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Articles by M. Sharif
Total Records ( 6 ) for M. Sharif
  M. Sharif , M.S. Sarir and Nasrullah
  Arbuscular Mycorrhizal (AM) fungi are of considerable interest because of their ability to form symbiotic associations with 85% plants and their potential to use as a biofertilizer. Rhizosphere soil and roots samples of wheat and maize were collected from marginal and fertile soils of Hazara division from soil series extensively use for the production of wheat and maize crops. Physicochemical characteristics of the soil under investigations were determined. AM fungal spores were isolated from soil, identified and their infections rates in the roots were determined. Data indicated that soil pH values of Hazara division ranged from 6.38 to 7.66, soil organic matter content ranged from 1.20 to 1.97% and lime from 1.5 to 11.5%. Maximum numbers of AM fungal white spores were found in Battagram and Abbottabad, brown spores in Chamba and black spores in Abbottabad and Chamba soil series in fertile soil whereas in marginal soil, high numbers of white spores were found in Dedal, Battagram and Abbottabad, brown spores in Dedal and Mansehra and black spores in Chamba, Jaba and Haripur soil series of this area. In fertile soil, 8 to 43% AM fungal infection rates were noted in roots of wheat crop where as 44 to 56% infection rates were observed in marginal soil of this area. Results suggest that comparatively higher AM fungal spores and their root colonization in wheat and maize crops were observed in marginal soil than fertile soil with varied spores density and infections intensity from one site to another. More AM fungal spores density caused higher roots infection intensity in wheat and maize crops of the area and higher AM infections rates were observed in soil of around neutral pH values with low organic matter contents.
  M. Tariq , M. Sharif , Z. Shah and R. Khan
  An experiment was designed to study the effect of foliar application of micronutrients on the yield, quality and leaf composition of sweet orange, Blood red variety at Shabazgari, Mardan. The experiment was laid out in a randomized complete block design in 23 factorial arrangement. Zinc, manganese and boron were applied as foliar spray at the rate of 0.4, 0.2 and 0.04 kg ha-1, respectively in the presence of 1.56 kg N ha-1 as urea and 0.4 kg surfactance ha-1 (as wetting agent) in 400 L of water. The maximum fruit yield was obtained, when 0.4 kg Zn ha-1 and 0.2 kg Mn ha-1 was sprayed along with 1.56 kg N ha-1 and 0.4 kg surfactance ha-1 in 400 L of water. The minimum % peel was obtained with B alone and minimum % rag with Zn + Mn, maximum fruit size with Zn + B and maximum fruit volume with Zn + Mn. Similarly, % juice in sweet oranges was increased significantly by B alone, reducing sugar by Mn alone and vitamin C contents by Zn + B through foliar spray, suggested that each micronutrient had different role on the quality of citrus fruit. Foliar spray of Zn, Mn and B along with urea significantly increased the concentration of Zn and Mn in citrus leaves, while the concentration of B was not affected with foliar spray, perhaps due to dilution within the citrus tissues. Therefore, it is suggested that either Zn+Mn or Zn+B may be applied as foliar spray in combination with urea and surfactance for getting the maximum yield and improved quality of citrus fruit under prevailing conditions.
  A. Daryani , M. Sharif , A. Amouei and S. Gholami
  Toxocara canis is one of the most common parasites living in the intestine of domestic and stray dogs. A dog eliminates thousands of eggs into the environment that are potential etiological factor for human toxocariasis. The present study was undertaken to determine the prevalence of T. canis in stray dogs in Mazandaran, Iran. In this cross-sectional study, during the period from April to September 2007, 50 young and adult stray dogs were collected by shooting from urban areas of Sari city, Northern Iran. They were necropsied and the gastrointestinal tract was opened. Recovered parasites were fixed in alcohol and stained in carmine. Faecal specimens were also examined by the formalin ether concentration method. A total of 27 adult and 23 young dogs were examined with 11 adults (40.7%) and 19 youngs (82.6%) being infected with T. canis with an overall prevalence of 60%. There were significant differences in the prevalence of infection between adult and young dogs (p = 0.003). There were no significant differences in the prevalence of infection between male and female dogs (p>0.05). Considering the high prevalence of this zoonotic parasite and its hygienic significance in causing human toxocariasis, particularly in children, plus the lack of control of stray dog populations, there is a need to improve personal and food hygiene as well controlling stray dogs in these urban areas.
