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Articles by M.K. Tripathi
Total Records ( 2 ) for M.K. Tripathi
  M. Kumar , B. Sharma , A. Kumar , H.P. Lal , V. Kumar and M.K. Tripathi
  Toxocara canis is a very important gastrointestinal nematode affecting canines with considerable public health importance. This study was conducted to find out the prevalence and corresponding haemato-biochemical changes in dogs infested with T. canis and to determine its zoonotic implication to dog owners. A total of 121 dogs were screened from October 2008 to May 2009 by direct smear and Mc-master technique, to determine correlation between overall prevalence of T. canis infestation with respect to sex, age, breed, size and season-wise infestation. Haemato-biochemical profile was performed in 24 infested dogs, randomly selected to evaluate changes in Hb, PCV, TEC, TLC, DLC count, serum protein, serum glucose and serum enzymes. The overall prevalence was found to be 28.93%. The prevalence was not influenced by sex but non-descript breeds had significantly higher rates. Pups were more infested than adults and the disease was more prevalent in winters. Dogs having active infection with T. canis infestation showed anemia, leucocytosis and significant eosinophilia (p<0.05). A significant decrease (p<0.05) was observed in serum protein and glucose whereas highly significant increase (p<0.01) was found for both serum enzymes (SGOT and SGPT). Very few dog owners (4.13%) were aware about potential public health significance of the parasite. Survey revealed that unaware owners who belonged to lower/middle/upper middle class, did not maintain hygiene and scheduled deworming and always remain at high zoonotic risk. Being zoonotic, the parasite poses a significant danger to humans mainly children who remain in their vicinity. Thus immediate action needs to be taken to control this parasite and to increase awareness among the dog-owners about the zoonoses being spread by the companion animals.
  M.K. Tripathi , S. Tiwari and U.K. Khare
  Disease tolerant/resistant onion (Allium cepa L. cv ADR and ALR) cell lines were selected against purple blotch disease caused by Alternaria porri. For this purpose callus and cell suspension cultures derived from mature embryos were exposed to purified toxic culture filtrate produced by the fungus supplemented with MS culture medium. Two selection methods were used: a continuous method in which four cycles of selection were performed on toxic medium whereas during discontinuous method, a pause was given after the second and third cycle of selection using non-toxic medium. Almost 4700 calli obtained from mature embryo and 8300 cell clumps from cell suspension cultures of two onion cultivars were exposed to media with phytotoxin for selection. The discontinuous method proved to be superior as it allowed the calli to regain their regeneration capability. Continuous exposure with toxic culture filtrate resulted up to 78% mortality. In vivo pathogenesity test of regenerated plants from the surviving tolerant/resistant cell lines revealed non-sensitive against pathogen toxin. A total 4 lines of ADR and 2 of ALR were documented resistant/tolerant amongst an array of putative resistant/tolerant lines during S1 generation.
 
 
 
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