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Articles by M.A. Qadeer
Total Records ( 6 ) for M.A. Qadeer
  Ikram-ul-Haq , Uzma Hameed , Kiran Shahzadi , M. Mohsin Javed , Sikander Ali and M.A. Qadeer
  The present study deals with the optimization of cultural conditions for Cotton Saccharifying Activity (CSA) of cellulases by Trichoderma harzianum UM-11 using shake flask technique in 250 mL Erlenmeyer flasks. Among the different fermentation media tested, Carboxymethyl Cellulose (CMC) with Mineral Salts Solution (MSS) gave the best CSA. Cotton saccharification was optimal when 25 mL of the medium was incubated at 28°C for 96 h. The maximum CSA (0.528 U/mL/h) was found at initial pH 6.0. The level of inoculum was optimized at 5.0x107 conidia/25 mL medium.
  Ikram-ul-Haq , Nuzhat Inam , Sikander Ali and M.A. Qadeer
  Microbial examination of 50 samples of both packed and unpacked butter was carried out. The samples were examined for total viable count, mould and yeast count, spore formers and coliform. The microbial load in the unpacked sample of butter was highest i.e., 3.8 × 106/gm - 6.6 × 106/gm. The coliform count was found maximum in unpacked sample and one of the packed sample of butter (Kausar brand) i.e., 39/ml. The unpacked sample of butter contained highest number of aerobic spore formers i.e., 280/gm. The anaerobic spore-formers were found absent in 20 samples of butter and the rest contained in the range of 0-170/gm. The yeast cultures were found only in the sample of Lyallpur and Kausar butter. The mould count of these samples ranged from 0-280/gm.
  Sikander Ali , Ikram-ul-Haq , M.A. Qadeer and Javed Iqbal
  Sixteen different cultures of Aspergillus niger were isolated from different soil samples. These isolates of Aspergillus niger were evaluated for citric acid fermentation in shake flask. Sucrose salt media was used and the volume of fermentation medium was kept at 25 ml. The cultural conditions such as pH (3.5), temperature (30°C), incubation period (8 days) and sugar concentration (15%), were optimised.
  Aamir Ishaq , Sikander Ali , Ikram-ul-Haq and M.A. Qadeer
  The present study is concerned with the time course profile of citric acid fermentation by Aspergillus niger and its kinetic reletions. Maximum amount of anhydrous citric acid (70.60 g l-1) obtained, 144 h after inoculation, with a sugar consumption of 88.40 g l-1. The dry weight of mycelia was 17.39 g l-1. On the basis of comparison of kinetic parameters namely the product and growth yield coefficients (Yp/s, Yp/x), volumetric rates (Qq) and specific rate constants (qp), it was observed that mutant strain of Aspergillus niger GCB47 was a faster growing organism and has the ability to hyper produce citric acid.
  Ikram-ul- Haq , Kiran Shahzadi , Uzma Hameed , Muhammad Mohsin Javed and M.A. Qadeer
  The present study deals with isolation, screening and optimization of cultural conditions for biosynthesis of cellulases by Trichoderma harzianum using agricultural byproduct. Twenty different strains of Trichoderma harzianum were isolated from different soil samples by serial dilution method. Of all the strains tested, KM07 gave maximum production of cellulases by solid-state fermentation. Different agricultural byproducts such as wheat bran, wheat straw, rice bran, rice husk and soybean meal were tested for the production of cellulases and wheat bran was found to be the best substrate. The production of enzyme was significantly improved as the wheat bran was moistened with mineral salts CMC solution (ratio 1:1). The cultural conditions such as temperature 28°C and pH 6.5 were also optimized. The production of cellulases was maximum 72 h after the inoculation.
  Ikram-ul-Haq , H. Ashraf , S. Omar and M.A. Qadeer
  The present study is concerned with the production of amyloglucosidase by Aspergillus niger GCUCM-36. Effect of addition of different carbon sources and nitrogen sources on the production of enzyme was investigated. The enzyme formation was maximum (1180 IU/g/min) in the presence of glucose (1.0%) and NH4Cl (1.5% nitrogen). The production of enzyme reached maximum, (1180 IU/g/min) at 48 hours after incubation.
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