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Articles by M.H. Awaad
Total Records ( 3 ) for M.H. Awaad
  Wafaa A. Abd El- Ghany , M.H. Awaad and S.R. Nagwa
  In this study, the efficacy of acidifiers (lactic and formic acids) and probiotic preparations containing Pediococcus acidilactici (P. acidilactici) and Saccharomyces boulardii (S. boulardii) in reducing of Campylobacter jejuni (C. jejuni) infection in broiler chickens was investigated. One hundred and three day old broiler chicks were used. Three birds were euthanized for C. jejuni re-isolation at day old. One hundred chicks were assigned into 4 equal groups each, 25 birds. Groups 1, 2 and 3 were fed on ration containing acidifiers, P. acidilactici and S. boulardii, respectively, from day old till 5 weeks old. Chicks of group 4 were fed on plain ration (control). At 2 weeks of age, each bird was orally challenged with C. jejuni. Groups were kept for 3 weeks recording signs and mortalities. Faecal swabs were collected at different intervals for C. jejuni shedding. Liver and intestine were collected weekly for C. jejuni re-isolation. At 5 weeks of age, all birds were subjected for post-mortem lesions scoring of C. jejuni. Results cleared more severe signs of greenish diarrhea in control than treated birds with no mortalities in all groups. Significant (p<0.05) reduction of C. jejuni shedding and re-isolation rates as well as lesions scoring was seen in groups treated with S. boulardii and P. acidilactici followed by acidifiers over control birds. In conclusion, both acidifiers and probiotic preparations greatly reduced and eliminated C. jejuni infection in broiler chickens.
  Wafaa A. Abd El-Ghany , M.H. Awaad , S.A. Nasef and A.F. Gaber
  This study was designed to examine the effect of using sodium butyrate encapsulated in palm fat in comparison with enrofloxacin on Salmonella Enteritidis (SE) infection in broiler chickens. Two hundred, day-old broiler chicks were allocated into 5 equal groups (n = 50). Group 1 was kept without challenge or treatment (blank control), Group 2 was fed on sodium butyrate, Group 3 was challenged and treated with enrofloxacin, Group 4 was challenged and treated with sodium butyrate and Group 5 was only challenged (positive control). Challenged groups were orally inoculated with 0.3 mL (1.5×108 SE/mL/chick) at the 2nd day of age. Enrofloxacin was given at the 3rd day of age in water (10 mg kg–1 b.wt.) for 5 successive days; however, sodium butyrate was added in doses of 1.0, 0.5 and 0.25 kg t–1 for starter, grower and finisher ration respectively from day till 5 weeks old. The results revealed no mortalities and decrease in the severity of signs and lesions in treated groups than positive control one. At the 4th week of age, sodium butyrate supplement gave significant (p≤0.05) improvement in body weight, weight gain and feed conversion than others. The re-isolation rate and enumeration of SE were lower in sodium butyrate and enrofloxacin treatments than positive control. In conclusion, sodium butyrate as an acidifier could be used as an environmentally friendly supplement when compared with enrofloxacin for treatment of SE infection in broiler chickens as it could reduce the disease picture severity, improve performance variables and decrease the intestinal colonization.
  Wafaa A. Abd El-Ghany , M.H. Awaad and S.R. Nagwa
  Two strains of Campylobacter jejuni (C. jejuni) representing biotype I and II were used for preparation of bacterin. Pure, sterile and safe watery aluminium hydroxide and incomplete Freund’s oil adjuvant bivalent bacterins were prepared. Both types of bacterins were evaluated in broiler chickens through subcutaneous (S/C) inoculation at one week old and boostered at three weeks of age. Results of immunoassay [mean Enzyme Linked Immuno-Sorbent Assay (ELISA) titres] and bioassay (clinical signs, mean lesion score, shedding and re-isolation rates as well as histopathological examination) proved that both types of bacterins were effective. However, oil type bacterin gave more protective effect than water type one.
 
 
 
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