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Articles by M. Samadi
Total Records ( 5 ) for M. Samadi
  S. Jafari , S. Esfahani , M.R. Fazeli , H. Jamalifar , M. Samadi , N. Samadi , A. Najarian Toosi , M.R. Shams Ardekani and M. Khanavi
  The volatile oil from Citrus aurantifolia (Christim) Swingle (lime) fruit peel is abundantly used as flavoring agent in food industries. In this study chemical composition and antimicrobial activity of essential oil Citrus aurantifolia against food-borne pathogens was determined to investigate its potential in reducing microbial population of cream-filled baked goods. Fifty componenets were identified in Citrus aurantifolia essential oil by GC-MS analysis and limonene, α-terpineol and γ-terpinen were the most abundant constituents. The results of bioburden determination showed that cream-filled cakes and pastries were mainly contaminated with Staphylococcus epidermidis and Bacillus subtilis. Lime essential oil showed potent antibacterial activity against spoilage bacteria. MICs (Minimum Inhibitory Concentration) of lime essential oil against S. epidermidis and B. subtilis were determined 4 and 8 μL disc-1, respectively. By using 16 and 32 μL mL-1 of essential oil, more than 99.9% reduction in S. epidermidis and B. subtilis counts were observed, respectively. The use of Citrus aurantifolia essential oil in concentrations higher than MIC value can improve shelf life of cream-filled cakes and pastries. According to our results, lime oil can increase the time needed for the spoilage bacteria to reach concentrations able to produce a perceivable spoilage and it may consequently reduce the risk of diseases associated with consumption of contaminated products.
  S. Shiva , M. Samadi , B. Rastghar , A. Jafari Javid and S.H. Shiva
  This study was designed to evaluate the effect of cardiopulmonary bypass duration on thyroid function in children undergoing open heart surgery. One hundred and five children with congenital heart disease in-need of open heart surgery with cardiopulmonary bypass were enrolled during a 12-month period. Patients categorized into two groups: Short-time cardiopulmonary bypass (<90 min) and long-time cardiopulmonary bypass (≥90 min). Serum levels of Triiodothyronine (T3), Thyroxine (T4) and Thyroid Stimulating Hormone (TSH) were measured 2 h before surgery and 2 and 5 days after operation. If needed another laboratory investigation was carried out on week 2 after operation. There were 46 (28 males, 18 females with the mean age of 5.63±3.64 years) and 59 (30 males, 29 females with the mean age of 4.59±3.18 years) children in short-time and long-time cardiopulmonary bypass groups, respectively (p>0.05). The mean serum level of T3, T4 and TSH was not significantly different between the two groups. In each groups, serum TSH and thyroid hormones were decreased until 2 h and 2 days after post-operation, respectively, with gradual increase afterward. Decline of serum thyroid hormones was more longstanding in the long-time cardiopulmonary bypass group. Two weeks after operation, repeating the same laboratory tests in patients with defective results yielded normal consequences. According to our results, there is a transient laboratory abnormality in thyroid function tests in children undergoing cardiopulmonary bypass. This abnormality persists longer in patients with long-time cardiopulmonary bypass.
  K. Sakha , M. Samadi and A. Rezamand
  The goal of this study was the evaluation of specific markers of myocardial injury that includes CK-MB and troponin I in major thalassemic patients. Regular blood transfusion is the main treatment in major thalassemia. One of the most important complications of regular blood transfusion is iron overload that eventually involves many organs like heart and cause myocardial injury. Sixty patients with transfusion-dependent major thalassemia, at the age range of 8 to 15 years in Tabriz Pediatric Medical Center were chosen. Measurement of Hb, Hct and serum ferritin were performed in hospital laboratory, but total serum Creatine Kinase (CK) by photometric and isoenzyme of CK-MB by immunologic DGKC and cardiac troponin I (cTnI) were tested by ELISA methods in Shaheed Madani heart center laboratory before blood transfusion. For all patients echocardiography and ECG assessment of cardiac function were done by a pediatric cardiologist and results were statistically analyzed. Forty nine patients (group A) had normal left ventricular ejection fraction (LVEF = 50-70%) and 11 patients (group B) had reduced LVEF (20-45%). There was no statistical difference between two groups in average volume of blood transfusion (p = 0.074). Although total CK and CK-MB isoenzyme were higher in group B but there was no statistically meaningful difference between two groups (p = 0.123, p = 0.111). Troponin I also was higher in group B but statistically analysis showed no correlation between cardiac function and troponin I level in these groups (p = 0.827). This study showed that cardiac markers are not helpful for recognition of cardiac involvement in major thalassemia.
  M. Nobakht , S. Majidzadeh , M. Fattahi , M. Samadi and P. Tabatabaeei
  Abstract: The expression of neuronal nitric oxide synthase during the development of rat optic vesicle from embryonic day E14 to E18 was analyzed by histochemical procedures. The samples were frozen and cut on a cryostat and then studied by using the light microscope. Expression of nNOS was first seen on E14 in cells of Cajal-Retzius located in the marginal zone of optic vesicle. NADPH-d persisted in this layer throughout the embryonic period and began to decrease on E20. At E16, the optic vesicle has four NADPH-d positive layers. At E18, NADPH-d reactivity observed at low magnification showed five clearly defined layers. In the late stages, the most notable feature was a decrease in histochemical reaction of the marginal zone and at these stages, the layer IV showed less staining than the rest of the cortical plate. The observations suggest that nitric oxide is synthesized during embryonic life processes and this is related to maturational processes.
  M. Samadi , R.J. Rashid , S. Ghaffari and M. Shoaran
  The aim of this study is to evaluate the growth failure in children with Congenital Heart Diseases (CHD) associated with the Pulmonary Hypertension (PH) and cyanosis. Growth parameters including weight, height and head circumference of 120 cases with congenital heart defects aged 6 months to 14 years were compared with standard growth curves (50th percentile) between November 2007 and November, 2008. Of all, sixty five (54.1%) were male and 55 (45.8%) were female. The patients were classified into four groups based on the presence or absence of PH and cyanosis. The gap between chronological age and bone age (BA) for all subjects was determined. Growth disturbance in weight, height and head circumference was detected in 80 (66.7%), 79 (65.8%) and 41(34.2%) of the patients, respectively. Bone age delay was seen in fifty five percent of the cases. Generally, delay in all parameters was more seen in acyanotic patients with pulmonary hypertension. In subjects with cyanosis whether in addition to PH or not, bone age was significantly retarded. Etiology of growth failure in children with CHD is multifactorial. Further studies are required to assess the role of different factors in this field.
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