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Articles by M.G. AL-Saghir
Total Records ( 4 ) for M.G. AL-Saghir
  M.G. Al-Saghir
  Pistacia L. belongs to the family Anacardiaceae (cashew family), order Sapindales. Pistacia vera L. (cultivated pistachio) is by far the most economically important species in the genus. It has edible seeds and considerable commercial importance. The evolutionary history and the phylogenetic relationships between species within the genus are not well understood. A better understanding of these relationships is needed to make the species more useful for plant improvement or genetic studies. The objective of this perpestective is to provide additional insight into understanding the evolutionary history of Pistacia. In conclusion, Pistacia is a monophyletic genus and it contains two sections (Lentiscella and Pistacia) and it is originated in the Paleocene epoch. This is based on Anacardiaceae being pantropical in distribution with North and South America representing major diversification centers of the family including the geographical distribution of Pistacia. This perspective provides additional insight into understanding the evolutionary history of the genus Pistacia to make the species more useful for plant improvement or genetic studies.
  M.G. AL-Saghir , H.I. Malkawi and A. EL-Oqlah
  The objectives of this study were to locate and collect wild barley accessions of the species (Hordeum spontaneum C. Koch) from certain regions in northern Jordan (Ajloun area) to characterize the molecular diversity of the collected barley accessions by analyzing the DNA amplification products using AFLP and RAPD molecular marker methodologies; evaluate the AFLP and RAPD methods to be used in barley as genetic markers and improve such techniques as suitable strategies for barley germplasm characterization. Fifty samples representing five populations of Hordeum spontaneum C. Koch from northern Jordan (Ajloun area) were included in this study and were subjected to RAPD and AFLP molecular markers analysis. Twenty-seven RAPD primers produced 288 amplification products of which 90 (31.3%) were polymorphic and six AFLP primers produced 300 amplification products of which 155 (51.7%) were polymorphic. Genetic diversity was larger among than within populations.
  M.G. AL-Saghir and Abdel-Salam G. Abdel Salam
  The objectives of this study were to evaluate the genetic diversity of the North American soybean (Glycine max L.) accessions using Random Amplified Polymorphic DNA (RAPD) molecular marker; and to evaluate RAPD markers to be used in soybean as genetic markers and improve such techniques as suitable strategies for soybean germplasm characterization. Twenty soybean accessions were included in this study and were subjected to RAPD molecular markers analysis. Twenty-seven RAPD primers produced 210 amplification products of which 78 (27.3%) were polymorphic. In conclusion, this study reported a successful fingerprinting of G. max accessions using RAPD markers and demonstrated the usefulness of these markers in estimating the extent of genetic variation in Soybean germplasm.
  M.G. Al-Saghir
  This study was conducted to develop a rapid and efficient protocol for extracting high quality DNA from Pistachio trees suitable for PCR and molecular studies. Genomic DNA was extracted from 12 Pistachio trees using modified QIAGEN DNeasy Plant Mini Kit. The results showed that the modified protocol successfully produced a sufficient amount of DNA with high quality, which was highly confirmed by the purity index values of DNA samples (1.45 to 2.01). In conclusion, the modified protocol can produce high quality DNA from Pistachio trees suitable for PCR studies such as RAPD and AFLP and it can be easily adjusted for other Pistacia species.
 
 
 
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