disease severity. Maximum activity of metabolites was at pH 7 and 30°C. Mode of metabolites action on (sporangiophore and sporaniga) of P. cubensis and (conidiophore and conidia) of S. fuliginea by examination of Scanning Electron Microscope and their identification by GC-MS was investigated. It was observed that collapse in sporangiophore, sporangia and conidia. These compounds mostly included fatty acids known as Hexadecanoic acid, n-Hexadecanoic acid, Octadecanoic acid, 8-Octadecanoic acid, 9-Octadecanoic acid, Pentadecanoic acid, Heptadecanoic acid, dodecatrienoic acid, Nonanoic acid and Decanoic acid. Sprayed plants recorded best results for peroxidase and polyphenol oxidase enzymes activity compared with unsprayed one. Antibiotics exhibited also antagonistic activity against of Staphylococcus aureus by inhibition zone formation. In conclusion, bioagent can be use as an alternative and safe method to fungicides in controlling downy and powdery mildew diseases of cucumber and inhibition of Staphylococcus aureus as pathogenic bacteria.
  A.I. Fahmi , H.H. Nagaty , R.A. Eissa and M.M. Hassan
  The adverse effects of sea water salinity on number of nodules, nitrogen content, nitroginase activity, Chlorophyll a and b content, proline accumulation and protein pattern of faba bean plants (Vicia faba commercial cultivar Nubaria 1) were investigated. Faba bean plants were irrigated with sea water at 20, 25, 30, 40 and 50% concentrations and inoculated with rhizobial isolate ARC307 or with gamma rays treated isolates namely; ARC1, ARC2, ARC3, ARC4, ARC5, ARC6 and ARC7. Nodules number, nitrogen content, nitroginase activity and chlorophyll a and b content parameters were decreased by increasing sea water salinity with all used isolates, while proline accumulation parameter increased. At the same time, ARC2 isolate showed the highest values for these parameters above all isolates including the parental isolate ARC307 at all studied concentrations except for proline accumulation parameter, it was the least. Therefore, ARC2 considered as a promising isolate for salt tolerance. Salinity enhanced the occurrence of particular novel proteins in faba bean plants infected with ARC2 isolate.
 
 
 
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