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Articles by M. Solouki
Total Records ( 2 ) for M. Solouki
  A.A. Vahabi , A. Lotfi , M. Solouki and S. Bahrami
  In this study, molecular and morphological variation of 22 population of Plantago ovata assessed using RAPD, ISJ and agro-botanical markers. Field experiment was conducted in completely randomized block design whit four replicates. Principal component analysis and clustering based on distance between means of 8 morphological traits were used to detect relationship between accessions. Thirty five RAPD primers produced 142 polymorphic bonds, average 4.05 for each primer. Clustering analysis technique based on RAPD using Unweighted Pair Group Method shown that a closely association exist among morphological and RAPD dendrograms while didn`t exist any accordance between ISJ-GS with RAPD and morphological variation. In RAPD–based clustering, all population that was belonged to near area formed closely groups. The ISJ system marker produced 95 DNA fragment with 2.55 polymorphic bands for each semi-random primers. The dendrogram based on ISJ marker did not have accordance with geographical, morphological and RAPD variation. The result of this research verified possibility of use of RAPD and ISJ markers for estimation of genetic diversity, management of genetic resources and determination of repetitive accessions in Plantago ovata.
  S. Bahrami , M. Solouki , B. Siasar and A. Ghanbari
  In order to determine the level of TaGSK1 gene expression in 9 selected wheat genotypes an experiment was carried out in Agricultural Biotechnology Institute, University of Zabol. Seeds of wheat cultured on solid MS medium plate under positive and control condition and RNA was extracted for each genotype and treatment. Relative gene expression using cDNA as template was done in Real Time PCR. The results of real time PCR showed that the Bam genotype has maximum level expression of TaGSK1 gene between 9 genotypes. Minimum expression was found in ER-Salt-85-17 line originated from Tehran Province. TaGSK1 gene expression between genotypes is varied from 39% in ER-Salt-85-17 until 71% in Bam genotypes.
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