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Articles by M.A. Islam
Total Records ( 27 ) for M.A. Islam
  M.J. Islam , M.A. Islam , S. Akter Tania , S.R. Saha , M.S. Alam and M.K. Hasan
  Twenty two garlic genotypes of local and exotic origin was trailed during the period from November 27, 2000 to March 31, 2001 at SRC Bogra. The results indicated that the germplasm differed significantly as to the different morphological attributes, yield and other desirable traits. The height of plants varied from 42. 50-67. 33 cm whereas, there was no difference as to the number of leaves/plant. Length and width of individual bulb differed from 2.40-3.20 and 2.10-2.71 cm, respectively. The number of cloves/bulb and individual bulb weight were also varied significantly. The entries showed variable degree of pungency. Per hectare yield (t) of garlic genotypes varied from 2.18-6.29, being lowest in GC003 and highest in GC007. Considering pungency as a positive trait of selection and yield potentiality also with other parameters, the lines GC002, GC006, GC007, GC010, GC011, GC016, GC017 and GC021 were found promising.
  M.A. Islam , M.A. Samad and M.B. Rahman
  The alum precipitated formalin killed fowl cholera vaccines (FCV) are locally produced from the Livestock Research Institute (LRI), Mohakhali, Dhaka and Bangladesh Agricultural University (BAU), Mymensingh which are being used to control fowl cholera in chickens and ducks in Bangladesh. Efficacy of these two vaccines has been evaluated mostly in chickens but reports on ducks are very limited. Four weeks old 50 Jinding breed of ducks were used to evaluate the efficacy and immune responses of fowl cholera vaccines during the period from October 2002 to March 2003. These 50 ducks were divided into three groups (A = 20, B = 20 & C = 10 ducks) and each duck of group A was inoculated with FCV (LRI) @ 0.5 ml subcutaneously at the age of 8 weeks, and each duck of group B received FCV (BAU) @ 1.0 ml intramuscularly at the age of 12 weeks as manufacturer instruction, whereas ducks of group C served as unvaccinated control. Each duck of group A and B was also injected booster dose after two weeks of primary vaccination with their respective FCV. Each duck of all the three groups (A, B and C) was challenged after two weeks of post-booster vaccination with 1.0 ml inoculum containing 5.4x106 CFU of virulent Pasteurella multocida intramuscularly. The results of challenged experiment revealed that one (5.0%) duck of group A, two (10.0%) ducks of group B died within 2 to 3 days of post-challenged, whereas 10 (100%) unvaccinated control ducks of group C died within 1 to 3 days of post-challenged. Therefore, the FCV® (LRI) conferred protection to 95% and FCV (BAU) conferred protection to 90% of vaccinated birds against challenged infection after two weeks of booster vaccination. The mean values of Total leukocytic count (TLC), Total serum protein (TSP) and Passive haemagglutination assay (PHA) antibody titre of ducks in both the groups A and B were found significantly (p < 0.01) increased at two weeks of post-primary and two weeks of post-booster vaccination, and also two weeks of post-challenged infection in comparison to the respective pre-vaccination values. These results indicate that the FCV of LRI induced comparatively higher TLC, TSP and PHA antibody titre than FCV of BAU in ducks. These results showed that the locally prepared fowl cholera vaccines induced sufficient cellular and humoral immune responses which resulted satisfactory level of protection against duck cholera and therefore both the locally prepared FCV could be recommended to control duck cholera under filed conditions in Bangladesh.
  M.M. Rahman , M.A. Islam , M.Y. Ali , M.E.A. Khondaker and M.M. Hossain
  A total of 21 four weeks old Nara males divided into 3 groups as A-non-caponized, B-chemically and C-surgically caponized birds having 7 males each. The birds were caponized at 4 weeks of age and reared on individual cages up to 16 weeks of age to assess the body weight gain, hematological traits and blood cholesterol concentration. The weight gain was significantly increased in caponized birds compared to non-caponized birds (p<0.01). However, surgically caponized birds gained the highest body weight. Total erythrocyte count, packed cell volume and hemoglobin concentration were significantly reduced in caponized birds compared to non-caponized birds (p<0.01). Testis weight of group A and B at 16 weeks of age were 19.20 and 2.58 g/bird respectively. The highest enlargement of liver and spleen was found in group-C followed by group-A and B. Adrenal gland weight of A, B and C were 0.14, 0.21 and 0.26g/bird respectively (p<0.01). Reduced comb, wattle and head of caponized birds were observed compared to the non-caponized birds. Caponization is the most suitable technique to have maximum meat from chicken. However, surgically caponization may be the best technique for maximum growth.
