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Articles by M. Minami
Total Records ( 4 ) for M. Minami
  M. Asaduzzaman , M.A. Bari , M. Rahman , M. Minami , K. Matsushima and K. Nemoto
  Agrobacterium mediated transformation has already been reported in rice but under the present investigation efforts has been made to establish the transformation protocol in Bangladeshi rice variety BRRI dhan-30. High percentage of callus induction at 97.3% was obtained when seeds of rice (Oryza sativa L.) cv. BRRI dhan-30 were cultured on modified N6 medium supplemented with 3% sucrose, 2.0 mg L-1 2, 4-D and 0.8% agar under dark condition. Maximum  88.28%  calli  induced  shoots  and  were  obtained from BRRI dhan-30 in the suitable regeneration medium was MS medium supplemented with 3% (w/v) sucrose, 2.0 mg L-1 BAP+0.05 mg L-1 NAA+3% sorbitol and 0.8% agar. Agrobacterium mediated gene transfer protocol for rice variety BRRI dhan-30 were performed using Agrobacterium tumefaciens strain LBA4404, which harbored the plasmid pCL3 containing genes for β-glucuronidase (GUS) and kanamycin resistance. It was found that kanamycin concentration up 50 μg mL-1 were effective for selection of transformants. Putatives transformants as  indicated  by  kanamycin  test  were  subjected  to GUS assay. GUS activities were found in rice calli after co-cultivation.
  H. Yokoyama , M. Matsushima , K. Kawai , K. Hirao , M. Oishi , H. Sugimoto , H. Takeda , M. Minami , M. Kobayashi and H. Sone
  Aims  To investigate whether a reduced incidence of cardiovascular disease in Type 2 diabetes can be achieved in a newly recruited cohort following the recently advanced concept of multifactorial treatment and followed in primary care settings as compared with earlier cohorts.

Methods  A prospective study was performed in primary care settings at multiple clinics nationwide in the Japan Diabetes Clinical Data Management (JDDM) study group. Subjects were 2984 patients with Type 2 diabetes without prevalent cardiovascular disease. The main outcome measure was the first event of non-fatal or fatal coronary heart disease, ischaemic stroke or peripheral artery disease, and the incidence was compared with other representative cohorts.

Results  There were 90 cardiovascular events over 10 827 person-years of follow-up with a dropout rate of 6%. The incidences (per 1000 person-years, 95% confidence interval) of composite, coronary heart disease, ischaemic stroke and peripheral artery disease in the JDDM study were 8.3 (6.6-10.0), 4.4 (3.2-5.6), 3.1 (2.1-4.2), and 0.7 (0.2-1.2), respectively. Each incidence was lowest in the JDDM study compared with other cohorts (P < 0.01 vs. each cohort). In the JDDM study, significant variables predictive of the occurrence of a cardiovascular event were age, duration of diabetes, HbA1c, HDL cholesterol and urinary albumin.

Conclusion  The novel finding of low cardiovascular disease occurrence in this study may be conferred by the feasibility at primary care settings for providing patients with Type 2 diabetes with favourable control of blood glucose, blood pressure and lipids, coupled with unique ethnicity/country factors.

  M. Asaduzzaman , M. Minami , K. Matsushima and K. Nemoto
  An interspecific hybrid of the Fagopyrum species F. tataricum cv. CT-1 (2x and 4x) and F. esculentum cv. Botansoba (2x) and GreatRuby (4x) was obtained through in vitro ovule culture. Seven to ten days old ovules were excised and cultured on 1/2 MS and MS media and rescued ovules were subjected to different combinations and concentrations of BA, NAA, IAA and zeatin added individually and in various combinations along with sucrose. Effects of the hormones were used to evaluate their potentialities for direct regenerations, callus induction and callus regeneration through ovule culture in interspecific cross between tartary and common buckwheat. Interspecific hybridization in Fagopyrum used ovule culture two methods development in this study. The result showed that no single medium was adequate to ensure complete development of the fertilized ovules to plantlets, thus necessitating a sequential three step transfer to different media. RAPD-PCR analysis confirmed hybridity of the regenerated plant.
  M. Asaduzzaman , M. Minami , K. Matsushima and K. Nemoto
  Cultivated tartary buckwheat (Fagopyrum tataricum) was successfully hybridized with cultivated common buckwheat (Fagopyrum esculentum). Both diploid (2n = 16) and tetraploid (2n = 32) and hybrids were produced from interspecific crosses using ovule rescue method. The produced hybrid was confirmed using plant morphological characters, cytological characters and DNA analysis. The morphological characteristics of the hybrids and parent species are described along with estimation of genetic variation of the hybrids. Self-compatibility together with homomorphism has been introduced from F. tataricum to F. esculentum. Inheritance of flower color in the hybrids was found to be intermediate, whereas pin-style flowers and round shape seeds were found to be dominant over homomorphism and non-winged seeds. There were no obvious differences between the two species in efficiency of pollination. The meiotic observations were accorded with high seed fertility of F1 hybrids and produced normal seeds. These observations indicated that the genetic resemblance and chromosome affinity in the hybrids and parental chromosome supported the fertility of the hybrids.
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