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Articles by M.M. Adeyanju
Total Records ( 2 ) for M.M. Adeyanju
  M.M. Adeyanju , F.K. Agboola , B.O. Omafuvbe , O.H. Oyefuga and O.O. Adebawo
  The isolation and taxonomic characterization of Bacillus licheniformis isolated from cassava steep water and the purification and characterization of its extracellular amylase (α-1, 4-glucano-4-glucanohydrolase, EC 3. 2. 1. 1) were carried out in this study for the potential use of the enzyme for cassava starch hydrolysis for industrial purposes. The enzyme was purified by ion-exchange chromatography on DEAE-Cellulose and gel filtration on Bio-Gel P100 column. The specific activity of the purified enzyme was approximately 855 units per mg of protein (U mg-1). The enzyme is a large protein with apparent molecular weight determined by gel filtration on Bio-Gel P100 of greater than 100, 000 Daltons. The enzyme obeys sigmoidal kinetics with a kinetic constant (K1) for soluble starch of 1.097 ± 0.027% starch and a Vmax of 44.54 ± 1.79 U min-1. The optimum pH and temperature for enzyme activity were 7.5 and 90°C, respectively. The enzyme was stable for 45 min at 90°C. The enzyme was activated by Cd2+, Co2+, Mg2+ and Ni2+ while Fe3+ and Mn2+ moderately activated the enzyme and Zn2+, Ba2+, EDTA and acetamide were inhibitory. This amylase could be useful for the hydrolysis of soluble starch for the production of maltose.
  B.A. Salau , M.M. Adeyanju , K.T. Odufuwa and O. Osilesi
  Fruits and vegetables have been shown to contain vital components that moderate disease conditions. However, there is dearth of information of these effects in Africans diabetic subjects. Thus, we investigated the relative effect of fruits and vegetables on some selected heamorological factors associated with cardiovascular disease in diabetic subjects. Thirty diabetic subjects were selected at the cardiovascular clinic of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria. The subjects with mean Fasting Blood Glucose (FBG) level of 7.8±0.95 mmold L-1 were placed on 300 g of fruits and vegetables per day, while they continued their normal medication and food intake. A significant difference (p<0.05) was noticed between baseline and eight week values in all the parameters, Fibrinogen 262.75±2.64 to 250.57±3.98 mg dL-1, Blood viscosity, 5.96±0.21 to 4.82±0.23 m Pas; erythrocyte sedimentation rate 49.40±2.34 to 32.8±1.26 min h-1 and haematocrit 38.8±1.25 to 44.75±1.05%. At the eight week the fruits and vegetables were withdrawn for two weeks and the above parameters were reassessed (at the 10th week) which were compared with eight week values. Significant difference p<0.05 was noticed in E.S.R. 32.8±1.26 to 38.8±1.23. However no significant difference (p>0.05) was observed in haematocrit, 44.75±1.44 to 42.70±1.24, Fibrinogen, 254.5±3.98 to 250.57±3.16 mg dL-1. The results showed that regular intake of fruits and vegetables by diabetic subjects may reduce cardiovascular risk factors.
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