  A. Daryani , M. Sharif , M. Meigouni , F. Baba Mahmoudi , A. Rafiei , Sh. Gholami , A. Khalilian , Sh. Gohardehi and A.M. Mirabi
  In this study 142 stool samples (64 HIV+/AIDS patients and 78 non-HIV infected individuals) collected from Mazandaran province and screened for intestinal parasites, using direct wet mont, formalin-ether sedimentation concentration, modified Ziehl Neelsen and modified trichrome techniques. Each person in this study was examined for CD4+ counts. Intestinal parasites were found in 11/64 (17.2%) of patients in HIV+/AIDS group and in 14/78 (17.9%) of controls. Prevalence of parasites detected in HIV+/AIDS individuals was as follow: Cryptosporidium sp. 9.4%, Giardia lamblia 3.1%, Entamoeba coli 1.6%, E. histolytica 1.6% and Chilomastix mesnili 1.6%. Prevalence of parasites in controls was as follow: Trichostrongylus sp. 6.4%, G. lamblia 3.8%, Cryptosporidium sp. 2.5% , E. coli 2.5% , E. histolytica 1.2% , Hookworms 1.2%. The mean of CD4+ counts in HIV-positive group (430 cells μL-1) was remarkedly less than controls (871 cells μL-1) (p = 0.00l). As patients usually belong to poor socio-economic backgrounds and they can hardly afford treatment, therefore, it is suggested screening and free treatment of intestinal parasites in these individuals should be taken by health centers to prevent the occurrence of these diseases in HIV+/AIDS patients, as often the disease may take a fulminant form.
  M. Sharif , A. Daryani , G. Barzegar , M. Nasrolahei and A. Khalilian
  This cross-sectional study was carried out on 1210 randomly selected schoolchildren, attending sixteen primary and secondary schools, during the period between November 2005 and June 2006. Parents who accepted to include their children were requested to be present at sampling time and to fill in a simple questionnaire with personal and epidemiological data. Three milliliter of venous blood were taken by vein puncture under sterile conditions from each subject for detection and titration of antibodies to T. canis and eosinophil counts. Total IgG anti-Toxocara antibodies was evaluated by T. canis IgG ELISA kit. According to the manufacture recommendations, an index positivity >11 U was considered positive. Of the 1210 serum specimens tested, an overall seroprevalence for Toxocara antibodies of 25% was obtained. There was no association between positive seroprevalence and age (p = 0.34). Boys and girls differed significantly with regard to Toxocra titre (p = 0.003). Eosinophilia in peripheral blood (≥5%) was detected in 24.5% (297/1210) of the population studied, 97/297 (32.8%) of whom were seropositive for toxocariasis. The findings of this study confirmed that infection with Toxocara is quite high and widespread in children in Northern Iran. Therefore, health promotion efforts must be directed at increasing the awareness of the population about the potential zoonotic hazards associated with the disease and how to minimize them.
  M. Sharif , A. Daryani and S.A. Karimi
  The aim of this study was to elucidate the species diversity of larva trematodes in the Mazandaran Province, Northern Iran. In this cross-sectional study, a total of 3,266 lymnaeid snails from 3 species were collected from different parts of streams, swamps, rice fields and rivers in the central areas of Mazandaran Province (Sari, Neka, Qaemshahr and Savad-Koh cities), during April to September, 2008. The samples were tested by crushing and emerging methods. From the total of examined snails, 119 (3.6%) were found to be infected with the lymnaeid snails. Lymnaea gedrosiana were found to be infected with the Furcocercariae of Diplostomidae, Clinostomidae, Echinostomatidae and also cercariae of the Plagiorchiidae. The latest infection was found to be in L. palustris. The Mazandaran Province with its temperate climate is a suitable place for living of snails, particularly lymnaeidae, that could have a significant role as an intermediate host of diseases.
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