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Articles by M.E. Kooffreh
Total Records ( 3 ) for M.E. Kooffreh
  E.V. Ikpeme , O.U. Udensi , U.B. Ekaluo , M.E. Kooffreh , C.M. Okolo , P.B. Ekpo and N.C. Ogbonna
  The utilization of molecular markers-assisted genetic diversity study is critical and imperative for a more robust selection and breeding programmes in aquaculture. Twenty C. gariepinus species were randomly selected, 5 each from four Rivers/locations-Okurikan (OKU), Lemna (LMN), UNICAL Fish Farm (UFF) and MCC cultured fish site, in Cross River State, Nigeria for genetic diversity study using RAPD technique. Genomic DNA extraction and purification from caudal fin tissue sample was performed using ZR Genomic DNA tissue extraction Kit, primed using OPA09, OPA11 and OPA13 RAPD primers and amplified using PCR. The amplified fragments were ran on gel electrophoresis and visualized under ethidium bromide dye. Thirty four bands were generated by the three RAPD primers from the four C. gariepinus populations of which 28 were polymorphic, accounting for 82.35%. Polymorphism revealed by each fish population ranged from 92.86-100% while, gene diversity estimates ranged from 0.246±0.16-0.385±0.09. Intra-population similarity index ranged from 42.49-78.4% while, the inter-population similarity index across all population combinations ranged from 52.32-71.69%. These result also revealed that as genetic distances increases, genetic identities narrows between the wild and the cultured species. UPGMA dendrogram generated two major clusters with C. gariepinus species from OKU, UFF and MCC in cluster-1 and C. gariepinus species from LMN in cluster-2. Implicitly though, our results suggest that there were several genetic diversity in the populations studied, especially between the cultured and the wild, which could encourage selection of C. gariepinus from OKU and LMN populations for the purpose of breeding and improvement.
  M.E. Kooffreh , E.V. Ikpeme , E.E. Ekerette and N.O. Eyo
  Morphological and behavioral genetic traits of simple inheritance indicate ethnic variation and have been widely employed in population variation studies. Five selected genetic traits of simple inheritance, ABO and Rh (D) blood phenotype was investigated to establish the inheritance pattern, prevalence and to assess association between the traits, ABO rhesus phenotypes and gender of 45 unrelated families living in Calabar. Blood group O was the most prevalent (55.2%) followed by B (21.6%), A (18.8%) while the least was AB (4.4%). The majorities (91.6%) were Rh (D) positive and 8.4% were Rh (D) negative. The frequency of the 5 genetic traits were earlobe (69.2% free earlobe, 30.8% attached earlobe), hand-clasping (51.6% right hand-claspers, 48.4% left hand-claspers), dimples (21.2% had facial dimples, 78.8% had no dimples), mid-digital hair (presence in 92%, absence in 8%) and tongue rolling (48.4% rollers, 51.6% non-roller). There was association between earlobe and blood group, sex and hand clasping, sex and facial dimples and between tongue rolling and sex. Chi-square analysis of the inheritance pattern of these genetic traits provides strong evidence for a familial and probably a genetic component in the control of these traits. The pattern also showed that the traits are inherited in dominance versus recessive manner.
  E.V. Ikpeme , U.B. Ekaluo , M.E. Kooffreh and O. Udensi
  This study was aimed at qualitative evaluation of the ethanol seed, leaf and pulp extracts of C. papaya for bioactive compounds and also to investigate their effect on the haematology in male albino rats. A 3x4 factorial experimental layout using randomized complete design was adopted. Results show that the phytochemicals found in seed, leaf and pulp were almost the same but however, in varying proportions. Present result also revealed that there were significant effects (p<0.05) of the extracts on the heamatology of the treated rats, which was blamed on the varying and different variants of bioactive compounds found in the extracts they were administered with. Suggestively, C. papaya extracts could be used to enhance the production of selected blood parameters, taking issue of dosage into consideration.
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