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Articles by M.A. El-Harairy
Total Records ( 2 ) for M.A. El-Harairy
  I.T. El-Ratel , A.E. Abdel-Khalek , M.A. El-Harairy , Sara F. Fouda and Lamiaa Y. El-Bnawy
  Background and Objective: Green tea has very strong antioxidant, antibacterial, antiviral, enzyme inhibitory, antiradiation and anticancerous properties. This study aimed to evaluate the efficacy of different levels of green tea extract on reproductive performance, hematology, lipid metabolism and histological structure of liver and kidney of rabbit does. Methodology: Total of 30 mature does and 10 fertile bucks of New Zealand White (NZW) rabbits were divided into 3 groups, 10 in each. The 1st group was control, while second and third groups were given, 1 mL distilled water containing 200 and 400 mg kg–1 b.wt. of green tea extract, respectively, orally for 30 days prior to natural mating. Doe weight at start and end of treatment, mating, kindling and weaning and kid weight at birth and weaning were recorded. Doe litter size and kid viability rate at birth and weaning were calculated. Hematological parameters and lipid profile were determined in blood plasma of does after weaning. Histological structure of liver and kidney of does was examined. Results: Doe body weight was higher in control and second than in third group at end of treatment, mating and kindling (p<0.05) and at weaning (p>0.05). Kindling rate, gestation period and litter size of does and body weight and viability rate of kids did not differ. Hemoglobin concentration, hematocrit, mean corpuscular volume and white blood cells count increased (p<0.05), while platelet count decreased (p<0.05) in third than in control and second groups. Plasma total lipids, total cholesterol, triglycerides and high density lipoprotein concentrations decreased (p<0.05), while low density lipoprotein concentration increased (p<0.05) in second and third than in control group. No pathological legions were noted in the histological structure of liver and kidney. Conclusion: Daily oral administration of rabbit does 30 days prior to mating with green tea extract at levels of 200 or 400 mg kg–1 is recommended to improve reproductive performance and lipid metabolism of rabbit does without adversely effects on hematological parameters and liver and kidney function.
  M.A. El-Harairy , I.M. Abd El-Razek , E.A. Abdel-Khalek , S.M. Shamiah , H.K. Zaghloul and W.A. Khalil
  This study was conducted to determine the effect of different type and levels of antioxidant supplementation (0.4 and 0.8 mM from glutathione, GSH or 0.5 and 1.0 g L–1 extender from ascorbic acid, AA) as compared to control, on characteristics of camel epididymal spermatozoa stored at 25°C (room temperature) for 0, 2, 4 and 12 h or at 5°C (cool temperature) for 0, 12, 24 and 48 h. Testis of camel were collected after animal slaughtering and placed immediately into plastic bag into ice box at 5°C. Epididymal spermatozoa were collected by aspiration from tail and extended with tris-egg yolk extender. Results of epididymal spermatozoa stored at 25°C showed improvement in livability (p<0.05) and abnormality (p≥0.05) with GSH (0.4 mM), while sperm motility and curling spermatozoa improved (p≥0.05) with AA (0.5 g L–1). Storage at 5°C improved (p<0.05) motility, livability and curling spermatozoa with GSH (0.4 mM), while sperm abnormality improved (p<0.05) with AA (1 g L–1). At different incubation times at 25 or 5°C, percentages of motility, livability and curling spermatozoa decreased (p<0.05) and of sperm abnormality increased (p<0.05) by increasing storage time. The effect of interaction between antioxidant supplementation and storage time on all sperm characteristics studied was not significant. In conclusion, supplementation of tris-egg yolk extender with GSH (0.4 mM) or AA (0.5 g L–1) has a vital role in maintaining function, morphology and membrane integrity of epididymal camel spermatozoa stored at 25°C for 12 h or at 5°C for 48 h, respectively.
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