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Articles by M. Nakamura
Total Records ( 3 ) for M. Nakamura
  X. Zhang , X. Wang , B. Wang , T. Sugi and M. Nakamura
  The onset detection of muscle activation is an essential issue in electromyogram (EMG) control. In this paper, a novel approach based on EMG power with automatic adaptive threshold is proposed to address this issue. The purpose is to develop an effective EMG-controlled meal assistance robot. Taking into account the individual difference such as contraction power and resting power, the threshold of onset detection is set with respect to the latest EMG signal. The results show the method is able to adjust automatically to avoid false alarms, and works well when the contraction power varies. Implementation of this EMG-controlled meal assistance robot may provide limb-deficient patients with an effective and comfortable human-machine assistance interface.
  M. Nakamura , K. Sugimoto , H. Asano , H. Murakawa , N. Takenaka and K. Mochiki
 

Neutron radiography is suitable for the visualization of liquid behavior in a metallic machine. Observation of oil behavior in a small 4-cycle engine on operating was carried out by using the neutron radiography facility at JRR-3 in JAEA. The engine was not fired but operated by an electrical motor. Movies were taken by a neutron image intensifier with a color CCD camera of 8-bit resolution, 30 frames/s and 640×480 pixels developed by Toshiba Corp. The engine was placed on a turn table and was rotated, so the movie could be taken from any angle. Numbers of revolution of the engine were changed from 260 to 1200 rpm. Visualized images of the mechanism and the oil behavior in the engine were obtained.

  Y Fujii and M. Nakamura
 

DNA mismatch repair is an important mechanism in the prevention of mutations. We reported the existence in the HL60 cell line of a novel G/T-mismatch DNA-binding protein (nGTBP) requiring strict DNA sequences. In this report, we identify the FOXK2 transcription factor as the nGTBP. FOXK2 fragments were obtained as the only clones with specifically binding activity to the G/T-mismatch DNA by screening of a human brain expression library. The recombinant forkhead domain of FOXK2 specifically recognized the G/T-mismatch DNA. The forkhead domain also recognized hypoxanthine/T and G/uracil, derived from the deamination of the exocyclic amino groups of A/T and G/C, respectively. An electrophoretic mobility shift assay (EMSA) analysis using HL60 cell nuclear extract and antibody raised against FOXK2 resulted in the exclusive binding of FOXK2 to G/T-mismatch DNA. Furthermore, FOXK2 bound to G/T-mismatch DNA with higher affinity than ‘match’ FOXK2 consensus DNA. We therefore propose that FOXK2 is a G/T-mismatch DNA-binding protein and a deaminated DNA-binding protein.

 
 
 
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