  M.A. Islam , M.M. Rahman , M.R. Islam and M.M. Khatun
  An attempt was undertaken for molecular characterization of infectious bursal disease virus (IBDV) field isolates. In order to isolate the virus, bursae of thirty five dead chicken with clinical infectious bursal disease (IBD) were collected from Bangladesh Agricultural University (BAU), Mymensingh. Isolation of field strain of IBDV was carried out in chickens of 5-week-old. Five IBDV isolates were obtained from chicken inoculation. Reverse transcriptase-polymerase chain reaction (RT-PCR) was preformed to detect IBDV isolates in the bursal tissues. RT-PCR couple with restriction enzyme (RE) analysis was carried out for molecular characterization of IBDV isolates to determine the pathotype. 677 bp fragments from IBDV genome segment A corresponding to the hyper variable domain of outer capsid protein VP2 was amplified by RT-PCR. Two restriction endonuclease (REs), SspI and SacI were used for digestion of RT-PCR products. RT-PCR product was digested by SspI but not SacI. The presence of SspI restriction site in the 677 bp RT-PCR fragment indicated that IBDV isolates belonging to very virulent (vv) pathotype.
  M.A. Islam , M.A. Samad , M.B. Rahman , M.T. Hossain and S. Akter
  The duck plague vaccine (DPV) is locally produced from the Livestock Research Institute (LRI), Mohakhali, Dhaka which is used to control the duck plague viral disease in ducks in Bangladesh. Efficacy of this vaccine reports has no, so to say on ducks in Bangladesh. Three weeks old 50 Khaki Cambell Ducks were used to evaluate the induced of immune responses of duck plague vaccine during the period from February to April 2003. These 50 ducks were divided into three groups (A = 15, B = 15 and C = 5 ducks) and each duck of group A and group B were inoculated primarily with duck plague vaccine @ 1.0 ml. intramuscularly at the age of 4 weeks and whereas ducks of group C served as unvaccinated control. Then each duck of group A was also injected booster dose after 2 weeks of primary vaccination with same vaccine, dose and route. The mean value of TLC, TSP and PHA antibody titre of ducks of group A was found significantly (p < 0.1) increased at two weeks and four weeks of post-primary vaccination and two weeks and four weeks of post-booster vaccination in comparison to the pre-vaccination values. The mean value of TLC, TSP and PHA titre of ducks of group B was also found significantly increased (p < 0.1) at two, four, six and eight weeks of post-primary vaccination in comparison to the pre-vaccination values. These results indicate that booster vaccination of duck plague vaccine induced comparatively higher TLC, TSP and PHA antibody titre than single primary vaccination in ducks. The mean value of TLC, TSP and PHA in unvaccinated control group C was more or less nearer at the age of four weeks and 12 weeks in ducks. These results showed that the locally prepared duck plague vaccine induced sufficient both cellular and humoral immune responses with booster-vaccination than primary vaccination in ducks. Therefore, it could be recommended to booster dose of vaccination of duck plague vaccine to control duck plague under field condition in Bangladesh.
  M.T. Hossain , M.A. Islam , M.M. Amin and M.A. Islam
  The comparative efficacy between the conventional vaccine (DLS-DPV) and experimentally prepared duck plague vaccine (BAU-DPV) was evaluated in seventy-five 35-day-old Zinding breed ducklings during the period from October/02 to March/03. The ducklings were equally divided into five groups (A, B, C, D and E). Ducklings of group A and B were primarily vaccinated with 0.5 ml and 1.0 ml of DLS-DPV respectively and those of group C and D were primarily vaccinated with 0.5 ml and 1.0 ml of BAU-DPV. The ducklings of group E were kept as unvaccinated control. Five months after primary vaccination all the ducks of vaccinated groups were boosted with 1.0 ml of DLS-DPV and BAU-DPV and 21 days after booster vaccination all the ducks of vaccinated and control groups were challenged with 1ml of 10 4 EID50 of virulent field isolate of duck plague virus (DPV). The level of immunity developed in different vaccinated groups of ducks was measured by passive hemagglutination (PHA) test. The mean PHA titre of birds of group A, B, C and D after primary vaccination were 38.4 ± 6.4, 28.8 ± 3.2, 51.2 ± 7.84 and 38.4 ± 6.4 and after booster vaccination were 153.6 ± 25.6, 76.8 ± 12.8, 358.4 ± 62.71 and 115.2 ± 12.8 respectively. Results of PHA test indicated that experimentally prepared duck plague (BAU-DPV) vaccine revealed higher immune response compared to that of the conventional (DLS-DPV) vaccine and results of the challenge experiment indicated that the mean PHA titre over 100 after booster vaccination revealed 100% protection.
  M. Asaduzzaman , M.S. Jahan , M.R. Mondol , M.A. Islam and A.K. Sarkar
  A total of ninety-six Shaver 579 pullets were fed on isocaloric and isonitrogenous diet with vitamin-mineral premixes, Supramixes Layer, Acimix Super-L and Adivit-L and without premix (control) in the age duration from 25 to 40 weeks to evaluate the efficacy of different commercial premixes. Daily feed intake and body weight gain of the pullets did not differ significantly (P>0.05). Hen day egg production of different treatments differed significantly (P< 0.01). Higher and statistically similar egg production was obtained in Supramix layer (75.4%) and Acimix Super-L (74.12%) treatment. The lowest (62.5%) and statistically similar (65.99%) egg production occurred in control and Adivit-L treatment respectively. Egg weight of the treatments differed significantly (P< 0.01). The highest (57.15g) and statistically similar (56.57g) egg weight was observed in supramix Layer and Acimix Super-L group respectively. The highest egg mass output of Supramix Layer (42.98) differed significantly (P< 0.01) from the control (33.98). Significant (P< 0.01) difference was observed in the FCR value of Supramix Layer (2.68) and control (3.43) group. Broken egg percent of the treatments differed significantly (P< 0.01). High broken egg (4.11%) was observed in control group. No significant (P>0.05) differences were observed in livability. The observation of the results showed that use of commercial vitamin-mineral premix to supplement the diet of caged laying pullets is inevitable to maintain desirable production level. But the efficacy of all commercial premixes was not equal. The quality of marketed premixes is not uniform which is revealed in terms of performance test in the study.
  A.J. Sikder , M.A. Islam , M.M. Rahman and M.B. Rahman
  The present study was undertaken to know the seroprevalence of Salmonella and Mycoplasma gallisepticum (MG) infection in six model breeder poultry farms (MBPFs) located at kalapara Upazilla under Patuakhali district, Bangladesh. A total of 364 sera samples were collected from chickens belonging to six MBPFs. All sera samples were examined by rapid serum plate agglutination (SPA) test using commercial Salmonella (SP) and MG antigens to determine the presence of Salmonella and MG specific antibodies in different age and sex of birds belonging to MBPFs. In addition to that prevalence of Salmonella and Mycoplasma infection in MBPFs during rainy and winter seasons were also recorded. The results of serological tests were analyzed statistically. The overall prevalence of Salmonella and Mycoplasma infection in six MBPFs were recorded as 23.46% and 46.88% respectively. Prevalence of salmonella was recorded highest in rainy season (25.00%) than the winter season (21.88). On the contrary, Mycoplasma infection was recorded highest in winter season (61.45%) than the rainy season (51.74%). Both Salmonella and Mycoplasma infections were recorded highest in female birds (24.10%) than the male birds (15.62%). The prevalence of MG infection decreased with the increase of age. MG infection recorded highest 71.42% at 18 weeks of age and lowest 50% at 22 weeks of age. On the other hand, the prevalence Salmonella infection was increased with the increase age. Salmonella infection was found highest 30.76% at 39 weeks of age and lowest 13.33% at 32 weeks of age. It was concluded from the present study that both Salmonella and MG infection were significantly present in all six MBPFs and SPA test could be used as a tool for quick detection of Salmonella and MG infection.
  B.M. Hasin , A.J.M. Ferdaus , M.A. Islam , M.J. Uddin and M.S. Islam
  The research was conducted with natural ingredients and orange skin in the diet of laying pullets to determine the suitability as pigmenting agents of egg yolk for laying chicken The influence of test ingredients on production characteristics of laying pullets, internal and external quality characteristics of eggs were investigated simultaneously. The experiment covered the proximate analysis of marigold, orange skin meal to determine the nutrient concentrations, xanthophylls contents and 7th-12th weeks feeding trail to investigate the yolk pigment availability of marigold and orange skin as well as laying performance of birds. Orange skin and marigold were used for comparing the pigmenting ability to normal feed containing xanthophylls. Yolk color was improved significantly as compared to control for addition of marigold and orange skin during 4th, 8th and 12th weeks of supplementation. Inclusion of marigold and orange skin in the diet of laying pullets did not cause deterioration in natural and external quality of eggs and there were no significant variations with respect to body weight, hen day egg production egg weight and feed conversion. There was no mortality of birds during study period. The results indicates the conclusion that between two natural ingredients marigold contain more xanthophylls (156.32mg/kg DM) than orange skin (83.02mg/kg DM) and use of 4% marigold meal in the diet of laying pullets is enough to produce eggs with yolk color score 11.00 close to 30 mg synthetic pigment/kg diet at the 12th week of supplementation, whereas the birds that received 4% orange skin in the diet were able to produce eggs with yolk color score 5.0. Moreover, use of marigold and orange skin in diet of pullet has no detrimental effect on internal and external quality of egg as well as egg production characteristics. So, marigold can be used more efficiently for egg yolk pigmentation.
  M.Y.A. Pramanik , M.A.R. Sarkar , M.A. Islam and M.A. Samad
  An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, during the period from May to November 1998 to investigate the effect of green manures and different levels of nitrogen on the yield and yield components of transplant aman rice (cv. BRRI Dhan32). The experiment comprised of 3 green manuring crops viz., Sesbania rostrata, Sesbania aculeata and Crotalaria juncea and 5 levels of N viz., 0, 20, 40, 60 and 80 kg ha-1. Among the green manuring crops Sesbania rostrata produced the highest grain yield of transplant aman rice compared to other green manuring crops. The highest grain yield was obtained when the crop was fertilized with 40 kg N ha-1 in combination with Sesbania rostrata. Sesbania aculeata exhibited similar behaviour as that of Sesbania rostrata but Crotalaria juncea showed lower performance in respect of yield and yield components of transplant aman rice. Sesbania rostrata also showed the best performance in respect of plant height, total number of tillers hill-1, number of effective tillers hill-1, number of grains panicle-1, weight of 1000 grains and straw yield compared to other green manures.
  M.A. Islam , M.S. Islam , M.A. Mazed and M.K. Uddin
  This experiment was conducted at Buffalo Breeding And Development Farm, Bagerhat to investigate conception rate, service per conception, gestation period, age at first calving, birth weight, postpartum heat period and calving interval of Nili-Ravi and crossbred buffaloes. It was revealed that average conception rate, service per conception, gestation period, age at first calving, birth weight, calving interval and postpartum heat period were 95.24 %, 88.46%; 1.05, 1.13; 301.486?6.72, 303.200?7.07 days; 61.857?3.44, 63.048?3.89 months; 33.266?3.49, 30.508?3.43 kg; 572.633?116.54, 581.481?94.15 days and 167.800?27.92, 174.500?41.04 days; respectively. It may be concluded that Nili-Ravi buffaloes were better than crossbred buffaloes.
  M.S. Islam , M.A. Mazed , M.A. Islam , M. A. Kadir and M. M. Rahman
  The study was conducted at two dairy farms namely, Government Dairy Farm, Faridpur and Central Cattle Breeding Station, Savar, Dhaka to investigate the effect of age of first service, conception rate, age at first calving, birtgh weight of calves and calving intervals on some reproductive parameters of Local X Freisian upgraded Dairy cows. From the study, it was revealed that average age at first service was 29.21?1.11 months. Means of the conception rate of different cows were 63.00?16.73 for artificial insemination and 85.00?17.17 for natural services. Average age at first calving was 39.30?4.53 months. Mean birth weight of calves of the representative cross bred was 15.32?0.78 kg. Average calving interval was 438.60?33.90, 435.75?32.35 and 424.37?38.66 days for first, second and third lactation, respectively. It may be concluded that the reproductive characters of Local X Friesian upgraded dairy cows are moderately standard and can be reared under Bangladeshi condition.
