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Articles by M. Motovali-Bashi
Total Records ( 2 ) for M. Motovali-Bashi
  H. Korbekandi , D. Abedi , M. Pourhossein , M. Motovali-Bashi , M. Hejazi , M. Narimousaei and M. Kabiri
  We aimed to optimize esterase production of Candida rugosa lipase (CRL). Active culture of C. rugosa (DSM 2031) was revived and the culture medium containing the most frequently used ingredients was optimized using a fraction of factorial design method, Taguchi. Temperature and pH of the culture was also optimized using one factor at a time method. The optimum combination of the major medium ingredients, in order of their magnitude, was (g L-1): Corn Steep Liquor (CSL) powder, (40), triolein (glyceril trioleate) (10), glucose (0) and oleic acid (2). The optimum temperature and pH were 30°C and 7, appropriately. Using this combination and conditions, esterase activity of the enzyme preparation was increased up to 9 U mL-1, which was equivalent to 20611 U mL-1 of Sigma® lipase lipolytic activity.
  M. Motovali-Bashi , Z. Hojati and Richard M. Walmsley
  Homologous recombination repair starts with Double-strand Breaks (DSBs) followed by crossing-over and recombination. The expected frequency of meiotic chromosomal exchange in the region of chromosome XII encoding ribosomal DNA in Saccharomyces cerevisiae is 3.5 to 5 events per cell per meiosis. However interchromosomal meiotic recombination in the rDNA gene is very rare, suggesting repression of DSB and crossing-over. On the other hand, mitotic events such as intrachromosomal recombination producing 3 μm rDNA circles (which accumulate with cellular age) and unequal sister chromatid exchanges appear to be quite common. This study looked at the rDNA breakage in the strain ORD 1181, a rad50S mutant with SK1 background, which does a relatively fast and near synchronous meiosis. The fine analysis of the rDNA array was performed using restriction endonuclease enzymes that do not cleave within the rDNA array. The results suggest that there are at least two hot regions for chromosome breakage within the rDNA array. According to our previous studies we suggest that the DSB hot regions are in one homologue. However, there is possibility that other homologue is involving in DSB too.
 
 
 
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