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Articles by M.F. Hussein
Total Records ( 4 ) for M.F. Hussein
  S.I. Al-Mufarrej , M.F. Hussein , R.S. Aljumaah and A.R. Gar ElNabi
  During an outbreak of caprine abortion in a farm in Riyadh, Saudi Arabia, serum samples from 57 Aardi goats with recent history of abortion and stillbirths (herd 1) along with samples from 30 control goats without abortion history (herd 2) were analyzed for anti-Toxoplasma gondii antibodies using indirect Enzyme-Linked Immunosorbent Assay (ELISA) and Indirect Haemagglutination (IHA) tests. Antibodies against T. gondii were detected by ELISA in 63.2% of the goats in herd 1 and 50% of the control goats (herd 2). About >80% of the former goats had ELISA percent optical densities (O.D.%) ≥100 while >86% of the control goats were weakly positive with ELISA O.D.’s (%) <100. IHA tests were positive in 51.7% of herd 1 versus 30% of control goats. About 78% of all IHA positive goats in herd 1 had antibody titers ranging between 1:640-1:2560 including nearly 30% with titers ranging between 1:1280-1:2560. By contrast, all IHA positive goats in herd 2 had titers ranging only between 1:80-1:160, except one goat with a titer of 1:640 and none with higher titers. IHA tests for IgM antibodies using 2-mercaptoethanol treated sera revealed IgM antibodies in 16 (28%) goats with abortion history (herd 1) and none in control goats (herd 2). Focal pathological changes were observed in the fetal brain and the placenta of some of the aborted goats. These findings strongly suggest T. gondii as the cause of abortion in herd 1.
  M.F. Hussein , R.S. Al-jumaah , A.A. Alhaidary , M.A. Alshaikh , A. Gar ElNabi , O.B. Mohammed , A. Sawsan Omer and W.V. Macasero
  Total Platelet count (PLT), Mean Platelet Volume (MPV), Plateletcrit (PCT) and Platelet Distribution Width (PDW) were determined, along with hematological parameters, in blood samples from 8 captive mountain gazelles (Gazella gazella). The mean values of PLT, PCT, MPV and PDW were: 288.75±168.8 (x103 μL-1), 0.20±0.13%, 7.24±0.84 (fl) and 34.84±2.36%, respectively. The corresponding values for parallel red cell variables were 13.48±1.17 (1012 L-1) for Red Blood Cell count (RBC), 52.70±5.45 (fl) for Hematocrit (HCT), 39.00±1.07 for Mean Corpuscular Volume (MCV) and 19.86+0.32% for Red cell Distribution Width (RDW). Correlation analysis revealed highly significant correlations for PCT with PLT (p<0.0001), PCT with HCT (p<0.001) and PDW with MCV (p<0.001). This study is the first record of platelet counts and platelet indices in mountain gazelles.
  M.B. Magzoub , H.A. Al-Batshan , M.F. Hussein , S.I. Al-Mufarrej and M.Y. Al-Saiady
  This study was undertaken to determine the effect of dietary chromium supplementation on humoral antibody response and some blood constituents in hybrid tilapia fish (Oreohromis niloticus x O. aureus). Cr-yeast or Chromic Oxide (Cr2O3) were added to fish feed at the rates of 1 or 2 mg kg-1, while control fish received unsupplemented diet. Three hundred and forty fish (Avg. weight 37.8 g) were randomly assigned to 5 feeding regimens with two replicates each. Each replicate was reared separately. The fish were immunized with Sheep Red Blood Cells (SRBCs) at 0 day and re-immunized at 30 days of the experiment to determine primary and secondary humoral antibody responses, respectively. Blood samples were collected from 15 fish/replicate at 0 and 25 days to determine blood plasma constituents and at 5 days intervals during primary and secondary immunization up to 50 days to determine anti-SRBC antibody titers. The results shown decreased plasma glucose and increased plasma cholesterol concentrations at 25 days compared to 0 days in all groups. Increased triglyceride levels was noted in fish receiving Cr2O3 (1 mg kg-1) and decreased albumin: globulin ratio in those receiving Cr yeast (2 mg kg-1) at 25 days (p<0.05). No significant changes were observed in total protein, albumin and total globulin concentrations due to either Cr treatment or feeding duration. Anti-SRBC antibody titers were significantly higher in Cr-supplemented groups versus control, with highest titers in fish receiving 2 mg kg-1 Cr-yeast supplement during secondary immunization. These findings indicate that the inclusion of Cr in the feed significantly augments humoral antibody response of tilapia.
  M.B. Magzoub , H.A. Al-Batshan , M.F. Hussein , S.I. Al-Mufarrej and M.Y. Al-Saiady
  This study was undertaken to determine the effect of dietary Chromium (Cr) supplementation on performance, body chemical composition, plasma glucose, Hepatosomatic Index (HSI) and liver glycogen in hybrid tilapia (Oreochromis niloticus x O. aureus). Cr-yeast or Chromic Oxide (Cr2O3) were added to fish feeds at the rates of 1 or 2 mg kg-1 while control fish received an unsupplemented diet. About 1400 fish (avg. weight 18.6 g) were randomly assigned to 5 feeding regimens, each consisting of four replicates. Each replicate was reared separately. Body Weight (BW), Feed Intake (FI), Growth Rate (GR) and Feed Efficiency Ratio (FER) were measured at days 14, 28, 42, 56, 70 and 84. Chemical composition, HSI and liver glycogen were determined at days 0, 30 and 60. Plasma glucose was measured after starving the fish for 24 h and at 1, 2, 3, 4, 6, 8 h after feeding. The results showed increased BW, FI, GR, FER, total protein, ether extract, gross energy, plasma glucose and liver glycogen and decreased ash percent in all groups. Comparison of the overall means of these variables in different feeding groups showed that BW, FI, GR and FER were significantly lower in fish receiving 2 mg kg-1 Cr-yeast while overall mean values of ether extraction and gross energy were significantly higher in fish receiving 1 mg kg-1 Cr2O3. On the other hand the highest overall mean value for liver glycogen was recorded in fish receiving 2 mg kg-1 Cr. No significant changes were observed in moisture, ash, total protein, plasma glucose or HSI. These findings indicate that the inclusion of Cr in fish feeds can lead to improvement of tilapia chemical composition and physiology, namely ether extract, total energy and liver glycogen.
 
 
 
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