Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by M.M. Ekperigin
Total Records ( 6 ) for M.M. Ekperigin
  O.A. Oseni and M.M. Ekperigin
  Calotropis procera (Sodom apple) or Bomubomu in Yoruba Language has been used locally in the Northern part of Nigerian and the Fulani set-ups in African settings as curdling agent in the production of ‘warankasi’ (a local soft cheese) when using cow milk; this plant is also known for its medicinal importance like antidote for snake bite, sinus fistula, rheumatism, mumps, burn injuries, body pain and treatment of jaundice in India and some parts of Africa. However, this present study was undertaken to characterize the milk clotting and proteolytic enzymes in the plant with the aim of establishing the optimal conditions of the enzymes to be used for cheese and allied milk products manufacturing and protein hydrolytic processes of medicinal importance. The results obtained from this study showed that the enzyme activities were evidenced in all the plant parts extract preparations except the root that had only proteolytic activity without noticeable milk-clotting activity for the period of one hour of investigation. The optimal pH for proteolytic and milk clotting enzyme activities were observed to be 4.0 for proteolytic and 5.0 for milk clotting enzyme activities in the crude extracts using casein and powdered milk as substrates, respectively while the temperature optima for the two enzyme activities were observed to be 70°C for milk clotting and 60°C for proteolytic. Calcium ion and cysteine were found to activate the two enzymes, with maximum activation at 800 mM Ca2+ for proteolytic activity and 10 mM for milk clotting activity while cysteine showed maximum activation at concentration of 6.0 mM for the two enzymes as Pb2+ ions caused inhibition of both enzymes at the very least concentration. The results of analyzes from this work showed how the proteolytic and milk clotting enzyme activities of the plant latex could be enhanced for protein hydrolytic processes of medicinal importance and industrial production of cheese and allied milk products.
  A. Onasanya , M.M. Ekperigin , Y. Sere , F.E. Nwilene , J.O. Ajele and G. Oboh
  Isozyme fingerprinting and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) isolates, causing rice Bacterial Leaf Blight (BLB) disease in West Africa, was carried out. Of 13 enzyme systems screened, Glucose 6-phosphate dehydrogenase (G6PH) showed adequate resolution and enzyme activity. Thus total proteins from all the 30 isolates were then analyzed using G6PH. This enzyme system was potentially useful as they differentiate all the 30 Xoo isolates studied. The study revealed 40-96.7% polymorphism in G6PH loci within the Xoo enzyme profile. These polymorphic isozyme loci were used to construct phylogenetic relationship cluster dendrogram among the 30 Xoo isolates. All the 30 Xoo isolates were classified into two major genetic groups (Xoo-A and Xoo-B) with five subgroups. Xoo-A possibly covers 46% and Xoo-B 54% of BLB population across West Africa. This study suggests the emergence of subgroup genotypes possibly the result of mutations and interactions among isolates and strains that originally made up Xoo-A and Xoo-B genotypes. The isozyme fingerprint defined for each race of Xoo could be useful for epidemiological surveys, disease diagnoses and in the identification of new virulent strains, isolates and their origin. This information could be useful in rice breeding programs aiming at development of durable Xoo resistant rice cultivars to different rice ecologies and localities in West Africa.
  A. Onasanya , A. Basso , E. Somado , E.R. Gasore , F.E. Nwilene , I. Ingelbrecht , J. Lamo , K. Wydra , M.M. Ekperigin , M. Langa , O. Oyelakin , Y. Sere , S. Winter and R.O. Onasanya
 

Case No: 26082013

This article has been withdrawn due to technical issue.

