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Articles by M.A. Zainal Abidin
Total Records ( 3 ) for M.A. Zainal Abidin
  Ali A.A. Mustafa , Radziah Othman , M.A. Zainal Abidin and V. Ganesan
  A glasshouse study was conducted to investigate the growth response of sweet corn (Zea mays) to mycorrhizal fungi inoculation over different plant ages (2, 4, 6, 8 and 10 weeks) and to determine the interaction between the host and mycorrhizal fungus on mycorrhizal development, using soil substrate as inoculum for Glomus mosseae. Inoculation had no significant effect on growth parameters in plants harvested at early ages in terms of plant height, total root length, root and shoot dry weights. The significant effect of mycorrhizal inoculation was observed on plants of eight weeks old. Percentage of mycorrhizal colonization and number of spores increased significantly at plants of 2 weeks old (24.1 and 39.2%), respectively while; the highest AMF spores level was recorded at plants of four weeks old. Inoculation with G. mosseae enhanced growth of sweet corn by increasing concentration of N, P and K (24.2, 8.4 and 18.2%), respectively. We concluded that the mycorrhizal inoculation need a time (not less than one month in sweet corn) until showed the beneficial effects on host plant to be desired.
  M.M. Begum , M. Sariah , A.B. Puteh and M.A. Zainal Abidin
  A study on seed-borne infections of soybean [Glycine max (L.) Merrill] demonstrated the infectivity of 17 fungal species belonging to 11 genera using blotter and potato dextrose agar methods. Among them Colletotrichum truncatum, Diaporthe phaseolorum var. sojae and Fusarium oxysporum f. sp. glycines were found in higher frequencies and well established within and on the external surfaces of seeds. Histopathology of C. truncatum infection in seeds of soybean was examined under Light Microscopy (LM) and Scanning Electron Microscopy (SEM). No mycelium and acervulus were observed in any tissues of asymptomatic (healthy) seeds. Seeds colonized with C. truncatum produced irregular gray spots with black specks. C. truncatum produced compact dark mycelium both intra- and intercellularly in the seed coat, cotyledon and embryo. Mycelial growth was more abundant in the hourglass layer of the seed coat and hypodermis, where large inter-cellular spaces were present. Acervuli with setae and abundant hyaline sickle-shaped conidial masses were observed abundantly on the surface of infected seeds. Similar observations were found beneath the inner layers of the seed coat and upper surfaces of embryo and cotyledonary tissues. Brown conidial masses were produced during incubation and liberated in the form of ooze resulting in maceration and disintegration of the parenchyma tissues of the seed coat, cotyledon and embryo.
  M.M. Begum , M. Sariah , M.A. Zainal Abidin , A.B. Puteh and M.A. Rahman
  Histopathological studies on natural infections by Fusarium oxysporum f. sp. glycines in soybean seeds were conducted using Light Microscopy (LM) and Scanning Electron Microscopy (SEM). Asymptomatic (healthy) seeds were found free from pathogens. Infected seeds were slightly irregular in shape and appeared whitish moldy. The fungus mycelia colonized the external surface and inner tissues of the seed coat, but not in cotyledon or in embryo. Upper surfaces of seeds showed profuse colonization by mycelia and seed coat tissues became ruptured and distorted in the severely infected seeds. Vigorous mycelial growth was found in the hourglass layer of the seed coat. Effect of artificial inoculation on soybean seed germination and seedling survivability under glasshouse conditions was also studies. F. oxysporum f. sp. glycines reduced seed germination and seedling survivability by 40% and caused pre-emergence damping off of seedlings. Trichoderma harzianum isolate UPM40 and Pseudomonas aeruginosa isolate UPM13B8 were most effective candidates in inhibiting the mycelial growth of F. oxysporum f. sp. glycines in vitro.
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