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Articles by M.F. Ghaly
Total Records ( 3 ) for M.F. Ghaly
  S.A. El-Sherbiny , Y.M. El-Ayoty , M.F. Ghaly and N.S. Fleafil
  Optimization of S. neyagawaensis N60 (Egyptian isolate) for the production of natural antialgal substance was carried out. The Clear Inhibition Zone (CIZ) in the different algal species indicated the maximum biological activity of metabolite was attained at 6 g L-1 maltose 1.05 g L-1 NH4Cl and 1 g L-1 K2HPO4 under pH 6.5, temperature 28°C and incubation period 7 days. For microanalysis, xylene was the most efficient solvent for extraction of the lytic substance which has one spot under UV lamps at RF 0.65 using TLC. Identification of the antialgal substance produced by S. neyagawaensis was carried out on the basis of elementary analysis, IR, mass and NMR spectra. The earlier analysis emphasized that the molecular weight equal 369.45 kDa with chemical formula C19H21NO6 (Anthracidin A). Different concentrations of Anthracidin A were tested against Anacystis nidulans revealed that chlorophyll a, nucleic acids were reduced with increasing the concentration of Anthracidin to 40 μg mL-1.
  Kh.A. El-Dougdoug , M.F. Ghaly and M.A. Taha
  An antiviral producing Streptomyces species were isolated from soil rhizosphere in Zagazig province of Egypt. In order to identify the Streptomyces strains, morphological, physiological, biochemical and antagonism tests were performed. The Egyptian isolates of Streptomyces were found to be a species of calvus, canarius, vinaceusdrappus, nogalater and viridosporus. The Streptomyces spp. were grown in glycerol asparagine broth medium and the culture supernatants obtained were 0.45-μm filter. These isolates were tested in two experiments for their ability to control a Cucumber Mosaic Virus (CMV). In the 1st experiment, one half of leaves of Chenopodium amaranticolor were treated with Culture Filtrate (CF) followed by CMV inoculation on both halves. In the 2nd experiment, the first pair of Cucumis sativus leaves were treated CF with CMV mechanically inoculated onto one leaf, the other non-treated leaf was CMV inoculated after 7 days of treatment. In 1st experiment, CF treatment was able to considerably reduced the number of local lesion and in 2nd experiment, plants treated with CF showed variable visible viral symptoms compared with the broth media treated control 15 days post inoculation and remained symptom less throughout the study period. Such five Streptomyces species identified were able to produce an antiviral component in the culture filtrate, non phytotoxic and effective in local as well as systematically control of CMV infection.
  S.A. El-Shirbiny , M.F. Ghaly , Y.M. El-Ayoty and N.S. Fleafil
  The antialgal activity of 107 Actinomycete isolates were isolated from different localities in El-Sharkia, El-Dakahliya and El-Meniya governorates. Egypt. Isolate No. 40 (isolated from Ekhtab. Aga, Dakahliya) was selected and identified as Streptomyces endus N40. The optimum culture conditions for the production of the antialgal metabolite were studied. It has been found that the optimum incubation temperature for the production of the antialgal metabolite from Streptomyces endus N40 was 28°C after incubation period (8 days) at pH 6.5. The most favorable carbon, nitrogen, phosphorus, microelement and vitamin sources were maltose (6 g L-1), asparagine (1.486 g L-1), K2HPO4 (I g L-1), FeSO4.7H2O (0.01 g L-1) and (inositol) (0.01 mg L-1), respectively. The maximum antialgal activity was obtained against Anabaena sp. Anabaena flos-aqueae, Nostoc sp. and Anacystis nidulans. The antialgal substance had extracted using xylene solvent Formulation and identification of the antialgal substance that produced by Streptomyces endus N40 was carrying out using IR, Mass, NMR spectra and elementary analysis and these results were emphasized that the molecular weight equal 279.33 KDa with chemical formula (C15H21NO4) and identified as Niromycin A.
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