  A. Onasanya , M.M. Ekperigin , Y. Sere and J.O. Ajele
  Genetic diversityof 30 Xanthomonas oryzae pv. oryzae (Xoo) isolates, causing rice bacterial leaf blight disease in West Africa, was carried out using isozyme PAGE analysis. Of 13 enzyme systems evaluated, SKDH, EST and G6PH showed adequate resolution, enzyme activity and polymorphism and were used to analyze the total proteins from all the 30 isolates. The study revealed 23 isozyme loci in which SKDH produced 33.3-93.3% polymorphism, EST and G6PH equally gave 40-96.7% polymorphism within the Xoo isolates enzyme profile. These 23 isozyme loci were used to construct phylogenetic relationship cluster among 30 Xoo isolates, of which the Xoo isolates were classified into two major genetic groups (Xoo-A and Xoo-B) with two subgroups each (Xoo-A1 and Xoo-A2) and (Xoo-B1 and Xoo-B2). The 23 isozyme markers obtained clustered into 3 major groups (Gp-1, Gp-2 and Gp-3). Genetic study revealed that Gp-1 is genetically linked to the identification of Xoo-A1 genotype, Gp-2 to Xoo-A2 and Gp-3 characterized Xoo-B1 and Xoo-B2 genotypes. The distinct pattern of each isolate obtained suggests high level of genetic variation and frequent occurrence of mutants in Xoo isolates in different host cells. This information could be useful in rice breeding programs aiming at development of durable Xoo resistant rice cultivars to different rice ecologies and localities in West Africa.
  A. Onasanya , P. Kiepe , A. Basso , G. Nkima , F.E. Nwilene , I. Ingelbrecht , J. Lamo , M.M. Ekperigin , R.O. Onasanya , O. Oyelakin , S. Winter and Y. Sere
  Genomic DNA fingerprinting is a useful tool for effective and reliable identification and differentiation of Xanthomonas oryzae pv. oryzae (Xoo) pathogen from rice. The study aimed to conduct molecular characterization and DNA fingerprinting of 23 Xoo isolates from East Africa and two Xoo isolates from IRRI (Philippines) as control. PCR analysis was carryout on genomic DNA of 25 Xoo isolates using 6 Xoo specific primer pairs. Cluster analyses of genetic data obtained from 25 Xoo DNA fingerprints revealed two major genotypes (GrpA and GrpB) among the 25 Xoo isolates. GrpA has three subgroups (GrpA1; GrpA2; GrpA3) and GrpB (GrpB1; GrpB2; GrpB3). GrpA genotype consists of 20 Xoo isolates from Uganda, Rwanda and Philippines while GrpB genotype has 5 Xoo isolates from Rwanda. Some Xoo isolates were identical (PX-1, PX-2; UX621, RX2101; RX554, UX623, RX4113; UX211, UX213, UX214, RX4112, UX215). The emergence of subgroup genotypes could possibly be due to mutations and interactions among isolates and strains in host cells. Some Xoo isolates from Rwanda and Uganda were identical suggesting possible pathogen migration between these countries and long-term survival. Durable resistance rice cultivars would need to overcome both GrpA and GrpB Xoo genotypes in order to survive after their deployment into different rice ecologies in East Africa.
  A. Onasanya , A. Joseph , D.B. Olufolaji , M.M. Ekperigin , Y. Sere , F.E. Nwilene , P. Kiepe and R.O. Onasanya
  RYMV transmission by insect vectors is considered to fully represent how RYMV disease is spread under natural field conditions. The present study aimed to use Oxya hyla, Locris rubra and Chnootriba similes vectors after acquisition of the virus to determine RYMV movement and distribution in insect body and transmission to rice cultivars. RYMV susceptible BG 90-2 was sown in 5 L plastic pots each at 0.5, 1 and 1.5 m distance from test entries and seedlings were mechanically inoculated with a highly virulent RYMV Nigerian isolate 14 days after sowing. Seven days after inoculation of BG 90-2, test entries were sown in 5-litre plastic pots and same day Oxya hyla, Locris rubra and Chnootriba similes vectors were introduced into the screen house to feed on RYMV infected BG 90-2. RYMV content in Oxya hyla, Locris rubra and Chnootriba similes whole body was 71.8, 44.1 and 50 and head part was 42, 44.6 and 10.1%. RYMV incidence at 0.5, 1.0 and 1.5 m vector migration distance was 14.6, 16.0 and 19.0% for Oxya hyla, 31.3, 35.2 and 39.6% for Locris rubra and 13.7, 16.2 and 19.9% for Chnootriba similes. Cluster dendrogram revealed three groups (GrpA, GrpB, GrpC) of RYMV cultivar screening methods. GrpA was typical of Locris rubra, GrpB has mechanical and Oxya hyla while Chnootriba similes formed GrpC. The information reported in this study would help to better understand RYMV disease epidemic in farmers’ fields and to develop durable resistant rice varieties against the disease.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility