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Articles by M.A. Fadol
Total Records ( 5 ) for M.A. Fadol
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nourand and M.A. Fadol
  An anthrax and sheep pox combined vaccine was made in a lyophilized form by mixing concentrated live spores of Bacillus anthracis (Sterne strain) of known count with sheep pox virus (0240 vaccine strain) of a known titer. It induced protection against anthrax in guinea pigs and sheep pox diseases in sheep after challenge with virulent Bacillus anthracis and sheep pox virus respectively. The vaccine was found stable after 15 month of preservation at -20C.
  Amira M. Elhassan , M.A. Fadol and A.E. Karrar
  During this study swab samples were tested for demonstration of Infectious Bovine Rhinotracheitis/ Infectious Pustular Vulvovaginitis (IBR/IPV) virus (BHV-1) from different regions of the Sudan. Samples were collected from cases suffering from infertility, nasal discharges, vaginal discharges, lacrimation and abortion. The virus was isolated from five cases with typical signs of the disease including two from Niyala, two from Atbara and one from West Kordofan. Also using SNT, ELISA and PHA testes neutralizing antibodies were found, which confirmed the presence of the disease.
  Amira M. Elhassan , M.A. Fadol and A.E. Karrar A.R.M. Elhussien
  During serosurveillance to detect infectious bovine rhinotraceitis antibodies, 856 serum samples were collected from different states of the Sudan. The overall percentage of positive samples was 47.43% (406 samples) tested by PHA. Of 273 samples tested by ELISA 141 (51.65%) were positive, whereas 69 out of 94 samples tested by SNT 73% were positive. The prevalence was found high in states including Jongeli, West and North Kordofan. In comparison between the three serological tests, SNT is recommended to be the technique of choice followed by ELISA, while PHA is a good technique to define the disease.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ELHussein , Tageldin A.M. Nour and M.A. Fadol
  The combined anthrax and sheep pox vaccine was prepared in a lyophilized form containing the recommended doses of both sheep and goat strain 0240 pox and anthrax live spore vaccine. Immunization of sheep with this vaccine revealed a considerable immune response when detected with immunocapture Enzyme Linked Immunosorbent Assay (ELISA), indirect ELISA and with (3-(4, 5-dimethylthiazolyl-2) -2, 5-diphenyltetrazolium bromide) (MTT) lymphocytes proliferation assay. The difference in titers of antibodies raised against capripox virus antigen was significant, it was also significant for antibodies raised against crude Protective Antigen (cPA) and killed spore antigen. Phytohemagglutinin (PHA) revealed high Stimulation Index (SI) before vaccination but low one after vaccination while the SPPV antigen showed slight SI before vaccination and high SI after vaccination indicating cellular immune response to this antigen. Low SI of PHA after vaccination might be due antimitogenic effect of SPV antigens.
  Abbas Mohamed Ahmed , M.M. Mukhtar , A.M. ElHussein , Tageldin A.M. Nour and M.A. Fadol
  The immune response of Sudanese sheep vaccinated with capripox vaccine was detected by IFA, Ic-ELISA test and MTT lymphocytes proliferation assays. The difference between antibodies titers before and after vaccination was found significant when tested by IFA test and the difference between the mean OD values was also significant when Ic-ELISA was used. PHA was found more effective in stimulating peripheral blood lymphocytes before vaccination compared with the virus antigen. After vaccination, the mean stimulation index of the of the virus antigen was higher
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