  M.A. Islam , M. A. Mazed , M. S. Islam and M.K. Uddin
  This experiment was conducted at Buffalo Breeding and Development Farm, `Bagerhat` a southern district of Bangladesh to investigate the milk (lactation) yield, lactation length and calf mortality rate of Nili-Ravi and crossbred buffaloes. From this study it was revealed that the average milk yield per lactation of Nili-Ravi and crossbred buffalo cows were 1161.805?684.07 and 696.636?258.68 liters respectively, while their average milk production per day were 3.5 and 2.24 liters. On the other hand average lactation length for same genotypes were 330.426?70.08 and 311.556?74.14 days respectively. The overall calf mortality rate was 24.28% and the average calf mortality rate for Nili-Ravi and crossbred buffalo were 37.1 and 14.1 percent respectively. Statistical analysis showed that there were significant variation (p<0.01) existed between Nili-Ravi and crossbred buffalo calves in respect of calf mortality. From the study it may be concluded that Nili-Ravi buffaloes are better than crossbred buffaloes considering specially on milk production mentioned in the Buffalo Breeding and Development Farm, Bagerhat, Bangladesh.
  M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman
  Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.
  D. Chandra and M.A. Islam
  Excised-leaf water loss was studied in 50 F5 bulk lines in five wheat crosses including their common parent. A wide range of variability for excised-leaf water loss recorded as 5.47 to 18.81% and 10.36 to 25.36% at 2 and 4 h after excision, respectively. Generally, the lines that higher water at 2 h after excision also losses higher at 4 h after excision. For excised-leaf water loss the heritability was very high ranging from 89.36 to 96.70% in the five crosses. The result suggested the involvement of additive genes in the control of excised-leaf water loss. In general a negative correlation was found between excised-leaf water loss and yield in all the crosses. Among the five crosses Kanchan x DSN-34 provided maximum high yielding lines with low excised-leaf water loss.
  M.R. Islam , S. Islam , M. Jahiruddin and M.A. Islam
  A pot culture experiment was carried out at Bangladesh Agricultural University (BAU), Mymensingh to see the effects irrigation water arsenic (As) on Boro rice (February to June) and the residual effect on T. Aman rice (August-November). There were eight treatments consisting of Control, 0.10, 0.25, 0.50, 0.75, 1.00, 1.50 and 2.00 ppm As added through irrigation water. A total of 56 L of irrigation water having different concentrations of As was needed for the Boro rice (Cv. BRRI dhan 29). After harvest of Boro rice, T. Aman rice (Cv. BRRI dhan 33) was grown in the same pots with monsoon rain. Nutrients such as N, P, K and S @ 100, 25, 40 and 25 ppm, respectively were added to sustain normal growth of both Boro and T. Aman rice. The irrigation water added As up to 0.25 ppm enhanced the plant height, panicle length, filled grains/panicle, 1000-grain weight and finally the grain yield of Boro rice and the further doses of depressed the plant growth, yield and yield components. The concentration of As in rice grain or straw of Boro rice increased significantly with increasing As concentrations in the irrigation water, the values for grain As for every As treatment were below the Maximum Permissible Level (1.0 ppm). Application of As added to the first crop (Boro rice) had significant residual effects on the second crop (T. Aman rice) in respect of plant height, panicle length, grains/panicle, grain and straw yields. Arsenic concentrations were always higher in Boro rice grain and straw compared to T. Aman rice. The grain As of Boro rice was almost double the As levels in T. Aman rice grain over the treatments. The As treatments had an adverse effect on the N, P, K and S concentration of rice grain.
  M.A. Bakar , M.A. Islam , S. Khatun and G. Sadik
  The sub-acute toxicity study of malic acid-propane 1,2-diol copolyester (MPC) was carried out in rats. The polymer was administered intraperitoneally to the rats at a dose of 300 F g rat G 1 day G 1 for 21 consecutive days. The gross general observations such as changes of body weight, hematological profiles, biochemical parameters of blood and the histopathology of liver, kidney, heart, lungs and spleen were investigated both in control and experimental rats. The body weights of the rats were slightly increased. The changes of hematological and biochemical parameters were statistically insignificant. No abnormalities were found in the histopathology of the liver, kidney, heart, lung and spleen in the experimental group of rats when compared with control group of rats. From this study, it was inferred that malic acid propane 1,2-diol copolyester would be used as an enteric coating material.
