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Articles by M.H. Rahman
Total Records ( 19 ) for M.H. Rahman
  R.S. Sultana , M.A. Bari , M.H. Rahman , M.M. Rahman , N.A. Siddique and N. Khatun
  Plantlets regeneration were achieved in callus culture of leaf segments from five days old field grown seedlings of Citrulus lanatus Thumb. Callus induction and plant regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in MS medium supplemented with 2.5 mg L-1 2, 4-D. After transfer of this callus on MS medium supplemented with 1.0 mg L-1 BA+0.2 mg L-1 NAA induce adventitious shoots and developed into plant by further subculture in the same medium. NAA (0.1 mg L-1) was found effective in the production of root. Plantlets were acclimatized and subsequently transferred to the field. Survival of the plantlets under ex vitro condition was 80%.
  M.Z. Rahman , M.G. Sharoar , Mohammad Nurul Matin , M.H. Rahman , M. Mizanur Rahman and M. Rezuanul Islam
  Banana cultivar ‘Mehersagar’ is a dessert banana clones cultivated all over the country. For the purpose of the production of disease free plantlets suitable for large-scale cultivation in the field, a micropropagation protocol based on multiple shoot regeneration has been established. Highly proliferative cluster of multiple shoots are obtained by culturing shoot tips from ex vitro grown healthy suckers on MS medium with different concentrations and combinations of cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (cw). The efficacy of these supplements on the rate of shoot multiplication was tested. Best results were obtained on MS+0.5 mg L-1 BAP+0.5 mg L-1 KIN+13% cw, where highest average number of shoots per explant was 8.2±0.236. When individual shootlets were separated and implanted in half strength MS medium with various concentrations of auxins, the better root formation were noted at 1.0 mg L-1 IBA with 6.10±0.359 roots per shoot. Then the rooted plantlets were transferred to polybags and about 90% of the acclimatized plantlets were survived when transferred to open field.
  M.Z. Rahman , M.H. Rahman , M.E. Haque , M.H. Kabir , S.L. Naher , K.M.K.B. Ferdaus , A.K.M. Nazmul Huda , M.S. Imran and M. Khalekuzzaman
  An experiment was conducted to find out a suitable banana (Ranginsagar and Sabari) based intercrop combination of potato and mustard in the research field of Institute of Biological Science of Rajshahi University, Rajshahi-6205, during Rabi season of 2003-2004. Average over the two years, it revealed that the highest yield of banana was observed in Ranginsagar + potato (64.72 t ha-1) and the lowest in sole mustard (1.92 t ha-1). Combinations of Ranginsagar + potato intercropping showed better performance than other combination and sole crop in respect of gross return (Tk. 735219 ha-1), net return (Tk. 359794 ha-1) and benefit cost ratio (1.95). The highest land equivalent ratio (LER) was 1.82 in Ranginsagar + Potato followed by Sabari + potato (1.79) and the lowest in Sabari + mustard (1.60). The economic return was more in intercropping than all sole crops.
  M. Q. Haque , M.H. Rahman , Fokhrul Islam , Jan Rijpma and M.M. Kadir
  A field experiment was conducted to study the effect of continued fertilizer, organic manure and mungbean residues on soil properties and yield of crops. The grain and straw yield of wheat, mungbean and T.aman were significantly influenced by different treatment combinations of organic and inorganic fertilizations. The highest average grain yield of Wheat var. Kanchan was 4.92 t ha-1 in the treatment T4 (N125, P25, K85, S25, Zn5, Mg20 and B2 kg ha-1). For mungbean (var. Binamoog-2) the highest grain yield of 1.06 t ha-1 was obtained with inoculum + (P10, K12 and S4 kg ha-1). The yield of T.aman (var. BRRI Dhan-32) was significantly increased when inorganic fertilizers were applied along with incorporation of mungbean stover. The highest average grain yield of rice 5.81 t ha-1 was recorded with N105, P16, K60 and S8 kg ha-1 + mungbean stover. The residual effect of cowdung and incorporation of mungbean straw as brown manure along with inorganic fertilizer was distinct. Economics of the fertilizer uses of the total products of two cropping cycles demonstrated that the highest net benefit of US $ 1998 ha-1 was obtained from treatment T3.3 (inorganic fertilizer for moderate yield + cowdung). There was no remarkable change in post harvest soil status during the period of study. However, there were considerable increase in available P but in case of K, the amount of K removed far exceeded that replenished through fertilization.
