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Articles by M.C.L. Almeida
Total Records ( 3 ) for M.C.L. Almeida
  G.F. Dire , J.C.S. Oliveira , S.D.D. Vasconcelos , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , M.L. Fernandes and M. Bernardo-Filho
  The aim of this study was to assess the effect of an extract of Sechium edule on the labeling of blood elements with 99mTc. A freshly extract of Sechium edule was administered to Wistar rats during 7 days. After that, samples (0.5 mL) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C in a well counter. Due to the analysis of the results it was observed a decrease in the uptake of %ATI by the blood cells in the diabetic group treated with chayotte (89.96±5.16) in comparison to the diabetic group (97.16±1.26). Related to the %ATI binding in the IF-P it was noticed a decrease in the efficiency of radiolabeling in the diabetic group (from 80.22±5.50 to 71.16±1.97) and in the diabetic group treated with the extract (from 80.22±5.50 to 70.87±4.10). Due to the %ATI binding to the IF-C it was observed a decrease in the group treated with chayotte extract (from 91.08±4.10 to 67.26±2.20), in the diabetic group (from 91.08±4.10 to 71.26±2.46) and in the diabetic group treated with chayotte (from 91.08±4.10 to 71.51±3.38). In the light of the results the referred extract has an oxidant action. We suggest that the referred extract may induce the generation of activity metabolites with direct action on the labeling process probably acting in the cell membrane and in the binding sites protein together with an oxidative stress present in diabetes.
  G.F. Dire , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , J.C.S. Oliveira , S.D.D. Vasconcelos , M.L. Fernandes and M. Bernardo-Filho
  We evaluated the influence of a chayotte(Sechium edule) extract (macerated) and diabetes status on the bioavailability of 99mTcO4Na. In this study, in the biodistribution analysis, the 99mTcO4Na (0.3 mL) was administrated into female Wistar rats (diabetes and no diabetes induced) which had drunk or not the extract (macerated) for 7 days. After 10 min, animals were sacrificed, the organs were isolated, the radioactivity determined in a well counter and the percentages of radioactivity (%ATI) in the organs was calculated. The analysis of the results has indicated an increase in the %ATI in the group treated with chayotte extract (0.0040±0.0010 to 0.0060±0.0010). In the diabetes group it was observed a decreased in the %ATI (0.0040±0.0010 to 0.0030±0.0010) as well as in the diabetes group treated with chayotte extract (0.0040±0.0010 to 0.0020±0.0003). Due to the %ATI in the muscle it was verified that there was an increase of the %ATI in the group treated with chayotte extract (0.0090±0.0040 to 0.0230±0.0070) and in the diabetes group treated with chayotte (0.0090±0.0040 to 0.0180±0.0230). It was observed a difference between the group treated with chayotte to the diabetes group (0.0230±0.0070 to 0.009±0.0040) and between the diabetes group to the diabetes group treated with chayotte extract (0.0090±0.0040 to 0.0180±0.0230). Related to %ATI in the spleen by the light of the results it was detect a difference in the uptake of sodium pertechnetate between the group treated with chayotte extract to the diabetes group treated with the referred extract (0.0290±0.0013 to 0.0480±0.0160). Due to the biodistribution it was related that an extract of eggplant was capable of altering the bioavailability of 99mTcO4Na different of a cauliflower extract which has not been able to alter it. It is possible to suggest that some components of chayotte extracts present an oxidant power able to alter the biodistribution of 99mTcO4Na, as a tip, we speculate that the referred extract when metabolized in the liver may produce reactive metabolites with oxidant properties linked to the stress which is generated by diabetic status, this fact could justify by the increase of %ATI in the brain, muscle and spleen which probably may be due to the producing of AGEs in diabetes status.
  G.F. Dire , S.D.D. Vasconcelos , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , J.C.S. Oliveira , M.L.Fernandes and M. Bernardo-Filho
  The use of natural products occurs around the world. The labeling of blood constituents with technetium-99m (99m Tc) has been influenced by natural extracts and oxidative stress. Some studies suggested that maternal diabetes can affect the embryology environmental and this fact could help to elucidate that the oxidative stress may be related to the disturb of the gene expression which is essential in the control of the ontogenetic processes. We evaluated the influence of a chayotte (Sechium edule) extract on the labeling of blood elements with 99m Tc in diabetic female rats. The animals were treated with chayotte during 7 days and samples of blood were withdrawn. The samples were incubated with stannous chloride and with 99mTc. Plasma(P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble (SF) and insoluble fractions (IF) separated. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C in a well counter. In the diabetic group it was observed an increase in the radioactivity in BC (from 43.65 ± 1.83 to 59.47 ± 1.83 and in the IF-BC (from 26.22 ± 23.58 to 73.28 ± 23.58). It was noticed that the referred extract has normalizing the efficiency of radiolabeling in the diabetic animals which have received the referred extract. The effect of chayotte extract probably, could be explained by the metabolization of the chayotte that would be capable of inducing the generation of active metabolites with oxidant properties probably altering the activity of cell membrane.
 
 
 
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