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Articles by M. Moeini
Total Records ( 2 ) for M. Moeini
  M. Ziyaeyan , A. Alborzi , M. Jamalidoust , A. Japoni , P. Badiee and M. Moeini
  Herpes simplex keratitis is one of the important causes of blindness and its early diagnosis is essential to the treatment of the respective patients accordingly. The aim of this study was to diagnose and analyze quantitatively the herpes simplex DNA in patients with suspected Herpes keratitis using TaqMan real-time PCR method. Corneal swabs from HSV keratitis suspected patients were collected from September 2005 to December 2009. Upon DNA extraction, the samples were analyzed by quantitative TaqMan real-time PCR assay. In this method, a set of primers amplified a common sequence of HSV-1 and HSV-2 glycoprotein B gene. The copy number of unknown samples were expressed via standard curve drawn with known amount of amplified cloned plasmid containing target sequences. Of the 296 samples, 178 (60.1%) belonged to males and 118 (39.9%) to females. The HSV DNA was detected in 111 (37.5%) of the cases by PCR, consisting of 69 males and 42 females. The drawn standard curve was linear in 10 to 106 copies of virus (R2 = 0.982). The ranges of the HSV DNA copy numbers in the collected samples were detected from 2.7x103 to 6.1x105 copies/samples. Present results suggest that Herpes simplex keratitis remains as an epidemiologically important eye disease and improvement in the monitoring and detecting of ocular herpetic disease by quantitative PCR method is informative and helpful to the clinical diagnosis, decision for treatment and follow up.
  M. Moeini , H. Amanlou , A. Aghazadeh , K. Nazer-Adl and M. Aboozar
  The objectives of this study were to determine whether liquid feeding or TMR feeding of glucogenic and mineral supplements can improve metabolic status and plasma metabolits in Holstein fresh cows. Multiparous cows (n = 24), second lactating cows (n = 24) and first lactating cows (n = 24) were used in a completely randomized block desighn, immediately after parturition with 3 weeks experimental period treatments were: Control Treatment (CONT) without any aditive on water or TMR (CONT), Derenching treatment (DRCH) include: 40 L water contained: 2 kg of calcium propionate, propylene glycol, potassium chloride, magnesium sulfate, sodium phosphate, sodium chloride, sodium bicarbonate, choline chloride and methionine, feeding treatment (FEED) contained 2 kg of drenching diet contents that were mixed with TMR, compound diet (DRFD), 20 L water contained 1 kg of drenching treatment plus 1 kg of these materials that mixed to TMR. Treatments had significant effects on Ca, Mg and P concentrations of plasma (p<0.05) and these metabolites had more concentrations in DRCH treatment than others. DRCH treatment had positive effects on negative energy balance and plasma NEFA and β-HBA concentrations. Plasma glucose had increased significantly by treatment. Therefore, addition of these glucogenic precursers and minerals on water of fresh cows resulted in optimize animalmetabolic status in postpartum period.
 
 
 
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