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Articles by M.M. Ahasan
Total Records ( 3 ) for M.M. Ahasan
  T. Ahamed , K.M. Hossain , M.M. Billah , K.M.D. Islam , M.M. Ahasan and M.E. Islam
  Newcastle disease virus (NDV) is the infectious agent of Newcastle disease in poultry. This virus can grow within different animal cells including primary cell culture and established cell line. In order to adapt NDV on African green monkey kidney (Vero) cell line, five consecutive passages were done. Eagle`s minimum essential medium (EMEM) with supplements was used for both culturing Vero cells and maintaining NDV on Vero cells. During first and second passage, wild NDV didn`t produce any clear evidence of cytopathic effect (CPE), but in third passage changes in the characteristics of cell monolayer were observed. During fourth and fifth passages, clear and consistent CPE were observed within 50 to 60 hours of infection. CPE was characterized by formation of syncytium, giant cell, dendritic-shaped cell and finally plaque. The titer of passage 5 (P5) virus was 10-3.9TCID50, whose purity was tested by serum neutralization test (SNT) and the result was 1.6 x 104 units/ml.
  M.M. Ahasan , K.M. Hossain and M.R. Islam
  This experiment was conducted to study the adaptation of IBDV on vero cell line. Suspected IBDV isolates were collected from the bursa of dead chicken of a particular flock. For adaptation, the anchorage-dependant monolayer of vero cell was first subcultured to form semi-confluent monolayer in eagle`s minimum essential medium (EMEM) with 5% fetal calf serum. This semi-confluent monolayer was then infected by local field IBDV isolates. The passage 1 (P1) viruses were harvested and used for the next passage. In this way, the viruses were given three serial passages on vero cell line where characteristic cytopathic effects (CPEs) were observed. During the first passage, no CPEs were found. The infectivity of IBDV on vero cells was observed by changes in the characteristics of vero cell monolayer. During the third passage, clear and consistent CPEs were observed. Vero cell monolayer was changed to form rounding cells. The P3 adapted viruses were confirmed to be IBDV by agar gel precipitation test. The P3 IBDV virus became well adapted to vero cell line.
  P. Shrestha , M.M. Ahasan , K.M.D. Islam , M.M. Billah , M.E. Islam , M. Mehedi , S. Mitra and M.R. Islam
  In the present experiment, Enzyme linked immunosorbent assay (ELISA) was applied on a total of 49 samples collected from 4 breeds of chicken (BV-300, Broiler Kasile, LBM and Hisex) at different age (day 1, day 5, day 10 and day 15) to determine the level of maternally derived antibody (MDA) against infectious bursal disease (IBD). All the chickens were the progeny from the parentstock that had the history of vaccination. A total number of 10 broilers were used to determine the level of IBDV specific antibody in vaccinated and in non-vaccinated chickens following infection with field virus suspension. As these chickens attained the age of 14 days, 6 chickens were vaccinated with Gumboro D78 live vaccine while remaining 4 chickens were kept without vaccination. All the chickens were infected with field virus suspension on day 19 and blood samples collected on day 29 were subjected to ELISA. Slight variation in the antibody titer was observed among 4 breeds of chickens. An average antibody titer of 5320.79, 5877.15, 3676.24 and 5581.55 was found in day old BV-300, Broiler Kasile, LBM and Hisex respectively. Day old BV-300 contained high level of MDA (average of 5320.79) and the level gradually declined and persisted up to 15-20 days. Five days old, 10 days old and 15 days old BV-300 contained an average antibody titer of 3848.57, 2615.53 and 580.88, respectively. On day 29, there was a significant level of antibody (1489.50), much above minimum protection level, in vaccinated chicken whereas nil antibody level was observed in non-vaccinated chickens. Therefore, the chicks should be vaccinated at around day 14, at which time the antibody level reaches nearly to minimum protection level. Antibody level must be carefully monitored at proper interval of time in order to make the vaccination program more effective, to keep the chickens disease free, to increase the production and to prevent the economic loss.
 
 
 
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