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| Articles
by
M.K. Gharib Naseri |
Total Records (
3 ) for
M.K. Gharib Naseri |
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A.A. Moazedi
,
N. Dabir
,
M.K. Gharib Naseri
and
M.R. Zadkarami
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The aim of present study was to investigate the role of opioid and α-adrenergic receptors in antispasmodic activity of Ruta chalepensis (L.) hydroalcoholic extract (RCHE) in rat ileum contractions induced by KCl. A piece of ileum (2 cm) was dissected out from male Wistar rats and mounted in an organ bath containing air bubbled Tyrode solution (37°C). Isotonic Transducer is exceptionally sensitive and stable and connected to Harvard Universal Oscillograph (UK). The tissue was under 1 g initial tension and contractions were recorded isotonically. The RCHE was prepared by macerated with alcohol (70%). Ileum was contracted by KCl (60 mM) and cumulative concentrations of RCHE were applied (0.01-0.07 mg mL-1) before and after tissue incubation (30 min) with naloxon (1 μM) or phentolamine (1 μM). RCHE reduced KCl-induced contraction in a concentration dependent manner (p<0.001). However, tissue incubation with naloxone and phentolamine attenuated the spasmolytic effect of RCHE (p<0.001). We conclude that in the antispasmodic activity of RCHE, at least in part, the opioid and α -adrenergic receptors are involved. |
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A.A. Moazedi
,
N. Dabir
,
M.K. Gharib Naseri
and
M.R. Zadkarami
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In this study, the effect of Ruta chalepensis
on rat’s ileum contraction has been investigated because the hydroalcoholic
extract of the leave of Ruta chalepensis, grown in Iran were investigated
for pharmacological properties. Ruta chalepensis (Rue) is annual
herb is used in the traditional medicine in Iran for intestinal disorder.
In this study the effect of hydroalcoholic extract of Rue on isolated
rat’s ileum contraction was investigated by KCl (60 mM). A segment of
ileum was removed and suspended in 10 mL organ bath containing aerated
Tyrode solution (37 °C, pH = 7.4).The responses were determined and
recorded on isotonic transducer and Harvard Universal Oscillograph. The
result demonstrate that the extract significantly reduced the ileum contraction
induced by KCl (60 mM) in a dose dependent manner (n = 7, p<0.001).
Also, adding extract before influence of KCl, cause reduce effect of KCl
(n = 7, p<0.001). In another study, in high potassium Ca2+-free
tyrode solution, cumulative concentrations of CaCl2, induced
ileal contraction, however, the extract reduced these contractions in
a dose dependently (n = 7, p<0.001). Therefore, the results suggest
that relaxatory effect of rue on the ileum may be due to blocking of voltage
gated calcium channels. |
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F. Hosseini
,
M.K. Gharib Naseri
,
M. Badavi
,
M.A. Ghaffari
,
H. Shahbazian
and
I. Rashidi
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Renal ischemia/reperfusion injury is a major cause of acute renal failure. The production of free radicals and reactive oxygen species are important factors contributing to ischemia/reperfusion injury. Thus, scavenging of the excess free radicals can be an important therapeutic approach. The present study examined the protective effect of beta carotene against renal ischemia/reperfusion injury in rat. Male adult Wistar rats (250-300 g) were exposed to 45 min of renal ischemia followed by 4 h of reperfusion. Beta carotene (10, 30 and 100 mg kg-1) or vehicle was administered for 5 days prior to ischemia. Renal function was assessed by plasma and urinary analysis. Present results showed that ischemia/reperfusion injury increased (p<0.05-p<0.001) serum urea and creatinine levels, as well as urinary excretion of protein and calcium and fractional excretion of sodium, while decreased glomerular filtration rate and potassium excretion. However, alterations in these biochemical indices due to ischemia/reperfusion injury were attenuated by beta carotene pretreatment (p<0.05-p<0.001), although not by all doses. Since, beta carotene administration improved renal function, it seems that beta carotene protects renal tissue against ischemia/reperfusion-induced oxidative damage. |
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