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Articles by M.M. Danish Daniel
Total Records ( 2 ) for M.M. Danish Daniel
  A.D. Talpur , A.J. Memon , M.I. Khan , M. Ikhwanuddin , M.M. Danish Daniel and A.B. Abol-Munafi
  Blue swimming crab, Portunus pelagicus has not anchored the roots in aquaculture due to non availability of commercial seed production. Letdown of seed production is owing to microbial infections. To combat with microbes, study was aimed to isolate and screen probiotics from the gut of female crab for larviculture. Based on characteristics of inhibitory activity against pathogenic V. harveyi, V. parahaemolyticus and P. piscicida, bile, acid, salt tolerances and survival in sea water, isolates were identified as L. plantarum, L. salivarius, L. rhamnosus W. confusa and W. cibaria and evaluated for probiotics. A new model small scale in vivo validation was developed for conformity of the isolates as probiotics for P. pelagicus larviculture. The LAB isolates were administrated as water additive at concentrations 102, 104 and 106 cfu mL-1 for one day and five days in vivo validation experiments and positive control was inoculated with same concentrations of V. harveyi while negative control employed with larvae and no inoculation. Highest larval survival achieved at concentration 106 cfu mL-1 and L. plantarum, L. salivarius and L. rhamnosus did show significant larval survival. W. confusa and W. cibaria did not demonstrate as probiotics. L. plantarum showed highest survival 49.45±4.80% and 54.44±6.74% in both inoculations, respectively and no survival was observed in five days+ve control. Water quality degradation was not evident but improvement in pH was noticed. Based on results of small-scale in vivo test three LAB probiotics, L. plantarum, L. salivarius and L. rhamnosus were selected for larviculture of P. pelagicus.
  A.D. Talpur , A.J. Memon , M.I. Khan , M. Ikhwanuddin , M.M. Danish Daniel and A.B. Abol-Munafi
  The cause of mass mortality of Portunus pelagicus larvae reared in a hatchery system was investigated. The gut content of 180 female crabs and egg specimen of 24 female were studied for pathogenic microbes. The gut of female crabs were harboring fish pathogenic bacteria includes Staphylococcus epidermidis, Vibrio harveyi, Vibrio parahaemolyticus, Micrococcus luteus and Pseudoalteromonas piscicida and eggs were found associated with fish pathogens include Vibrio harveyi, Micrococcus luteus and Pseudoalteromonas piscicida. A causative transmitting pathogen V. harveyi through the feces of adult female crab and responsible for heavy mortality during larval rearing was determined by examining samples associated with the gut, hatching tanks, eggs, larvae rearing tanks, live and dead larvae of P. pelagicus. All isolates were identified by 16S rRNA gene sequencing. Vibrio harveyi was the major pathogen associated with all sources brought under study. Larvae were found to harbor a higher number of bacteria than larvae rearing tank. Experimental challenges with various doses indicated that the V. harveyi isolates were highly pathogenic. Doses 105 cfu mL-1 produced upto 96.67% mortality and 106 cfu mL-1 resulted in 100% mortality within 24 h post challenge. The differences among various doses of pathogen were statistically significant (p<0.05). The presence of these pathogens in P. pelagicus beyond the consequence for larval rearing is of epidemiological and health significance to humans.
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