  M. Saiful Islam , M.A. Rahman , M.A. Salam , A.S.M.H. Masum and M.H. Rahman
  An experiment was carried out to study the growth and vegetable pod yield of edible podded pea as influenced by sowing time and spacing. The experiment consisted of two factors, which were three sowing times and six plant spacings. The results revealed that Nov. 23 sown plants showed higher leaf area index, leaf, stem and pod dry matter, plant height and vegetable pod yield than those of Nov. 8 and Dec. 8 sowing. Closest spacing produced the highest LAI, leaf, stem and pod dry matter, crop growth rate, plant height and vegetable pod yield. However, relative growth rate and net assimilation rate were the highest in widest spacing. Regardless of variation in sowing time and spacing, LAI, leaf and stem dry matter, CGR, RGR and NAR increased sharply up to 60 DAE and then declined but pod dry matter increased thereafter. Moreover, plant height increased rapidly up to 60 DAE and then slowly. The highest vegetable pod yield (10.26 t ha -1) was recorded from the Nov. 23 sowing with 30x20 cm2 spacing.
  M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman
  Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.
  N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah
  The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.
  N.A. Siddique , M.A. Bari , N. Khatun , M. Rahman , M.H. Rahman and S. Huda
  Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  N.A. Siddique , M.A. Bari , A.T.M. Naderuzzaman , N. Khatun and M.H. Rahman
  This paper deals with over one hundred plants of ethnobotanical importance, used by the village doctors especially village kaviraj of Barind Tract for medicinal purposes.This work aims at the preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines and also identification of endangered medicinal plants by questionnaire survey.The data presented in this paper has been collected by personal contact with the local people and herbal practitioners, obtaining the information with great difficulty because of their recticence in divulging the secret of the identity of plants of great traditional reputation.
  N.A. Siddique , M.A. Bari , Sharmin Shahnewaz , M.H. Rahman , M.R. Hasan , M.S.I. Khan and M.S. Islam
  Callus cultures were initiated from nodal segments on Murashige and Skoog[19] medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  R.S. Sultana , M.A. Bari Miah , M.M. Rahman , M.H. Rahman and M.U. Mollah
  Effects of different concentrations of sucrose, agar and different levels of pH on in vitro axillary shoot multiplication of Momordica charantea Linn. were studied in the present investigation. The nodal segments from the field grown plant were used as testing plant material. For maximum number of shoot induction and multiplication in MS medium  containing 2.0 mg L 1 BAP+0.2 mg L 1 NAA with 30 g L 1 of sucrose, 7 g L 1 of agar and 5.5-6.0 levels of pH proved more effective. When the medium having 30 g L 1 sucrose, the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for total number of shoot per culture and average length of shoots and that were 5.1±0.8 and 5.6±0.4 cm, respectively. The highest response of shoot proliferation from the nodal explant was observed on MS medium having 7 g L 1 of agar and the frequency was 100%. Among different levels of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5-6.0 levels. It was proved by the present investigation that in vitro growth and shoot multiplication was affected by sucrose, agar and pH on the shoot induction medium.
