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Articles by M. Rozihan
Total Records ( 5 ) for M. Rozihan
  M. Rozihan , E. Ismail , B. Akbar John , K.C.A. Jalal and A. Mohd-Adnan
  The genetic structure of horseshoe crab (Tachypleus gigas) populations were examined using 18 microsatellite markers. Samples were collected from 2 stations of east coast of Peninsular Malaysia and one station from East Malaysia. All the observed allelic frequency showed no significant variation between sampling stations (p>0.05). Mean allelic richness (Ar) was greater in Sarawak population (4.83) followed by Pahang (4.24) and Terengganu (3.57) samples with the overall mean allelic richness of 4.21. Mean inbreeding coefficient (FIS) value was higher in Sarawak population (0.37) followed by Terengganu (0.31) and Pahang population (0.17). The genetic differentiation (FST value) and genetic distance between geographically closer populations was smaller compared to geographically isolated populations. Due to the dwindling population size of horseshoe crabs in Malaysian coast line, present data provides new information in assisting proper management and conservation of this living fossil.
  M. Rozihan , B.A. John , C.R. Saad and K.C.A. Jalal
  The effect of partial and complete substitution of fishmeal (FM) for Soy Bean Meal (SBM) on the growth and survival of Macrobrachium rosenbergii were studied. Post larvae (PL 28) with an average individual length (16.20±0.20 mm) and weight (0.032±0.002 g) were stocked in aquarium filled with 5.0 L of water for a period of eight weeks. Six types of diets with different percentage of FM:SBM ratios were formulated and fed to the test animals at the ratio of 0:100, 20:80, 40:60, 60:40, 80:20, 100:0 (diet 1-6, respectively). Significant differences in terms of body weight and orbital length of animal were observed between diets 2, 3, 4, 5 and diets 1 and 6. Larvae fed with diet 3 produced the highest mean weight gain 0.711 g. There was no significant difference in weight gain observed in experiment provided with diet 2, 4 and 5 (~0.4-0.5 g). Larvae fed with diet 1 and 6 showed less weight gain (~0.2 g) with highest percentage of survival (70%) in diet 6 experimental tank. Food Conversion Ratio (FCR), Protein Efficiency Ratio (PER) and Specific Growth Rate (SGR) were also calculated for each experimental diets besides analyzing their proximate composition. It can be concluded that soy bean (diet 3) could be used for the better growth of M. rosenbergii post larvae. It was also understood that complete removal or absolute utilization of fish meal (100%) in diet would directly affect the weight gain in fresh water prawn (p<0.05).
  M. Rozihan and E. Ismail
  A detailed investigation was carried out to determine the genetic structure and haplotype diversity of Malaysian horseshoe crab (Tachypleus gigas [Muller, 1785]) distributed along the west coast of Peninsular Malaysia. Mitochondrial DNA (AT rich region = 369 bp) analysis showed that T. gigas had 13 haplotypes along the Malaysian west coast of which 4 were unique to Selangor samples while 3 were unique to Johor sample and 1 each were unique to other two stations respectively. Highest haplotype diversity (h) was observed among the Selangor samples (0.873±0.071) followed by Langkawi, Johor and Kedah samples with 0.833±0.222, 0.752±0.066 and 0.733±0.155 values, respectively. Over all haplotype diversity of T. gigas in west coast of Malaysia was observed to be 0.797±0.129. Pair wise haplotype frequency (FST) value were statistically significant (p<0.05) for all the groups except for Langkawi/Kedah samples indicating higher gene flow (Lower haplotype diversity) among these two populations. Average nucleotide diversity (π) was higher in Selangor samples (0.0083±0.001) followed by Johor (0.0063±0.0011) and it was almost similar in Langkawi (0.0045±0.0012) and Kedah (0.0040±0.0008) samples which indicated higher polymorphic sites in Selangor and Johor samples while it was lower in Langkawi and Kedah samples. In addition phylogenetic analysis clearly clustered T. gigas samples from T. tridentatus samples indicating good phylogenetic signals in mtDNA AT rich region. Overall, findings from this study have important implications for proper management and conservation of this living fossil along the west coast of Peninsular Malaysia.
  K.C.A. Jalal , A.A. Shamsuddin , M.F. Rahman , N.Z. Nurzatul and M. Rozihan
  A study was conducted to determine the total content of carotenoids, in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant aeration (4.5 L min-1) at 8 pH. Salinity of the water was maintained between 20-24 g L-1 and temperature range was 20-24°C. The culture of Isochrysis sp. showed a short lag phase lasting about 24 h followed by exponential phase starting from day 2 until day 10. In day 4, specific growth rate (μ) for Isochrysis sp. was 1.6059 day-1, the highest rate compared to other day. Stress parameter (NO3¯ starvation) was introduced into the mass culture on day 4 by eliminating NO3¯ in f/2 media. There was considerable declining (p<0.05) in cell count was observed in the absence of nitrogen proving the dependency of carotenoid production over the nitrogen limitation which probably due to the main role played by the nitrogen in various metabolic activities of the cell. The highest total carotenoid per cell was recorded at day 10 with 0.001932347 mg mL-1, whereas the lowest total carotenoid content per cell was observed at day 4 with 0.000100649 mg mL-1. The finding reveals that it is best to harvest the carotenoid on day 10, when the maximum carotenoid can be obtained.
  C. Prasanna Kumar , B. Akbar John , S. Ajmal Khan , P.S. Lyla , S. Murugan , M. Rozihan and K.C.A. Jalal
  An effort was made to assess the utility of 650 bp partial Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members of taxonomically ambiguous marine fin fishes (Family: Mugilidae). To address the issue we used all the 95 barcode sequences of Mugilidae family available at NCBI (National Centre for Biotechnological Information) along with the barcode data generated from Mugilidae fishes of Parangipettai coastal waters. The average GC content of Mugilidae was found to be 46.46%. Crenimugil crenilabis showed less GC content (44.55%) whereas Liza macrolepis showed high GC content (48.53%) among the mullet species studied. The phylogenetic and genetic distance data showed that Mugil platanus and M. liza represent the continuum of same species. Among the members of family Mugilidae, the genus Mugil might possibly contains more haplotype diversity as revealed by intra-species genetic distance data. Species within genera of Mugilidae family invariably clustered in single clade with high bootstrap value. We conclude that partial COI sequencing (barcoding) in identifying the members of the family and that way has resolved the taxonomic ambiguity among the members of the family Mugilidae.
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