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Articles by M.A. Bari
Total Records ( 15 ) for M.A. Bari
  R.S. Sultana , M.A. Bari , M.H. Rahman , M.M. Rahman , N.A. Siddique and N. Khatun
  Plantlets regeneration were achieved in callus culture of leaf segments from five days old field grown seedlings of Citrulus lanatus Thumb. Callus induction and plant regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in MS medium supplemented with 2.5 mg L-1 2, 4-D. After transfer of this callus on MS medium supplemented with 1.0 mg L-1 BA+0.2 mg L-1 NAA induce adventitious shoots and developed into plant by further subculture in the same medium. NAA (0.1 mg L-1) was found effective in the production of root. Plantlets were acclimatized and subsequently transferred to the field. Survival of the plantlets under ex vitro condition was 80%.
  M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman
  Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.
  N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah
  The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.
  N.A. Siddique , M.A. Bari , N. Khatun , M. Rahman , M.H. Rahman and S. Huda
  Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  N.A. Siddique , M.A. Bari , A.T.M. Naderuzzaman , N. Khatun and M.H. Rahman
  This paper deals with over one hundred plants of ethnobotanical importance, used by the village doctors especially village kaviraj of Barind Tract for medicinal purposes.This work aims at the preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines and also identification of endangered medicinal plants by questionnaire survey.The data presented in this paper has been collected by personal contact with the local people and herbal practitioners, obtaining the information with great difficulty because of their recticence in divulging the secret of the identity of plants of great traditional reputation.
  N.A. Siddique , M.A. Bari , Sharmin Shahnewaz , M.H. Rahman , M.R. Hasan , M.S.I. Khan and M.S. Islam
  Callus cultures were initiated from nodal segments on Murashige and Skoog[19] medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  M.A. Awal , Ainun Nahar , M. Shamim Hossain , M.A. Bari , M. Rahman and M.E. Haque
  Present study assayed the toxicity effect of ethanolic leaf and seed extract of Cassia alata and found promising activity. From the probit transformation of resulting mortality data we got LC50 values of 4.31 ppm (μg mL-1) for seed and 5.29 ppm for leaf extract. Seed extract explored potent cytotoxicity similar to the standard gallic acid (LC50 = 4.53 ppm). From the antibacterial and MIC data it was evident that seed extract gave least activity against the tested bacteria whereas leaf extract deserved promising antibacterial activity.
  N.A. Siddique and M.A. Bari
  Callus cultures were obtained from axillary shoots of Hemidesmus indicus on (MS) medium supplemented with 2,4-D, NAA, IAA and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg L-1 NAA and 2.0 mg L-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (90.00%) from axillary shoot derived callus on MS medium containing 1.0 mg L-1 NAA and 2.5 mg L-1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  N.A. Siddique , Jaime A. Teixeira da Silva and M.A. Bari
  Folk medicines are complemented with other systems of traditional medicines. An account of about 200 hundred plants used in medicine among certain herbal doctors of Bangladesh is given; details of local name, scientific name, method of application or administration and dosage are given. These records include several uses which do not seem to be known or recorded in literature. It is discussed that indigenous knowledge assists in the wider application and acceptance of traditional medicine. This study makes it amply clear that the wild medicinal plants in Bangladesh folk life are used to meet all kinds of requirements. Our grandfathers and grandmothers are the carrier of indigenous knowledge regarding medicinal plants but today we are not conserving this knowledge due to decrease indigenous people. So it is now important to preserve this indigenous knowledge.
  N.A. Siddique , M.H. Kabir and M.A. Bari
  The experiment was conducted at Plant Biotechnology Laboratoty of Institute of Biological Sciences, Rajshahi University, Bangladesh. Subsequent regeneration performance of two medicinal plants (Aristolochia indica Linn and Hemidesmus indicus R.B were studied throughout the experimental period. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction, Callus regeneration and root induction. Among the different concentrations and combinations of growth regulators, the highest percentage of callus induction was 90.00% on MS medium supplemented with 1.0 mg L-1 NAA and 1.0 mg L-1 BAP from Aristolochia indica where as 95.00% on MS medium supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 Kn from Hemidesmus indicus. The highest percentage (85.00) of shoot regeneration was obtained in MS medium fortified with 1.0 mg L-1 BAP and 2.5 mg L-1 NAA from Aristolochia indica where as 95.00% on MS medium supplemented with 2.5 mg L-1 Kn and 1.0 mg L-1 NAA from Hemidesmus indicus. Regenerated shoots rooted best on MS medium containing 2.5 mg L-1 Kn and 2.0 mg L-1 IBA. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
  Sharmin Shahnewaz , M.A. Bari , N.A. Siddique and M.H. Rahman
  The present studies were carried out to evaluate the response of anthers of six rice genotypes to callus induction and plant regeneration. For callus induction Z2 media supplemented with 2, 4-D 0.5 mg l-1, NAA 2.5 mg l-1 and Kinetin 0.5 mg l- was used. Of the six genotypes, five gave good callusing response. Highest callus induction was observed in BRRI Dhan-29 (8.06%) and lowest in BR-10 (1.42%). Callus induction frequency varied from 1.42-8.06% depending on genotypes. Modified MS medium supplemented with Kinetin 0.5 mg l-1, BAP 2 mg l-1 and NAA 1.0 mg l-1 was used for plant regeneration. The results showed a significant genotypic difference in callus induction and plant regeneration. The regeneration of plantlet from anther derived calli ranged from 57-75% conclusion.
