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Articles by M.A. Rasadah
Total Records ( 2 ) for M.A. Rasadah
  M. Kamal NH. , A. Zulkhairi , A.H. Hafizah , F. Fazali , A.K. Khairul Kamilah , M.A. Rasadah , M.S. Zamree and M.A.M. Shahidan
  Endothelial cell injury due to inflammation and oxidative stress are the hallmark of early pathologic events of atherosclerosis. Antioxidants derived from natural sources have been extensively used to prevent oxidative stress. The purpose of this study was to investigate the cytoprotective effect of Anacardium occidentale Aqueous Extract (AOE) against H2O2-induced HUVEC injury and its anti-inflammatory potential induced by TNF-α in vitro. HUVEC was exposed with various concentrations of H2O2 (0-700 μM) and it was observed that 250 μM of H2O2 reduced cell viability by 50% (IC50) as denoted by MTT assay. Using the above concentration as the PC, the cells were pretreated with AOE at various concentration (50-700 μg mL-1) for 30 min followed by 24 h incubation with H2O2 (250 μM) or TNF-α (10 ng mL-1), respectively. AOE was found to be not toxic to the cells as no inhibitory concentration (IC50) obtained. AOE (100-300 μg mL-1) protects cellular damage and prevent microsomal lipid peroxidation in H2O2-induced HUVEC indicated with low MDA levels. The AOE at similar concentrations also suppressed the production of VCAM-1, ICAM-1, MCP-1 and M-CSF in TNF-α-induced inflammation whereas NF-κB p65 translocation into nucleus was observed inactivated. These data suggested that AOE possessed antioxidative properties and attenuate the initial stage of atherogenesis in vitro. Inhibition of NF-κB activation could be the possible underlying mechanism in modulating early events of atherogenesis.
  S.K. Ihsan , H.A. Zulkhairi , M.A. Abdah , J.A. Daryl , I. Noramalina , M.A. Rasadah , M. Shahidan , M.S. Zamree , K.A.K. Kamilah , N.H. Mohd Kamal , A. Azrina , M.E. Norhaizan , M.A.M. Aris and M.B. Taufik
  The aimed of this study was to investigate the antioxidant properties of T. crispa as well as its ability to attenuate the release of oxidant markers in induced oxidation in Human Umbilical Vein Cells (HUVECs). In vitro studies of antioxidant properties of T. crispa showed the DPPH scavenging activity of T. crispa Aqueous (TCAE) and Methanol Extract (TCME) were 82±1.78 and 73±1.01%, respectively. FRAP value of TCAE and TCME were 1.04±0.27 and 1.64±0.06 mmol L-1, respectively. Total Flavonoids Content (TFC) of TCAE and TCME were 205.58±3.5 and 223±10.49 mg QE g-1 sample, respectively while Total Phenolics Content (TPC) of TCAE and TCME were 32.58±0.68 and 41.64±0.97 mg GAE mg-1 sample, respectively. The antioxidant enzymes activities (CAT, SOD and GPx) in HUVECs treated with T. crispa extracts to counter the oxidative effect by hydrogen peroxide (H2O2) and lipid peroxidation activity by Malondialdehyde (MDA) were also measured. The result showed TCAE and TCME at a concentration ranges from 100-600 μg mL-1 significantly increased (p<0.05, 0.01) the level of CAT, SOD and GPx. Results of MDA assay showed significant reduction (p<0.05, 0.01) of MDA level in HUVECs treated with TCAE and TCME. Taken together, the findings suggest that T. crispa extracts can effectively protect HUVECs against oxidative stress by H2O2 which might be of importance in the treatment of cardiovascular diseases.
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