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Articles by M.K. Thori
Total Records ( 2 ) for M.K. Thori
  N. Kataria , Abhimanu , S. Arora , A. Sharma , R. Maan , S. Soren , A.K. Kataria , N. Mohammad , P.K. Pilania , L.N. Sankhala , M.K. Thori , J.S. Gaur and A. Meena
  A study was carried out in male and female calves of Rathi breed belonging to arid tracts from India to find out status of free radical scavenging enzymes during hot ambience. Blood samples were collected during moderate and hot ambiences to harvest sera. Maximum ambient temperature ranged from 44.5-46.5°C during hot ambience. All calves were managed in similar conditions and were apparently healthy. Free radical scavenging enzymes included Superoxide Dismutase (SOD), Glutathione Reductase (GR) and Catalase (CAT). The moderate (control) mean values of serum SOD, GR and CAT were 173.00±1.43, 11.00±0.09 and 74.80±1.00 kU L-1, respectively irrespective of gender and age. Similar pattern of significant change (p≤0.05) was observed by all the three enzymes in heat stressed calves being higher during hot ambience. The mean values were 2.67, 2 and 1.41 times higher, respectively in heat stressed calves during hot ambience. The sex and age effects were significant (p≤0.05) in moderate and hot ambiences for all the three enzymes. The mean values were significantly (p≤0.05) higher in male animals than female animals. In each ambience the age effect showed a significant (p≤0.05) increase in the mean values being highest in the calves of 6 months to 1 year of age. Results clearly indicated that status of free radical enzymes changed in heat stressed calves probably to scavenge higher number of free radicals formed during hot ambience. All the three enzymes used are important biomarkers of oxidative stress. Present study provided data which can be used as reference values to assess the extent of oxidative stress. This will help in timely protection of calves from ensuing health disorders.
  P.K. Pilania , S. Solanki , N. Mohammed , S. Asopa , R. Maan , A. Joshi , L.N. Sankhala , M. Mathur , M.K. Thori , J.S. Gaur , A. Meena and N. Kataria
  An investigation was carried out to assess oxidative stress in goats of Marwari breed affected from gastrointestinal parasitism and pneumonia belonging to farmers’ stock of arid tract of Rajasthan State, India. The animals were grouped into healthy and affected. All the blood samples were collected in sterile tubes with anticoagulants for erythrocyte separation and determination of erythrocytic Catalase (CAT) and Superoxide Dismutase (SOD) as biomarkers of oxidative stress. Activities of both the enzymes were significantly (p≤0.05) higher in affected animals in comparison to healthy lot. Erythrocytic catalase activity was 2.37 times higher in goats having gastrointestinal parasitism and 3.06 times higher in goats affected with pneumonia. Erythrocytic superoxide dismutase activity was 4.77 times higher in goats having gastrointestinal parasitism and 6.40 times higher in goats affected with pneumonia. It was observed that goats affected with pneumonia showed higher magnitude of enzyme activities in comparison to those having gastrointestinal parasites. Magnitude of change was greater in SOD activities than CAT activities. It can be concluded that gastrointestinal parasites and pneumonia served to produce oxidative stress in the goat. Catalase and superoxide dismutase are considered as potent biomarkers of oxidative stress. They are also named as antioxidant enzymes. Increased activities of both these enzymes signified the presence of oxidative stress in order to combat excessive production of free radicals. Antioxidant supplementation is recommended in cases affected with conditions like parasites and pneumonia to protect the animals from oxidative stress and ensuing damage to cellular machinery.
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