  A.N.K. Mamun , M.N. Matin , M.A. Bari , N.A. Siddique , R.S. Sultana , M.H. Rahman and A.S.M. Musa
  The woody legume, Albizia lebbeck, commonly cultivated for social forestry in rural area of Bangladesh was selected for micropropagation by tissue culture technique. Cotyledon, nodal segment of in vitro grown seedlings and nodal segments of field grown mature tree were used as explants. With proper manipulation of cytokinin and auxin combinations and concentrations, it was possible to induce callus from three types of explants of Albizia lebbeck. Different combinations of BA with NAA and KIN with NAA were proved efficient media formulation for callus induction. Among all the hormonal supplements used BA-NAA combination with MS medium was proved best in all respect of callusing response in Albizia lebbeck. Among the explants the in vitro internodal segments were the best for callus induction. The highest 100% callus induction was observed in media having MS+2.0 mg L-1 BA+0.2 mg L-1 NAA and the fresh weight of callus was 1.3 g. Among these three explants of Albizia lebbeck only calli derived from cotyledon regenerated best shoots in MS media supplemented with BA singly or in combination with NAA and KIN. In respect of direct shoot regeneration nodal explants of Albizia lebbeck produced highest range of regenerated shoots.
  M.Z. Rahman , M.H. Rahman , M.U. Mullah , N. Nahar , R.S. Sultana , M.A. Bari and M. Hossain
  The effects of various cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (CW) alone or in combinations on shoot multiplication of banana cv. Anupom were investigated. The rate of multiplication varied in different treatments. Among the treatments, MS medium supplemented with 5.0 mg L-1 each of BAP + KIN and 13% coconut water produced the highest number of shoot per explant (5.8±0.154). The number of shoots responded for rooting and their survivability were found higher with IBA than NAA and IAA. MS medium supplemented with 1.0 mg L-1 IBA produced the highest number of roots per shoot (7.±0.245). Plantlets grown without any auxin in the medium gave the least number of roots/shoot (2.60±0.219). The rooted plantlets were successfully transferred in the polybags and finally were well established in the field.
  N.A. Siddique , M.A. Bari , M.M. Pervin , N. Nahar , L.A. Banu , K.K. Paul , M.H. Kabir , A.K.M.N. Huda , M.U. Mollah and K.M.K.B. Ferdaus
  This study deals with over two hundred medicinal plants of ethnobotanical importance, used by the village doctors especially village Kavirajs in Barind Tract for medicinal purposes. This study aims at the identification of endangered medicinal plants by questionnaire survey and also preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines.
  N.A. Siddique , M.A. Bari , M.M. Pervin , N. Nahar , L.A. Banu , K.K. Paul , M.H. Kabir , A.K.M.N. Huda , K.M.K.B. Ferdaus and M.J. Hossin
  A procedure for rapid in vitro propagation of the aromatic and medicinal plant Aristolochia indica Linn. (Family: Aristolochiaceae) from axillary shoots is described. The highest percentage of callus induction was (95.00) on Murashige and Skoog (15) (MS) medium supplemented with 2.0 mg L-1 Kn and 1.0 mg L-1 BAP. Colour of the calli were mostly light green to dark green. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented with BAP and Kn alone or in BAP combination with NAA and IAA or NAA, IAA and BAP in combination with Kn. The Highest percentage (95.00) of shoot regeneration was obtained in MS medium fortified with 2.5 mg L-1 Kn and 1.0 mg L-1 BAP. The elongated shoots developed roots on a medium containing 1 mg L-1 Kn. The rooted plants were transferred to soil.
 
 
 
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