  M.S.U. Khan , M.M. Rahman , M.A. Samad , M. M. Khatun and M.H. Rahman
  The study was carried out to determine the efficacy of neem oil and common salt as curing agent to control biodegradation of green hides. A total number of nine intact hides were obtained from slaughtered cattle of 4 and 4 1/2 years of age. Three types of curing treatments were employed using various concentrations of neem oil and common salts. T1 provides 40% neem oil and 10% common salt, T2 includes 10% neem oil and 20% common salt and T3 contains 10% neem oil and 10% common salt. The hides of each treatment were kept at 10, 20 and 30 days storage periods. After every 10 days of storage hides were subjected to bacteriological examinations. Storage properties of hides were also determined by the evidence of spot, color changes and sliminess of the skin. The mean value of total bacterial count was recorded lowest 6.96±0.30 in T2. Total coliform counts were found almost nil in T2. None of the treatment was found effective to control the growth of staphylococci. No signs of bacterial spoilage in hides were evident in T1 upto 10 days of storage. However indication of some sliminess could be noticed in the regions of neck and butt after storage of 20 and 30 days. T2 exhibited more or less changes in color on the 10th day of storage and sliminess was noticed fairly distributed on the neck and belly regions on the 30th day of storage. T3 exhibited some noticeable changes in the butt region. Putrefactive action started and there was indication of tissue changes, which could be marked, from the 10th day of storage. At the butt region foul odor was perceptible and few hair slip was evidenced on the 30th day of storage. It is concluded that the treatment using combination of neem oil and common salt showed antibacterial activity against the growing contaminating putrefactive organisms but less effective against halophiles.
  M. M. Rahman , A.B.S.M. Osman Gani , M.A. Choudhury and M.H. Rahman
  The binding of dexamethasone phosphate (DP) and testosterone phenyl propionate, (TPP) two semisynthetic steroids, to bovine serum albumin (BSA) was studied by equilibrium dialysis method at 25°C and pH 7.4 with a view to have an insight into the competitive binding characteristics of these two drugs, when bound to BSA simultaneously. There was increase in free concentration of DP due to addition of TPP and vice versa during concurrent administration of these two drugs, thereby causing reduced binding of these two drugs to BSA. However, the free fraction was not increased up to a level as it was expected from direct competitive displacement. In absence of the site I specific probe (warfarin sodium), DP after being displaced by TPP or vice versa from its high affinity binding site (site II) rebound to its low affinity binding site (site I) on BSA. However, when the site I was sufficiently blocked by warfarin, the increment in the free concentration of the displaced drug was more prominent. This form of modified displacement has been referred to as site-to-site displacement.
  Sabina Yesmin , M.H. Rahman , M. Afzal Hussain , A.R. Khan , Farzana Pervin and M.A. Hossain
  For studying the load of the bacterium, Aeromonas hydrophila in swamps and artificial fish infection, four swamps were studied in Rajshahi University campus during November, 2001 to October, 2002. The load of A. hydrophila was found to vary from 1.16x107 to 4.90x107 CFU ml-1. In the artificial infection to the snakehead, Channa punctatus, the mortality rates were recorded as 100, 75, 25, 12.5 and 0% at 3.42x109, 3.42x108, 3.42x107, 3.42x106 and 3.42x105 CFU fish ml-1, respectively. The average bacterial load in the swamps (3.42x107 CFU ml-1) was slightly higher than that prevailed in the control pond (3.13x107 CFU ml-1). Swamps can be utilized at the present bacterial load for the fish culture.
  A.N.K. Mamun , M.N. Matin , M.A. Bari , N.A. Siddique , R.S. Sultana , M.H. Rahman and A.S.M. Musa
  The woody legume, Albizia lebbeck, commonly cultivated for social forestry in rural area of Bangladesh was selected for micropropagation by tissue culture technique. Cotyledon, nodal segment of in vitro grown seedlings and nodal segments of field grown mature tree were used as explants. With proper manipulation of cytokinin and auxin combinations and concentrations, it was possible to induce callus from three types of explants of Albizia lebbeck. Different combinations of BA with NAA and KIN with NAA were proved efficient media formulation for callus induction. Among all the hormonal supplements used BA-NAA combination with MS medium was proved best in all respect of callusing response in Albizia lebbeck. Among the explants the in vitro internodal segments were the best for callus induction. The highest 100% callus induction was observed in media having MS+2.0 mg L-1 BA+0.2 mg L-1 NAA and the fresh weight of callus was 1.3 g. Among these three explants of Albizia lebbeck only calli derived from cotyledon regenerated best shoots in MS media supplemented with BA singly or in combination with NAA and KIN. In respect of direct shoot regeneration nodal explants of Albizia lebbeck produced highest range of regenerated shoots.
