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Articles by M.A. Bari Miah
Total Records ( 3 ) for M.A. Bari Miah
  M.E. Haque , M.M. Pervin , M.Z. Rahman , K.M.K.B. Ferdaus , M.H. Kabir , M.A. Rahman , M.A. Bari Miah , M.R. Islam and M.T. Hoque
  The aim of present study was to investigate the bioactivity (antibacterial, antifungal and cytotoxicity etc.) of the ethylacetate extract isolated from Streptomyces sp. Aspergillus sp. and Bacillus sp. from sewage of different regions of Bangladesh. The ethylacetate extract of Streptomyces sp., Aspergillus sp. and Bacillus sp. shown modest antibacterial and antifungal activities at the concentration of 200 μg disc-1. The maximum antibacterial and antifungal activities were shown by the Streptomyces sp. The minimum inhibitory concentration of the microbial extracts was between 64 to 128 μg mL-1 against test organisms. Brine srimp lethality bioassay was carried out for cytotoxicity measurement of the extracts and the LC50 values were calculated after probit transformation of the resulting mortality data. All the ethyl acetate extract showed lower cytotoxicity properties (Streptomyces sp. 42.37 μg mL-1, Aspergillus sp. 52.25 μg mL-1 and Bacillus sp. 47.51 μg mL-1) compared with the reference standard Bleomycin (0.41 μg mL-1) and Galic acid (4.53 μg mL-1).
  R.S. Sultana and M.A. Bari Miah
  An efficient protocol was developed for direct plant regeneration, multiplication and rooting in in vitro condition of Momordica charantea Linn. locally known as "karalla". Nodal segments and shoot tips from in vitro grown seedling were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BA, KIN) and auxins (NAA, IAA) for direct shoot regeneration. Best response towards multiple shoot regeneration was obtained from the nodal segments of Momordica charantea Linn. on MS medium supplemented with 2.0 mg l G 1 BA + 0.2 mg l G 1 NAA. In this combination, the frequency of multiple shoot regeneration response from nodal segments was 95% and average number of shoots per explant was 8.20 " 0.26. The length of the longest shoot was 5.20 " 0.21 for nodal segments cultured on same combination. Efficient rooting was achieved on half strength of MS + 0.5 mg l G 1 NAA. About 90% of the regenerated plantlet survived in the field condition.
  R.S. Sultana , M.A. Bari Miah , M.M. Rahman , M.H. Rahman and M.U. Mollah
  Effects of different concentrations of sucrose, agar and different levels of pH on in vitro axillary shoot multiplication of Momordica charantea Linn. were studied in the present investigation. The nodal segments from the field grown plant were used as testing plant material. For maximum number of shoot induction and multiplication in MS medium containing 2.0 mg L 1 BAP+0.2 mg L 1 NAA with 30 g L 1 of sucrose, 7 g L 1 of agar and 5.5-6.0 levels of pH proved more effective. When the medium having 30 g L 1 sucrose, the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for total number of shoot per culture and average length of shoots and that were 5.10.8 and 5.60.4 cm, respectively. The highest response of shoot proliferation from the nodal explant was observed on MS medium having 7 g L 1 of agar and the frequency was 100%. Among different levels of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5-6.0 levels. It was proved by the present investigation that in vitro growth and shoot multiplication was affected by sucrose, agar and pH on the shoot induction medium.
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