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Articles by M. Siddique
Total Records ( 3 ) for M. Siddique
  M. Rahim , M. Siddique , S.T.A.K. Sindhu and Mirza Ali Khan
  Eighty, day-old broiler chicks were primed with live ND and IBD vaccines at 7 and 9 days of age and boosted at the age of 21 and 28 days, respectively. At 3 weeks of age, chicks were divided into 5 equal groups A, B, C, D and E. Groups A, B and C were fed on aflatoxin contaminated feed and 1, 2 and 3 g of Hydrated Sodium Calcium Alumino Silicate (HSCAS)/kg of feed respectively. Group D was raised on contaminated feed without binder (positive control) and group E on normal feed without binder (negative control) for a period of four weeks. Maximum body weight gain was observed in group E and minimum in group D. There was a dose related increase in the weight gains of groups A, B and C. The minimum HI titres against NDV were found in group D and maximum in group E. The gradually increasing dosage of the binder in the feed of groups A, B and C manifested a dose related increase in HI antibody titres. Maximum IHA titres against IBD were found in group E and minimum in group D. Groups A, B and C also manifested a dose related increase in IHA titres against IBD. The mortality recorded in groups A, B, C, D and E was 31.25, 18.75, 18.75, 43.75 and 0.0 percent, respectively. Mycotoxin-binder at the rate of 3 g/kg of feed gave better results in terms of body weight gain, humoral immune response and better protection against aflatoxicosis compared to the dose rates of 1 and 2 g/kg of feed.
  Sajjad-ur- Rahman , M. Siddique , Tahir Hussain , Sarfraz Hussain and M. Ansar
  Five different effective microorganisms (EM) were studied for their antimicrobial activity against various pathogenic bacteria in vitro. Type EM3, EM4 and EM5 showed good antibacterial activity against animal pathogens including Escherichia coli, Salmonella spp., Pasteurella spp. and Staphylococcus spp. There was an inverse correlation between the antibacterial and the higher dilution of the culture. All biologically active EM5, may be used efficiently at the highest dilution of 104, while the EM2 and EM3 proved ineffective against the pathogenic organisms under study.
  T. Mahboob , M. Arshad , H. Afzal and M. Siddique
  Mukteswar strain of Newcastle disease (ND) virus was propagated in 9-day-old embryonated eggs via allantoic route. The harvested allantoic fluid with haemagglutination (HA) titre of 1024 (EID50 109.3) was inactivated using 0.1 percent formalin solution. Two experimental vaccines were prepared in light mineral oil with aqueous to oil ratio of 1:2 ( MK-VAC-I) and 1:4(MK-VAC-II).The hydrophile lipophile balance (HLB) of the vaccine was fixed at 7,0 using oil phase (span 80) and aqueous phase (tween 80) surfactants. One hundred and twenty, four week old layer chicken were divided into four equal groups (A to D). Groups A and B were injected with two experimental vaccines and C with commercial oil emulsion vaccine keeping D group as non-vaccinated control. Highest four week cumulative haemagglutination inhibition titres were shown by commercial vaccine (226) followed MK-VAC-I (217) MK-VAC-II (171) and lowest by control group. Both the vaccine showed 80 percent protection against virulent virus challenge comparable to the commercial one.
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