  S.M. Mahbubul Alam , M.M. Rahman , M.H. Rahman and N.N. Rahman
  The interaction of tetracycline hydrochloride to Bovine Serum Albumin (BSA) at various temperatures and pH values using Equilibrium Dialysis (ED) method was studied. Scatchard analysis of the binding data revealed the presence of one high affinity binding site with k1 value of 1.67x106 M-1 and six low affinity binding sites with k2 value of 1.44x105 M-1 at pH 7.4 and 25°C. Site-specific probe displacement data suggested that warfarin site (site-I) is the high affinity binding site and benzodiazepine site (site-II) is the low affinity binding site on BSA for this drug. The high affinity binding site was found to be affected by temperature and pH of the medium. The thermodynamic data indicated that the binding process of tetracycline hydrochloride to BSA is spontaneous, exothermic and entropically driven. Electrostatic forces, hydrogen bonding, hydrophobic interactions and van der Waals forces are probably involved in the overall binding process of tetracycline hydrochloride to BSA. The affinity of this drug to BSA is dependent on the conformational changes of BSA caused by N-B transition.
  M.Z. Rahman , M.H. Rahman , M.U. Mullah , N. Nahar , R.S. Sultana , M.A. Bari and M. Hossain
  The effects of various cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (CW) alone or in combinations on shoot multiplication of banana cv. Anupom were investigated. The rate of multiplication varied in different treatments. Among the treatments, MS medium supplemented with 5.0 mg L-1 each of BAP + KIN and 13% coconut water produced the highest number of shoot per explant (5.8±0.154). The number of shoots responded for rooting and their survivability were found higher with IBA than NAA and IAA. MS medium supplemented with 1.0 mg L-1 IBA produced the highest number of roots per shoot (7.±0.245). Plantlets grown without any auxin in the medium gave the least number of roots/shoot (2.60±0.219). The rooted plantlets were successfully transferred in the polybags and finally were well established in the field.
  S.M. Bokhtiar , M.J. Alam , K. Mahmood and M.H. Rahman
  Field experiments were conducted to study the effects of integrated plant nutrient system (IPNS) on the yield and yield contributing parameters of sugarcane at Ishurdi, Thakurgaon and Sreepur under High Ganges River Flood plain, Old Himalayan Piedmont Plain and Madhupur Tract, respectively. The use of organic and inorganic fertilizer showed significant effect on the yield and yield attributes like tillers, millable stalk and juice quality of sugarcane. The results revealed that yield increase was to the extent of 22.86, 49.2 and 74.0 % at Ishurdi, Thakuragaon and Sreepur, respectively due to the application of 12.5 t ha -1 press mud/cowdung accompanying chemical fertilizers applied on the basis of soil test for HYG over present recommended rates of chemical fertilizer (as per FRGĀ“97) for MYG. Press mud alone increased 16-20 per cent cane yield over the dose of N, P, K, S, Zn and Mg fertilizers based on soil test for HYG at all locations. Incorporation of dhaincha (Sesbania aculeata) as green manure increased cane yield and contributed about 42-46 kg N ha -1 in soil.
  Sharmin Shahnewaz , M.A. Bari , N.A. Siddique and M.H. Rahman
  The present studies were carried out to evaluate the response of anthers of six rice genotypes to callus induction and plant regeneration. For callus induction Z2 media supplemented with 2, 4-D 0.5 mg l-1, NAA 2.5 mg l-1 and Kinetin 0.5 mg l- was used. Of the six genotypes, five gave good callusing response. Highest callus induction was observed in BRRI Dhan-29 (8.06%) and lowest in BR-10 (1.42%). Callus induction frequency varied from 1.42-8.06% depending on genotypes. Modified MS medium supplemented with Kinetin 0.5 mg l-1, BAP 2 mg l-1 and NAA 1.0 mg l-1 was used for plant regeneration. The results showed a significant genotypic difference in callus induction and plant regeneration. The regeneration of plantlet from anther derived calli ranged from 57-75% conclusion.
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