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Articles by M. Mohebali
Total Records ( 3 ) for M. Mohebali
  M. Nateghpour , M. Sharbatkhori , G.H. Edrissian , E. Souri , M. Mohebali , K. Akbarzadeh , A. Motevalli Haghi , M. Satvat and A. Rahimi
  In this study antiplasmodial activity of Peganum harmala was assessed against isolates of Chloroquine -sensitive and Chloroquine-resistant plasmodium falciparum, collected from southeast of Iran, in comparison with Chloroquine. Peganum harmala extract in four serial dilutions including 12.5, 25, 50 and 100 μg mL-1 and chloroquine with 8 picomol/well in the test well were evaluated using in vitro method on 23 blood samples from patients infected with Plasmodium falciparum. All of four serial dilutions of Peganum harmala extract could inhibit parasite growth (p<0.05). In comparison with Chloroquine at doses of 12.5 and 25 μg mL-1, it was less effective than Chloroquine, while 50 and 100 μg mL-1 had almost similar results to Chloroquine. P. harmala in doses of 50 and 100 μg mL-1 could decrease Significantly schizont formation of those two strains of P. falciparum parasites which were resistant to chloroquine.
  J. Saki , L. Akhlaghi , S. Maraghi , A.R. Meamar , M. Mohebali , H. Oormazdi , E. Razmjou , S. Khademvatan and F. Rahim
  This study aims to evaluate the modified Novy-MacNeal-Nicolle (NNN) medium by adding mycosel agar to inhibit the growth of fungi and comparing it to traditional culture for the isolation of Leishmania parasites from cutaneous lesions of patients presenting to a specialized leishmaniasis clinics in Iran. A total 128 (70 female and 58 male) consecutive patients aged 3 months to 75 years (mean age of 11.25 years), presenting to Lesion biopsies were cultured in duplicate and parallel in traditional culture tubes containing NNN medium and modified NNN medium. The contamination rate in two groups of media was assessed. After sufficient incubation we have investigated that the media tubes for the presence of fungi. Through 90 samples, in 46 NNN media and 58 mycoNNN promastigotes were growth and culture positive. The difference between NNN medium and Mycosel NNN was quite significant (p<0.05). The overall sensitivity of Modified NNN medium were 75%, while traditional culture had sensitivity of 64.8%. We have demonstrated that modified NNN medium is a more sensitive and time-efficient means of isolating Leishmania parasites from cutaneous lesions than traditional culture. Our study showed that contamination of NNN medium as base culture medium for isolation of Leishmania agents could be diminished significantly by addition of mycosel agar to NNN medium.
  P. Ghaffarinejad , M. Farahmand , H. Nahrevanian , M. Mohebali , F. Zaboli , Z. Zarei , B. Akhoundi , M. Barati and F.S. Ghasemi
  Leishmaniasis is a parasitic disease, caused by several Leishmania species. Clinical symptoms range from Cutaneous Lesions (CL) to severe Visceral Leishmaniasis (VL). The main cause of VL in Iran is Leishmania infantum and domestic dogs are the major reservoir host. Different methods are used for diagnosis of VL including the parasitology staining from tissue aspiration and determination of antibodies against VL. The aim of this study was to compare direct agglutination (DAT) and rKE16 dipstick rapid tests for VL in domestic dogs from Meshkin Shahr district, Ardabil Province, Northwestern Iran. Blood samples of 200 dogs from five different regions were collected and their sera tested with DAT and rKE16 methods. Collected data were compared for VL detection, antibody titers and their association with age, gender and location. The highest rate of infection was detected by DAT (24.5%) in compare to rKE16 (11.5%). Majority of dogs suffering from VL were 2-7 years old and more male infection (82%) was observed with the highest rate in Parikhan village. These findings showed that DAT has a higher sensitivity than rKE16 dipstick for dogs VL detection. More infection was observed among dogs from Parikhan village of Meshkin Shahr district, Ardabil province, Northwestern Iran.
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