  M.R. Karim , M.A. Islam , N. Absar and F. Hashinaga
  A protease from tomato flesh was purified to homogeneity by (NH4)2SO4 precipitation followed by Sephadex G-75 and DEAE-cellulose column chromatography. Molecular weight of the enzyme was estimated to be 81-79 KD by gel filtration and SDS-PAGE respectively. The enzyme was found to be a single polypeptide chain as revealed by SDS-PAGE under either reducing or non-reducing conditions. Optimum activity was observed at pH 7.0 and 45°C with a km value of 0.48 per cent determined by using casein as substrate. The enzyme appears to be a serine protease being inhibited greatly by DFP and PMSF and to a lesser extent by heavy metals such as Pb2+ and Fe2+.
  M.M. Rahman , D.B. Biswas , M.M. Rahman , M.M. Islam and M.A. Islam
  Bacteriological examination of septic wounds in animals was conducted in the Veterinary Clinic, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period of January 2001 to July 2002. A total of 250 samples were collected for the identification of bacteria from the wound and 227 (90.8%) were found infected with bacteria. Staphylococcus aureus was the most common organism (33.92%) and then Escherichia coli (18.94%) and the lowest prevalence was the Streptococcus spp. (9.25%). In vitro drug sensitivity study revealed that Gentamycin, Cephalosporin, Neomycin and Streptomycin are potent antibiotic for the treatment of infected wound and Penicillin and Sulphamethaxazole are less sensitive to contaminated wound. For better conception and accurate treatment of complicated wounds, it is necessary to have cultural isolation of the microorganisms and antibiotic sensitivity of each isolate before the treatment.
  M.M.H. Khan , M.A. Islam , M.M. Hossain , M.M. Rahman and M.N. Islam
  Milk samples from single, twin and triplet or 1st, 2nd and 3rd lactation bearing Black Bengal goat were collected to monitor the physical (organoleptic, specific gravity and pH value) and chemical (acidity, fat, SNF, total solids, protein, lactose and ash content of milk) quality of milk. From the above tests, it was observed that the color, flavor, taste and texture of milk samples were normal; yellowish white, normal (goaty), slightly sweet and free flowing liquid. Except acidity and protein content, all other chemical traits varied significantly among different kid bearing goats milk. Chemical parameters were similar in different lactations except fat and total solids percentage. Results of the above experiments indicated that milk obtained from single kid bearing and 2nd lactating goats were better than the other number of kids bearing or lactating goats
  Hossain M.A. , M.M. Hossain , A.K. Azad and M.A. Islam
  The nature and extent of damage of shoot borer, Hypsipyla robusta (Moore) Lepidoptera: Pyralidae was studied in 2-8 years old Chukrasia velutina Wight et Arn. plantations in hilly areas of Chittagong, Bangladesh. A single tree could have attacked several times in the season. About 47% C. velutina trees were found to have attacked at least once in their lifetime. The infestation rate was more pronounced on the hill slopes (56%) than on the valleys (37%). A good number of trees (60%) were found to exhibit recovery trend after the infestation while other trees were with the formation of fork, cork, knot, curve, twist, whorled branching from the point of infestation (Mostly at a height of 2-3 m). It was estimated that about 42% potential biomass production was lost due to the infestation. Apart from that, some trees were found to show an encouraging `resistance` character keeping intact themselves against the infestation that referred an ample scope of propagation and plantation in future for a better inheritance.
  D. Chandra , M.A. Islam and N.C.D. Barma
  Variability, heritability, genetic advance and association of nine characters including yield in fifty F5 bulk lines of five wheat crosses viz. Kanchan×DSN-34 (1), Kanchan×YC-17 (2), Kanchan×YC-16 (3), Kanchan×BW-115 (4) and Kanchan×Ad-119 (5) were studied. All the characters studied e.g. days to anthesis, grain filling duration, spikes plant-1, plant height, grains spike 1, 100-grain weight, biomass plant-1, harvest index and yield plant-1 showed significant variation in all crosses. However, the expression of the traits in all crosses was not similar. Very high heritability and moderate genetic advance for days to anthesis and biomass plant-1 in crosses 1, 2 and 3 suggested the predominance of additive genetic variation for this two traits. Similar expression was observed for grain filling duration in crosses 1 and 2. Very high heritability and moderate to high genetic advance were observed for spike plant-1 in crosses 2, 4 and 5; plant height in crosses 1, 2 and 4; harvest index in crosses 2 and 3; grains spike 1 and yield plant-1 in all crosses. The heritability for 100-grain weight however, was moderate to high with low to very low genetic advance. Days to anthesis showed negative association with grain filling duration in crosses 1 and 3 and significant positive correlation with plant height, biomass plant-1 and yield plant-1 in cross 1 and with grains spike 1 in crosses 1 and 4. Grain filling duration showed mostly significant or non-significant negative correlation with all the traits with one exception where it showed significant positive correlation with 100-grain weight in cross 4. Spikes plant-1 and plant height showed significant positive correlation with biomass plant-1 and yield plant-1 in most crosses with few exception. The trait 100-grain weight did not show any significant correlation with any trait except in one occasion. Biomass plant-1 and harvest index showed significant positive correlation with yield plant-1 in most crosses, however, they did not show any significant correlation between biomass and harvest index.
