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Articles by M.B. Achenef
Total Records ( 2 ) for M.B. Achenef
  M.B. Achenef , A.K. Arifah , Y.M. Goh , A.Q. Sazili , O. Fauziah , Z.A. Zakaria , A. Zuraini and M.N. Somchit
  Conjugated Linoleic Acids (CLAs) are group of positional and geometric isomers of octadecadienoic acid with conjugated double bonds and believed to have many health promoting effects. The present study focused on the quantitative analysis of CLAs in liver, Superficial Pectoral (SP), Longissimus Dorsi (LD) and Semimembranosus (SM) muscles of local Malaysian Kedah-Kelantan (KK) cattle slaughtered for human consumption. Fatty acids were extracted using Folch method and determined by gas chromatography. The average content of CLAs in the liver, SP, LD and SM muscles were 38.71, 18.24, 11.03 and 13.04 mg/100 g of sample, respectively. The quantity of CLAs in the liver was significantly (p<0.05) higher than other samples. The percentages of cis-9, trans-11 CLA isomer were 63.39, 76.04, 90.66 and 82.82% of total CLAs in the liver, SP, LD and SM muscles, respectively. Positive correlations between CLAs and trans-11-octadecenoic acid concentration were observed in all samples. This study confirmed that meat from KK cattle could be the potential source of CLA but still its content has to be improved to make their meat more beneficial for consumers.
  M.B. Achenef , A.K. Arifah , Y.M. Goh , A.Q. Sazili , O. Fauziah , A. Zuraini , M.N. Somchit and Z.A. Zakaria
  This study was focused on the quantitative analysis of Conjugated Linoleic Acids (CLAs) in rumen liquor and assessment of the relationship between CLAs and other carbon 18 fatty acids and rumen pH of cattle slaughtered for meat consumption. The fatty acids were extracted using modified Folch method and determined by gas chromatography. The mean (n = 22) level of CLAs in the rumen liquor was 14.97 ±7.46 mg/100 mL. Cis-9, trans-11(c9, t11) was the dominant isomer of CLA followed by trans-10,cis-12 (t10, c12). The level of CLAs was positively correlated with the concentration of octadecatrienoic (18:3), cis-9, cis-12 octadecadienoic (18:2), trans-11octadecenoic (18:1) and octadecenoic (18:0) acids. Ruminal pH was positively correlated with the amount of c9, t11 and total CLA and negatively correlated with t10, c12 CLA isomer. All these correlations and relationships indicated that CLAs production in the rumen is dependent on availability of carbon 18 fatty acids and pH of the rumen liquor.
 
 
 
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