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Articles by M.F.F. Abdullah
Total Records ( 3 ) for M.F.F. Abdullah
  N.A. Wahab , Z.M. Noor and M.F.F. Abdullah
  An E. coli yeast shuttle vector for the anchoring of heterologous protein to the yeast host’s cell wall was constructed. The vector PYDSM01 includes a DNA sequence constructed from the signal sequence from the yeast sucrose isomerase gene, a multiple cloning site and a DNA fragment encoding the carboxyl-terminal of the yeast cell wall protein 2 (CWP2). This construct was then inserted into the HindIII site on pGAD424, replacing the GAL4 fusion tag and the original MCS sequence. DNA sequencing confirmed the correct insertion of both signal and anchor proteins in the vector. To test for proper expression and functional anchoring to the cell wall, the coding sequence for a bacterial alpha-amylase enzyme was cloned into the vector and transformed into a yeast host. A total of 22 yeast transformant were recovered which were able to degrade starch, indicating successful expression and function of the bacterial alpha-amylase gene. Enzyme assay of the washed cell pellet and supernatant fractions indicate that the both activity and anchoring efficiency are variable.
  A. Abdul-Aziz , M.F.F. Abdullah and N.H. Hussain
  Acid Tolerance Response (ATR) in Shigella sonnei and Shigella flexneri was induced by pre-exposing the mid exponential phase cultures at sub lethal pH of 4.5 for 90 min before challenging them in lethal pH of 3.0. The ATR was exhibited by both species with S. flexneri displaying a higher tolerance to lethal acid killing. Analyses of protein profiles of both unadapted and adapted cells using 2-dimensional gel electrophoresis (2D-PAGE) revealed that the acid adapted cells of S. sonnei demonstrated differential expression of 19 Acid Stress Proteins (ASPs). Five of these proteins were synthesized de novo, 11 were upregulated while three other proteins were repressed. In S. flexneri, two proteins were synthesized de novo, four upregulated and two proteins were repressed. Two of these proteins with estimated molecular weights of 14.1 and 15.1 kDa and isoelectric points (pI) of 5.50 and 5.58, respectively were found to be consistently induced in both species. An inducible ATR in Shigella may protect the bacteria from environmental pH stress and aid in their pathogenesis. The detection of proteins important for ATR may present novel targets for antimicrobial intervention.
  A.L.M. Low , S.A.S. Mohamad and M.F.F. Abdullah
  Manure compost is a unique microenvironment which harbours potential new microorganisms that is able to produce a variety of anti-infective agents. However, the exploration of this ecosystem for actinomycetes and its bioactive secondary metabolites remained understudied. Therefore, this study aims to study the diversity of manure compost actinomycetes and to investigate their antimicrobial potential using conventional disc diffusion method and a modified resazurin microtiter based approach. A collection of 191 actinomycete isolates were recovered from 5 types of manure composts collected around Selangor, Malaysia. Utilizing a combination of micromorphological characteristics and 16S rRNA sequence analysis, the isolated actinomycetes were grouped into 12 genera within 9 families. Streptomyces spp. dominated the culture collection (79.1%) while the rest belonged to the non-Streptomyces group (20.9%), including an unusual isolate from the genus Verrucosispora. The evaluation of antimicrobial activities demonstrated that 21.5% of the isolates exhibited antagonistic effect against at least one of the test microorganisms with strong inhibition observed against fungal strains compared to pathogenic bacteria. A modified resazurin microtiter based assay was also developed and displayed higher sensitivity (40.0%) compared to the disc diffusion assay (26.0%). All ten actinomycete isolates which displayed narrow and broad spectrum effects also produce pigmented extracts. The results demonstrated that manure compost actinomycetes could be a promising source of novel bioactive agents and that the resazurin microtiter based assay is a more sensitive approach in screening antimicrobial properties of large numbers of microbial extracts.
 
 
 
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