  M.A. Mazed , M.S. Islam , M.M. Rahman , M.A. Islam and M.A. Kadir
  In the present study urea molasses multinutrient blocks (UMMB) may be used as a catalytic feeding to the straw based diet of the indigenous cows for fertility. With that understanding, 49 indigenous post partum cows from 49 marginal village farmers of village-Boira were selected and a cake of 250 g/h/d UMMB was supplied with their usual feeding from date to calving to the date of confirmation of pregnancy. Cows were stall fed. Reproductive events of cows were recorded as when occurred. Milk progesterone was measured by using radio immuno assay (RIA) technique. Similarly, 49 cows were in control group in the same village. The results in control and UMMB group were-calving to 1st p4 (progesterone) rise (days) 104±52 and 103±37 (p>0.05); calving to first service (days) 194±68 and 130±64 (p<0.01); calving to conception (days) 199±72 and 162±73 (p>0.05) and subsequent calving interval (days) 480±73 and 443±70 (p>0.05), respectively. From the study, it may be concluded that UMMB has a positive effect on fertility of the local cows under the village condition of Bangladesh.
  M.A. Samad , M.A. Islam and M.A. Khaleque
  An attempt was made to determine the effect of stocking densities on the growth of Magur (Clarias batrachus) fry (5.1 ± 0.10 mg) for a period of 28 days in trays in laboratory. Clarias batrachus fry were stocked at the rate of 30, 40, 50 and 60 fry/tray i.e. 3, 4, 5 and 6 fry/litre of water. Stocking density of 30, 40, 50 and 60 fry/trays were designated as treatment I, II, III and IV respectively the fry was feed by grounded tubifex. The survival rate of C batrachus fry upto 28 days were found 92.3, 90.3, 89.3 and 80.1% at the stocking density of 30, 40, 50 and 60 fry/tray, respectively. The treatment had no significant effects on the ADG, SGR and weekly growth increment in length. Average daily gain of Clarias batrachus was found 20.80, 21.80, 19.46, 19.31 in treatment I, II, III and IV. The value of SGR (%) was 17.58, 18.68, 17.33 and 17.30% in the above treatments. The final cumulative gain in length were 39.50 ± 0.51, 39.90 ± 0.10, 38.60 ± 0.60 and 37.60 ± 1.20 (mm) and fry reached to 588.3 ± 33.29, 604.3 ± 16.01, 550.0 ± 25.00, 546.6 ± 28.00 mg over a period of 28 days in treatment I, II, III and IV, respectively. The second experiment was performed to determine the effects of two selected feeds on the growth and survival rate C. batrachus fry in different nursery ponds for a period of 28 days. The protein level of the supplemental diet was 32.24% in feed I and 27.75% in feed II which were designated as treatment I and II. The feed was supplied daily at the rate of 10% of the body weight of the stocked C. batrachus fry. C. batrachus having an average initial length of 3.86 ± 0.05 cm reached to the final length of 7.26 ± 0.05, 7.12 ± 0.02 and in the gain in body weight and 3.38 ± 0.02 g under feeding treatment I and II, respectively. Analysis of variance (ANOVA) indicated that a significant difference (p>0.05) was observed in case of ADG, SGR, survival rate, cumulative growth increment in length and weight.
