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Articles by M.M. Ali
Total Records ( 3 ) for M.M. Ali
  M.A. Abd El-Kader , M.M. Ali , N.M. El-Sammad and M.A. El-Shaer
  Non steroidal anti-inflammatory drugs are widely used and effective against inflammatory diseases. However, its clinical use is limited by its ulcerogenic effect. This study aimed to investigate the possible gastroprotective effect of alpha-lipoic acid on indomethacin-induced gastric ulcer in rats. Forty adult male Dawley rats were assigned into five groups of eight animals each : group 1 served as control, group 2 was treated intraperitioneally (i.p.) with alpha-lipoic acid (20 mg kg-1 b.w./day) for 7 successive days, group 3: Rats were treated i.p. with indomethacin (20 mg kg-1 b.w./day) for two days, group 4 were treated with indomethacin for 2 days followed by alpha-lipoic acid for 7 days, group 5: Animals were treated with alpha-lipoic acid for 3 days followed by indomethacin for 2 days then alpha-lipoic acid for other 4 days. Alteration of gastric mucosa were analysis. Gastric secretion volume, pH and gastric mucus secretion were determined. In addition, lipid peroxidation and the changes in the antioxidants: reduced glutathione, catalase and superoxide dismutase as markers for antioxidant activity and mucosal defense factors were evaluated. Administration of indomethacin per os induced gastric damage as evidenced by observed ulcers, histological changes, increased gastric secretion volume, decreased pH value and mucus secretion. Alpha lipoic acid were recovered rats from gastric mucosal lesions caused by indomethacin and protected the gastric mucosa covered the stomach wall from injury through decreasing gastric secretion volume, increasing pH value and mucus secretion. All parameters indicating the oxidative injury in gastric mucosa markedly reversed by alpha-lipoic acid treatment due to its potent antioxidant properties where it reduced the level of lipid peroxidation product malondialdehyde, alleviated increases in the catalase activity and ameliorated depressions in reduced glutathione level and the activity of superoxide dismutase caused by indomethacin injection. The results indicate that beneficial effect of alpha-lipoic acid in the protection and treatment of gastric ulcer which result from toxic effect of reactive oxygen species induced by indomethacin.
  R.F. Rahman , M.M. Ali , M.A. Salam , J. Ara , M.K. Ahsan and M.T. Haque
  Heterosis and combining ability studies of the isolated variable multivoltine races and developed bivoltine races with high yield capacity and their eighteen combinations were evaluated in two commercial rearing seasons in Bangladesh. Appropriate statistical methods were employed to interpret data on the hybrid performance, general and specific combining ability of the hybrids. Analysis of variance for combining ability revealed larger mean squares. Here high δ2gca2sca values for most of the characters reflected predominant additive gene action. Similarly, the variance of components of specific combining ability (δ2sca) of multivoltine×bivoltine were higher than those of general combining ability (δ2gca) for most of the characters in both the seasons indicating preponderance of non-additive gene action inheritance of all these traits. Among the combinations BSR-BN(B1)×BSRB-BB1 and BSR-3×BSRB-BB2 exhibited highly productive performance which could be recommended for commercial cocoon production in Bangladesh.
  M.M. Ali , D.A. Shokry , H.S. Zaghloul , L.A. Rashed and M.G. Nada
  Oral streptococci represent about 20% of the total oral bacteria, so if it is possible to detect the presence of oral specific bacteria from a forensic specimen by Polymerase chain reaction, this could be used to verify the presence of saliva. Aim of this study is detection of Streptococcus salivarius which is one of the most common streptococci in oral bacteria and Streptococcus mutans which is common in cases of dental caries in various body fluids and skin swabs and assessment of which one of both organisms is more reliable in saliva identification, cross sectional study on Egypt population. Negative control samples (15 samples) were taken from various body fluids (urine, semen) and skin swabs. Mock forensic samples (85 samples) included fresh saliva, saliva, cotton fabrics contaminated with saliva, cigarette butts, bitten apple and semen mixed with saliva samples). DNA extraction was done using DNeasy blood and tissue kit (Qiagen, Tokyo, Japan). Polymerase chain reaction was done for DNA amplification using Polymerase chain reaction master mix then gel electrophoresis was done for samples qualification. Control bacteria were S. salivarius and Streptococcus mutans. Streptococcus salivarius was detected in 83.5% of all saliva contained samples and S. mutans was detected in 67% of saliva contained samples. Both bacteria were not detected in other body fluids and skin swabs, so S. salivarius is more reliable in saliva identification as well as differentiating it from other body fluids. Polymerase chain reaction is valuable in detection of saliva by detecting S. salivarius.
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