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Articles by M. Musa
Total Records ( 3 ) for M. Musa
  V.K. Gopinath , M. Musa , A R. Samsudin , K. A.M. AI-Salihi and W. Sosroseno
  The aim of the present study was to assess phagocytic activities of murine macrophages (RAW264.7 cell line) to hydroxyapatite (HA) particles. Transmission electron microscope (TEM) was used to assess localization of HA particles in the cells at 7, 15, 30 and 60 min. The HA particles and latex beads, as a control, were also incubated with the cells pretreated with various concentration of cytochalasin B or colchicine. Phagocytic index (PI) was used to determine the phagocytic activity at one hr of incubation. TEM analysis showed that HA particles were in direct contact with the cells at 7 min with a few particles located within cell vacuoles. The cells increasingly phagocytosed the particles up to 60 min. Cytochalasin B or colchicine significantly inhibited phagocytic activity of the cells to both HA particles and latex beads in a dose-dependent fashion. Therefore, the results of the present study suggest that HA particles may induce phagocytic activity of RAW264.7cells in an actin and microtubule polymerization-dependent mechanism.
  N.M. Budari , M.F. Ali , K.H. Ku Hamid , K.A. Khalil , S. Zainal and M. Musa
  This study was conducted to discover ultrasonic treatment as an innovative technology specifically for Escherichia coli wild type cell disruption for the disinfection process. Therefore, this study aims to evaluate the influence of process conditions through the performances of Flow Cell Ultrasound (FCUS) as the bacterial disruption. In order to determine the influence of the process condition through flow cell ultrasound, the research approach used in this study was measured by the disruption of Escherichia coli cells with inoculum (104 CFU/mL), sonication media Phosphate Buffer Saline (PBS) and peptone water, 35% amplitude and flow rate (70 mL/min). There was statistically a significance difference (p<0.05) between peptone water and PBS where PBS as the sonication media showed 6.93% higher cells disruption as compared to the peptone water. The percentage removal at 120 min treatment for cells disruption exhibited 98.55±2.51% these interpreted that the disruption of Escherichia coli wild type cells comply with first-order kinetics model and k = 0.0353 min-1. Furthermore, ANOVA elucidated that the temperature increases by time and p<0.05 and resulted in an average final temperature of 28.6°C which was within the non-lethal temperatures for Escherichia coli. Thus, the use of flow cell ultrasound was identified to be beneficial uses for water disinfection due to green process occurred during their removal performances.
  L.U. Onyia , M.Y. Diyaware , K.G. Michael , M. Musa and I.J. Ochokwu
  The study on haematological indices of Clarias gariepinus and Clarias anguillaris was carried out to compare and contrast their blood group, genotype and haematological profile. Little is known about the blood group and genotype of the species. The knowledge of the blood group and genotype will be useful in the genetic study of the species. Test tube techniques based on agglutination tests was used for blood grouping, while the genotype was determined by hemoglobin electrophoresis. The haematological parameters, blood group and genotype of Clarias gariepinus (n = 12) and Clarias anguillaris (n = 12) males and females, were studied. The mean value of WBC count (μL) in C. gariepinus males was 1621.67±10.83 μL and females 9033.33±13.63 μL, respectively. The mean RBC counts for males were 3.0833±1.23 μL and females 3.85±0.46 μL and females 3.8500±0.46 mm3, respectively. The mean MCHC for males and females were 33.15±0.1 g dL–1 and 33.08±0.1 g dL–1, respectively. Mean PCV for males was 51.62±6.79% and females 65.02±18.63%, respectively. Mean Hb. for males was 18.62±1.87 (g dL–1), while the females had mean 22.12±5.823 (g dL–1). The mean MCV for males and females were 172.17±43.03 and 169.300±31.64554 pg. The genotype of all the males and females were the same (AA). The blood group recorded 83% with O+, while 18% had AB+, respectively in the males and females. The WBC counts of males and females C. anguillaris were 7733.33±648.90 and 8033.33±16.47 μL, respectively. The mean RBC counts for males and females were 2.60±0.45 μL and 2.60±0.44 μL, respectively. The MCHC of both males and females were the same 33±0.00 g dL–1. The mean PCV of males and females were 87.2±1.97 and 66.73±30.93%, respectively. The mean Hb for males and females were 29.77±1.20 and 22.47±10.13 g dL–1, respectively. The mean MCV for males and females were 356.97±6.16 and 274±92.60 pg, respectively. The blood group, genotype and agglutination tests results were 83% Rh- O+, 18% Rh-AB+ and 100% AA, respectively and were similar to the pattern in humans.
 
 
 
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