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Articles by M. Shariff
Total Records ( 3 ) for M. Shariff
  Z.T. Harith , F.M. Yusoff , M. Shariff and A.B. Ariff
  The aim of this study was to optimize and to propose the suitable separation method and storage conditions for specific species of microalgae. The performance of different separation methods for the recovery of cell biomass of marine microalgae, Chaetoceros calcitrans, from the culture broth was evaluated. The microalgae were cultivated using 10 L photobioreactor. The microalgae cell cultures were concentrated either by centrifugation, tangential flow filtration or flocculation and then stored at different temperatures (-20, 4 and 27°C) to investigate the optimum storage conditions for C. calcitrans prior to different downstream processing methods. High concentration of cell in slurry (4.88x107 cells mL-1) was obtained using centrifugation as compared to tangential flow filtration (4.14x107 cells mL-1), flocculation with chitosan (1.56x107 cells mL-1) and flocculation with Magnafloc®LT 25 (8.24x106 cells mL-1). Storage of C. calcitrans biomass at chilled temperature (4°C) directly after the harvesting using these four different separation methods resulted in extended shelf life (> 4 weeks). Frozen biomass (-20°C) fails to preserve the quality of C. calcitrans after they were revived in fresh medium. C. calcitrans flocculated with 0.5 mg L-1 Magnafloc®LT 25 was able to maintain the quality of the cells after storage at 27°C for more than 2 weeks. However, flocculation of cells with 20 mg L-1 chitosan, centrifugation at 8000 rpm for 10 min and tangential flow filtration process at transmembrane pressure of 20 psi failed to retain the quality of biomass after storage for 2 weeks at 27°C.
  A.S. Nursuhayati , F.M. Yusoff and M. Shariff
  Phytoplankton forms the essential base of the aquatic food web and plays a major role in aquatic productivity and ecosystem health. In the estuary and coastal waters, one of the major factors that controls phytoplankton community structure is the salinity changes along the river-sea continuum. The study was conducted to determine spatial and temporal distribution of phytoplankton along the salinity gradient in Perak river estuarine system during the northeast monsoon from November 2009 to February 2010. Four stations were established along the salinity gradient, from upstream where the salinity was 0.0 ppt to the marine area with salinity values of more than 25.0 ppt. The phytoplankton comprised of six main families namely, Bacillariophyceae (diatoms), Chlorophyceae (green algae), Cyanobacteria (blue-green algae), Chrysophyceae (golden-brown algae), Euglenophyceae (euglenoids) and Pyrrophyceae (dinoflagellates). A total of 93 species of phytoplankton were recorded from all the stations throughout the season, with green algae and diatoms dominating the upstream and the marine areas, respectively. On the other hand, the estuarine stations were dominated by both green algae and diatoms. The marine station had the highest (p<0.05) species density during the monsoon season compared to the other stations with a mean total density of 190.6±27.4 cells mL-1, whereas, the lowest (p<0.05) density (73.8±11 cells mL-1) was observed in the upstream station. Multidimensional scaling analysis based on the phytoplankton densities revealed two distinct groups. The first group consisted of the phytoplankton from the marine station, whereas, the other group consisted of the phytoplankton from the estuarine and upstream stations. In general phytoplankton distribution in Perak estuary was more similar to the freshwater rather than the marine plankton community due to a large flow of freshwater into the estuary during the northeast monsoon. This study revealed that salinity was an important factor in determining the phytoplankton distribution in this tropical ecotone.
  J.I. Lai , F.M. Yusoff and M. Shariff
  Outdoor mass culture of microalgae in the tropical area is important to minimize its production cost. This study evaluates the growth of Chaetoceros calcitrans in 120 L annular photobioreactors at indoor temperature (Treatment I, 25±2°C) and outdoor tropical ambient temperature, (Treatment II, 30±6°C). Each treatment was done in duplicates. For both treatments, C. calcitrans was first grown in starter columns of 10 L capacity for a period of 7 days at 25±2°C. After 7 days, the 9 L culture was transferred to the annular photobioreactors and subsequently brought to a final volume of 100 L by adding 20 L fresh medium every 5 days. There was no significant difference (p>0.05) in the dry weight of microalgae grown in natural light and those grown indoor. The results suggest that C. calcitrans can be grown in outdoor conditions, hence, saving time and microalgae production cost for the larviculture industry.
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