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Articles by M. Wang
Total Records ( 2 ) for M. Wang
  W.J. Liu , Y. Fang , G.X. Fang , M. Wang , H. Yu , X.L. Li , T.T. Feng , H. Chen and S. G. Yu
  In the present study, the keratins and Keratin-Associated Proteins (KAPs) are one of the largest gene families in mammalian genomes encode, which is a heterogeneous group of proteins that make up about 90% of the cashmere fiber. Also, it regarded as a candidate gene of cashmere production traits. In this study, we aimed to detect polymorphisms of KAP16.6 gene and to investigated their associations with cashmere production traits (fiber diameter, cashmere yield, down cashmere thickness, body weight after combed cashmere) of three local goat breeds in China. In Xinjiang goat, statistical evaluation revealed significant differences (p<0.05) between the fiber diameter and cashmere yield trait of GG genotype. In Nanjiang cashmere goat, it is no significant differences (p>0.05) between cashmere production traits. The missense mutation of KAP16.6 gene in 816 cashmere goat samples was firstly detected in three of Xinjiang local goat breeds. Also, parts of these samples were sequenced. The results showed that frequencies of the KAP16.6-G allele in Xinjiang goat (n = 220), Nanjiang cashmere goat (n = 310) and BoGeDa cashmere goat breeds (n = 286) were 0.705, 0.603 and 0.600, respectively. The χ2 test showed that the genotype distributions in these three cashmere goat breeds were in agreement with Hardy-Weinberg equilibrium. According to the classification of PIC, BoGeDa cashmere goat was more polymorphic at this locus. Then a missense mutation was described at KAP16.6 locus in Xinjiang local goat breeds for the first time. The results possibly revealed that the size polymorphism existed in the three Xinjiang local goat breeds.
  X.M. Song , S.S. Lu , M. Wang , Q.Y. Li , D. Li , X.G. Yang , Y.Q. Lu , M. Zhang and K.H. Lu
  This study evaluated the effects of Glutathione (GSH) supplemented in the semen extender during the buffalo sperm sorting process on sperm quality, embryonic development after IVF and pregnancy rate after AI. The percentage of sperm motility, plasma membrane integrity and DNA fragmentation were detected by flow cytometry or by microscopy in stained, sorted and frozen semen treated with or without 0.75 mM GSH during the flow sorting procedure. The cleavage and blastocyst rates were examined at day 2 and 6-8 after IVF with frozen semen treated with or without 0.75 mM GSH. Pregnancy diagnosis was determined by transrectal palpation at 90 day after AI with frozen semen treated with or without 0.75 mM GSH. The percentage of sperm with Progressive Motility (MP, %) was significantly higher (p<0.05) in sorted semen supplemented with 0.75 mM GSH than that in the control. The percentages of moribund, dead and Phosphatidylserine (PS) translocated sperm detected by flow cytometry were significantly decreased (p<0.05) in frozen semen supplemented with GSH compared to the control. Higher blastocyst and pregnancy rates (p<0.05) were found after IVF and AI with frozen sperm treated with 0.75 mM GSH than that in the control group. In conclusion, addition of 0.75 mM GSH to the semen extenders (stained, sorted and frozen) during the sperm sorting process can improve sperm quality in vitro embryonic development and in vivo fertility after AI thus indicating potential for commercial application in buffalo sperm sorting.
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