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Articles by M.L. Gomes
Total Records ( 4 ) for M.L. Gomes
  A.R. Souza , A. Silva , T. Freitas , R. Freitas , B. Mazziotti , M.L. Gomes , G.F. Dir and M. Bernardo Filho
  Although biological effects of ultrasound must be avoided for safe diagnostic applications, the ultrasound ability to disrupt cell membranes has attracted interest as a method to facilitate drug and gene delivery. It is concerned that many natural or synthetic medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Over the last few decades, application of ultrasound has been attempted to enhance transdermal transport of several drugs, a method referred to as phonophoresis. It has been related that natural and synthetic products in folk or clinical medicine have been widely used by human beings due to its curative properties. However, sometimes the biological effects of these products are not fully known. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural and synthetic products are capable of reducing the efficiency of this radiolabeling. The purpose of this study was to investigate the effects of phonophoresis on the radiolabeling of blood elements with 99mTc in an in vivo study. The animals of the therapeutical group under anesthesia with ether, the rats had been submitted to the therapy with ultrasound of high frequency (0.8 to 1Mhz) in continuous way, in the dose of 0.5 W/cm , during 3 min, with perpendicular and circular movements of 2.5 cm of diameter 2 on the back during 6 days. It was used as half of contact between the transducer and the skin the proper gel (diclofenac) for ultrasound. After that, samples (0.5mL) of blood were incubated with stannous chloride (SnCl ) 2 and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that in the treated group was observed a reduce on the radiolabeling in IF-P (74.74%? 1.35 to 62.47.26%? 5.35). In the light of the results we suggest that the phonophoresis allows the attainment of higher local concentrations of diclofenac may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process of plasma proteins.
  M.L. Gomes , E.A.C. Lima , R.L. Jales , M. Castro Faria , M. Bernardo-Filho and G. Dire
  The use of natural products occurs around the world. The labeling of blood constituents with technetium-99m (99m Tc) has been influenced by natural extracts. We evaluated the influence of a chayotte(Sechium edule) extract on the labeling of blood elements with 99m Tc, in the structural conformation of DNA, in the biochemistry of blood and in the measurement of. blood pressure. The animals were treated with chayotte during 15 days and samples of blood were withdrawn. The samples were incubated with stannous chloride and with 99mTc. Plasma(P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble(SF) and insoluble fractions(IF) separated. There was a decrease in the radioactivity in IF-P(from 83.96 ± 4.28 to 53.26 ± 6.69). Samples of blood from the treated group were carried out with specific biochemistry kits and the blood biochemistry analysis compounds was done. It was analyzed the level of uric acid, albumin, cholesterol, creatinine, glucose, high density lipoprotein (HDL), globulin and trigliceridics. The gauging of the blood pressure of the animals was taken. Our results showed a reduction on the level of glucose (from 118.40 ± 10.69 to 97.20 ± 4.32) and globulin (3.52 ± 0.13 to 3.08 ± 0.19) as well as in the diastolic pressure (from 123.80 ± 9.12 to 84.40 ± 3.85). It was observed that the referred extract has induced lesions on the DNA molecule. The effect of chayotte extract probably, could be explained by the metabolization of the chayotte that could be capable to induce the generation of active metabolites with oxidant properties.
  M.L. Gomes , E.A.C. Lima , S.M.S. Souza , L.C. Bernardo , M.E. Penas , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural or synthetic drugs can alter the bioavailability of radiopharmaceuticals used in diagnostic imaging in nuclear medicine. The knowledge of such altered bioavailability is important in making diagnostic of diseases and to try to understand biological effect of drugs in specific organs. The use of natural products has been increasing in the world. Echinacea is a natural product widely used for the treatment of respiratory tract infections. The aim of this study was to evaluate the effect of the echinacea on the bioavailability of the radiopharmaceutical 99mTc-sodium pertechnetate (Na99mTcO4) and on the mass of organs to try to verify possible undesirable actions of the studied natural product. An extract of Echinacea was daily administered by intragastric via into Wistar rats during 10 days. Na99mTcO4 (3.7MBq) was administered by ocular plexus via and 10 minutes after the animals were sacrificed. Blood and various organs were removed, their mass determined, their radioactivity counted in a well counter and the percentages of the injected dose per gram of organ (%ID/g) calculated. The results showed that in the treated animals: (i) the mass of the organs was not modified; (ii) the %ID/g decreased in lungs. The effect of echinacea on the Na99mTcO4 bioavailability in the lung was statistically significant (Student test, p<0.05) and it could be explained by the metabolization or therapeutic action of this herb in this organ.
  G.F. Dire , E.A.C. Lima , M.L. Gomes , S. Moreno , M.V.C. Faria , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. It is concerned that many natural medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radio labeling. The aim of this work was to assess the oxidant or antioxidant action of the agents presents in the extract of Sechium edule verifying its effects on the labeling of blood elements with 99mTc and on the inhibition of the activity of acetyl cholinesterase (AchE). A freshly extract of Sechium edule was administered to Wistar rats during 15 and 60 days. After that, samples (0.5ml) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the referred extract was able to reduce the radio labeling in BC to 15 days of treatment (from 98.77%± 0.45 to 90.35%± 5.04) to 60 days of treatment (from 98.77%± 0.45 to 53.53%± 0.91), in IF-BC (from 90.36%± 2.34 to 21.20%± 2.36) and in IF-P to 15 days of treatment (77.20%± 2.19 to 53.26%± 6.69) to 60 days of treatment (77.20%± 2.19 to 11.01%±3.22). The analysis of toxicology was performed by the determination of acetyl cholinesterase activity. In the light of the results the referred extract has not shown inhibitory action due to the activity of the enzyme. In the light of the results obtained we suggest that the referred extract may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process.
 
 
 
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