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Articles by M. Tripathi
Total Records ( 3 ) for M. Tripathi
  P. Pandey , Rajesh Kumar , V.R. Pandey , K.K. Jaiswal and M. Tripathi
  Heterosis is the most important phenomenon for breaking the yield barrier level of crops. Hence, an experiment was undertaken to identify the best heterotic combinations for exploitation of heterosis or hybrid vigour. In this context, three cytoplasmic male sterile lines were crossed with twenty genotypes of pigeonpea in a linextester mating design during Kharif 2011-12. Thus, the resultant 60 hybrids along with their parents and standard check variety (NDA 2) were evaluated in a randomized block design with three replications during Kharif 2012-13. The results indicated that the manifestation of heterobeltiosis for seed yield per plant was significantly superior of fourteen hybrids ranging from -85.06-33.74% and fifteen hybrids over standard variety ranging from -82.57-26.28%. Most of the crosses which exhibited superiority over better parent or standard variety for seed yield also showed significant heterosis for primary branches per plant, secondary branches per plant, pods per plant, seeds per pod, biological yield per plant and harvest index. Besides seed yield, substantial heterosis was also observed in negative as well as positive direction for remaining characters but number of crosses showing significant estimates of heterosis and its degree varied from one character to another. The best cross combinations in order of merit seed yield and other yield components were NDACMS 1-6AxNDA 98-6, NDACMS 1-6AxNDA 5-14, NDACMS 1-4AxIPA 208, NDACMS 1-6AxICP 870, NDACMS 1-6AxNDA 96-1, NDACMS 1-6AxNDA 8-6, NDACMS 1-6AxICP 2309 and NDACMS 1-4AxBahar. Commencing the experimental findings it could be accomplished that the crosses, NDACMS 1-6AxNDA 98-6, NDACMS 1-6AxNDA 5-14, NDACMS 1-4AxIPA 208, NDACMS 1-6AxICP 870 were found to be more than 20% standard heterosis for seed yield recommended for commercial utilization.
  M. Tripathi and S.K. Garg
  Bioremediation is being viewed as a clean technology for decontamination of pentachlorophenol and chromium from tannery effluent. This study was conducted to isolate an efficient bacterial culture from treated tannery effluent which is tolerant to pentachlorophenol and Cr (VI) and could be employed for simultaneous bioremediation of both the toxic contaminants. Tannery effluent sample was collected from Common Effluent Treatment Plant, Kanpur (India). Fifty four bacterial cultures isolated by enrichment culture technique were screened for PCP and Cr (VI) tolerance on minimal salt medium supplemented with glucose as a cometabolite. The isolate B4 was found to be maximally tolerant to high concentration of both pentachlorophenol (500 mg L-1) and chromium (VI) (200 mg L-1) and was selected for further studies. It was identified as Bacillus sp. by morphological and biochemical analyses. The effect of various growth parameters such as carbon source at 0.2-1.0% (glucose, maltose, sucrose), pH (6.5-8.0), temperature (25-40°C) and inoculum size (0.5-2.5% v/v) were evaluated in minimal salt medium supplemented with 500 mg L-1 PCP and 200 mg L-1 Cr (VI). The best growth was exhibited at 0.4 % glucose, pH 7.0, 35°C and with 1.0% inoculum under shaking at 150 rpm. Thus, our isolate appears to have great potential for simultaneous bioremediation of pentachlorophenol and hexavalent chromium from the contaminated sites.
  Satyendra Kumar Garg , M. Tripathi and N. Lal
  In the present study, different approaches are being compared for the decolorization of reactive orange 4 monoazo dye by Bacillus cereus isolate under varied cultural and nutritional conditions. By employing conventional one-factor-at-a-time approach, the bacterial strain exhibited decolorization activity over a wide range of pH (7.0-9.0), temperature (30-38°C), dye concentration (50-200 mg L–1 MSM) and inoculum size (1.0-6.0%, v/v), with peak activity (68.2% color removal) at pH 8.0, 35°C, 50 mg dye L–1 MSM and 4.0% (v/v) inoculum in the presence of 1.0% (w/v) glucose as carbon/energy source and 0.2% (w/v) ammonium nitrate within 72 h incubation. Under response surface methodology (RSM using Box-Behnken design) approach, the dye decolorization enhanced to 100% at optimized 40 mg reactive orange L–1 MSM, glucose 1.0% (w/v) and ammonium nitrate 0.2% (w/v) during 72 h of incubation. The dye decolorization time was advanced by 12 h in bioreactor trial and 100% color removal was achieved within only 60 h incubation. In future experimentation, we envisage to test the potential of our isolate for the decolorization of other variety of azo dyes, mixture of dyes as well as the real textile effluent.
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