  M.R. Mondol , S. Dewan , M.A Hossain , M. Asaduzzaman , M.A. Islam and U.A. Rozario
  In the present experiment the fingerlings of Puntius gonionotus (Thai Sarpunti) were stocked in the experimental rice field plot of Agronomy Field Laboratory, Bangladesh Agricultural University, to study their food and feeding habits in rice field. The fingerlings of this fish were stocked after 20 days of transplanting rice seedlings and the samplings of fishes were done once in every month from June to August. The gut contents of fishes were analysed by the numerical method and percentage of frequency of occurrence method. The water quality parameters such as temperature, Dissolved Oxygen (DO), pH, total alkalinity, chlorophyll-a, nitrate-nitrogen and phosphate-phosphorus showed monthly variations and they were within productive range. A total of 37 genera of phytoplankton belonging to Chlorophyceae, Euglenophyceae, Cyanophyceae and Bacillariophyceae were recorded in the water of the experimental plot of rice fields and in zooplankton population a total of 13 genera belonging to Rotifera, Cladocera and Copepoda were recorded. Among the 4 groups of phytoplankton Chlorophyceae was the most dominant group and Euglenophyceae was the least dominant group and in zooplankton Rotifera was recorded as the most dominant group and Cladocera as the least dominant group. However among the plankton population, phytoplankton was recorded highly dominant over zooplankton in the water of rice field. Among the genera of phytoplankton Fragilaria, Navicula, Surirella, Chlorella, Chrysococcus, Scenedesmus, Ulothrix, Euglena, Cyclotella and Oscillatoria and among the genera of zooplankton Brachionus, Cyclops, Asplanchna and Nauplius were dominant in water of the plot. The total number of genera of phytoplankton recorded in the gut contents of P. gonionotus were 36 and that of the genera of zooplankton recororded were 6. Among the 4 groups of phytoplankton Bacillariaophyceae was the most dominant and preferred group. With respect to zooplankton. P. gonionotus showed very less preferance on them. Among the genera of phytoplankton the relatively more preferred genera were Navicula, Closterium, Fragilaria, Chrysococcus, Oscillatoria, Tabellaria, Scenedesmus, Ulthrix and Aphanizomenon. P. gonionotus showed very less preference on the genera of zooplankton. P. gonionotus may be regarded as planktivores feeding mostly on phytoplankton.
  M.H. Morshed , M. Shamim Hossain , M. Ibrahim , M.Z. Shafique , M.O.H. Helali , A. Samad , M.S. Islam and M.A. Islam
  The aim of the present study was to evaluate the antibacterial characteristic of the four synthetic plant hormones Indoleacetic acid (IAA), 2,4- dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA) and Gibberellic acid (GA). Antibacterial potency was assessed by measuring the zone of inhibitions on semi-solid nutrient agar bacterial inoculating petri dishes. NAA and IAA gave potent antibacterial activity at concentration of 150 μg disc-1 giving zones at 26-35 mm. Bacteriostatic and bactericidal concentrations against the pathogens were determined by serial dilution technique. Bacteriocidal concentrations of the tested principles were found to be significantly higher than their respective bacteriostatic concentrations. Minimum inhibitory concentrations that are bacteriostatic concentrations of the tested compounds were found at 32-128 μg mL-1 whereas, bactericidal concentrations established at 256-512 μg mL-1 against eight pathogenic bacteria Streptococcus -haemolyticus, Staphylococcus aureus, Pseudomonas aeroginosa, Shigella dysenteriae, Shigella flexneri, E. coli, Salmonella typhi and Klebsiella pneumoniae.
  M.M. Haque , N.T. Narejo , M.A. Salam , S.M. Rahmatullah and M.A. Islam
  A four month long experiment was conducted in earthen ponds (each of 81 m2 in size) during the month from August to November, 2001 to observe suitable stocking density of Macrobrachium rosenbergii in polyculture of Indian major carps viz. catla (Catla catla), ruhu (Labeo rohita) and mrigal (Cirrhinus mrigala). Six densities of M. rosenbergii with carps were considered as treatments. Each treatment had three replications. All the fish were same age group and mean initial weight of prawn, catla, ruhu and mrigal were 1.1± 0.12, 14.2 ± 0.52, 8.5 ± 0.16 and 11.8 ± 0.20g respectively. Water quality parameters including temperature, dissolved oxygen and pH were found in the suitable range. The best combination of prawn-carp polyculture (prawn: catla: ruhu: mrigal = 25: 15: 15: 15) was found in the treatment T3 with significantly (p<0.05) highest growth of M. rosenbergii and carps.
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