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Articles by M.J. Mahdavi
Total Records ( 5 ) for M.J. Mahdavi
  Khalesi, E. , G.H. Shahidi Bonjar , S. Aghighi , M.J. Mahdavi and A. Ayatollahi Moosavi
  Emergence of Azole-resistant Candida albicans is a growing problem worldwide. Searching for new principles is a global need with high priority. With this aim, in vitro anticandidal activity of pure cultures of soil Actinomycetes isolated from Kerman Province were screened against a registered isolate of C. albicans (PTCC No. 5027). In comparison with Clotrimazole, the drug of choice of the pathogen, from 50 tested Actinomycetes, crude sap of Streptomyces olivaceus strain 115 showed in vitro antagonistic anti-yeast effect on the pathogen. The tests performed aseptically on lawn cultures of the pathogen in well diffusion bioassay system. Inhibitory zones represented complete suppression of C. albicans. The nature and further biological activity of the active principle are under investigation.
  F. Aram , G.H. Shahidi Bonjar , M.J. Mahdavi , S. Mansouri , P. Rashid Farrokhi , N. Bana Hosein Pour , M. Shekari , A. Ghasemi and S. Aghighi
  Streptomyces sp. are mostly soil-inhabitants and several species are responsible for important diseases in plants, S. scabies causal agent of Common Scab disease of potato and human S. somaliensis causal agent of Mycetoma. Methanolic extracts of leaves of Myrthus communis and seeds of Terminalia chebula, which had documented uses in Iranian herbal-medicine, were tested for anti-Streptomyces activity against S. scabies. For bioassays, concentration of 50 mg mL-1 of methanolic extracts prepared in dimethyl sulfoxide: methanol (v/v, 1/1) and tested in well diffusion method. Inhibitory zones (IZ) in contrast to controls were measured five days after inoculation at 29°C. Both plants showed high biological activity against S. scabies. Future goals include bioassays on human and further plant pathogenic Streptomycetes.
  F. Sharifi , G.H. Shahidi Bonjar , S. Shafii Bafti , B. Barkhordar , S. Aghighi and M.J. Mahdavi
  Streptomyces sp. are of the most attractive sources of biologically active compounds. In the recent decades, they have attracted high interests as biocontrol agents. In search of finding such principles, in vitro suppression of Alternaria solani was studied by use of Streptomyces antagonists. A. solani was sensitive to several species mainly Streptomyces plicatus, S. olivaceus and two unidentified actinomycetes. Activity versus time was monitored in S. olivaceus in solid and shaked cultures. Ongoing goals of this research include isolation, characterization and identification of the active metabolites. Future goals include identification of active genes for use in development of recombinant DNAs in transgenic hosts bearing elevated resistance to infections by Alternaria sp.
  G.H. Shahidi Bonjar , P. Rashid Farrokhi , Shafii Bafti , S. Aghighi , M.J. Mahdavi and A. Aghelizadeh
  In greenhouse cucurbits of Kerman Province, Fusarium oxysporum f.sp. melonis Schlecht, Emend (Snyder and Hansen) causes root rot and fusarium wilt. To investigate for new biofungicides, antagonistic activity of soil Actinomycetes isolates were assayed against the pathogen from which Streptomyces olivaceus strain 115 showed anti-fusarium activity both in vitro and in vivo experiments. The active strain was grown in aqueous media on rotary shakers to monitor activity versus time and prepare active dry crude for further biological and physical studies. Antifungal activity was of fungistatic type on the pathogen mycelia. From the results of our studies it is clear that usage of S. olivaceus strain 115 as a biofungistatic natural product applied as an amendment in greenhouse soil mix will lead to inhibition or reduction of the pathogen effects.
  G.H. Shahidi Bonjar , B. Barkhordar , N. Pakgohar , S. Aghighi , S. Biglary , P. Rashid Farrokhi , M. Aminaii , M.J. Mahdavi and A. Aghelizadeh
  Actinomycetes enhance soil fertility and have antagonistic activity against wide range of plant root-pathogens. These microorganisms were isolated from agricultural soils of Kerman as pure cultures. Phytophthora drechsleri Tucker, causes gummosis and root rot of pistachio trees worldwide. From 130 Actinomycetes isolates, 12 inhibited growth of the pathogen of pistachio gummosis in culture plates and four of the most active isolates exhibited biological control of the pathogen under greenhouse conditions. When plants were grown in sterile soil mix and treated both with Actinomycetes and the pathogen, the number of healthy plants increased dramatically and the symptoms on diseased plants were less severe in comparison with seedlings treated with the pathogen alone. From the collected data it was well conclusive that in greenhouse tests, soil applications of Actinomycetes controlled causal agent of root rot of pistachio seedlings. Antifungal activity was of fungicidal type on the pathogen mycelia. From the stand point of biotechnological goals, the results indicate that the active isolates can be investigated for use as biofertilizers, biofungicides and use in future development of recombinant DNA in pistachio trees bearing elevated resistance to gummosis. Field trials of the active isolates are under investigation.
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