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Articles by M.J. Ikenebomeh
Total Records ( 3 ) for M.J. Ikenebomeh
  C.N. Obi and M.J. Ikenebomeh
  Fresh milk sample collected from lactating cows was subjected to microbiological and nutritional analyses. It was fermented by indigenous microbes. The micro organisms involved were isolated at two-hourly intervals till fermentation was terminated at 12 h. The samples at intermediate stages of fermentation were also subjected to the same analyses. Titratable Acidity (TA) was carried out and the corresponding pH determined. The organisms isolated from the fresh and fermented milk samples included Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Bacillus cereus, Pseudomonas aeruginosa, Lactobacillus lactis, Saccharomyces cerevisiae and Geotricum candidum. The fresh milk sample has a microbial load of 2.1x10 6 cfu mL-1, pH of 6.68 and moisture content of 88% while the fermented product has a pH of 5.7, moisture content of 85%. The fresh milk also has a higher protein, lipid, cholesterol and Low-density Lipoprotein (LDL) values but lower High-density Lipoprotein (HDL), glucose and ash content than the fermented milk (Nono).
  I.S. Ogiehor and M.J. Ikenebomeh
  The antimicrobial effect of different concentrations (0.6, 0.4 and 0.2%) of sodium benzoate (SB) on the growth, survival and aflatoxin production potentials of Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus in packaged garri (2kg/pack) during storage at ambient temperature (30±2oC) was investigated. A decrease of 4.5, 2.5, 1.9; 4.5, 2.4, 1.8 and 4.6, 2.6 and 2.0 folds were recorded in the viable count of A. niger, A. flavus and A. fumigatus, for 0.6, 0.4 and 0.2% SB treated samples respectively. The effectiveness of SB against the various species of Aspergillius was in the order 0.6% > 0.4% > 0.2%. However 1.2 and 1.7 folds increase in the viable count of A. niger, A. flavus and A. fumigatus were recorded in the non treated samples (controls). No aflatoxin was detected in all the samples treated with different concentration of SB. However, aflatoxin was detected in the samples inoculated with Aspergillus flavus although unspecified and irregular florescence was noticed in other samples (controls).
  C.E. Oshoma and M.J. Ikenebomeh
  Growth of Aspergillus niger on rice bran medium in submerged fermentation was studied using standard methods for determinations of dry biomass, total crude protein and final pH values. It was observed that A. niger biomass cropped on rice bran medium supplemented with glucose, (NH4)2SO4, KH2PO4, MgSO4.7H2O and ZnSO4.7H2O designated as Glucose supplemented rice bran (GRB) medium was highest at 2.03±0.02g/l while the unsupplemented rice bran medium (RBM) has the lowest value of 0.97±0.00g/l. The effect of various nitrogen sources on the final biomass yield was compared. Ammonium sulphate [(NH4)2SO4] gave the highest biomass yield of 1.95±0.03g/l and sodium nitrate (NaNO3) had the lowest yield of 0.97±0.02g/l. Glucose and nitrogen supplements increased biomass yield.
 
 
 
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