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Articles by M. Mahmoudi
Total Records ( 4 ) for M. Mahmoudi
  A.R. Khosravi , M. Riazipour , H. Shokri , M.L. Mousavi and M. Mahmoudi
  The aim of the study was to evaluate the protein similarity degree among 15 C. albicans isolates with different virulence obtained from healthy and infected human and animals. Yeast cells were grown in YPG medium and collected by centrifugation. After cell wall disruption, the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE techniques. Similarity degree was determined using the Dice similarity coefficient (SD). Candida albicans isolates showed high similarity together with average 86.2% (78.1<SD<100). Regarding to the virulence and host aspects, the means SD of high virulent and low virulent isolates as well as human and animal isolates were 85.8 and 85.6% in PAGE, respectively. Considering SDS-PAGE, electrophoregrams of C. albicans cytoplasmic proteins of low and high virulent isolates as well as human and animal isolates showed the similarity about 90%. The mean SD of cell wall was 91.9% between low virulent and high virulent isolates and 92.2% between human and animal isolates. Such results suggest a highly protein similarity degree among the most isolates with low and high virulence as well as from human and animal hosts. The whole-cell protein profile obtained by these techniques could provide additional criteria for the serologic and immunologic studies of C. albicans.
  Y. Yahyapour , R. Hamkar , A. Moradi , M. Mahmoudi , Z. Nourozbabaei , Z. Saadatmand and T. Mokhtari Azad
  Present study introduces results of common influenza virus strains in the north of Iran. Samples collected from 65 patients with acute respiratory illness by throat washing and swabs, randomly from north of Iran (Mazandaran, Golestan and Guilan provinces). The patients suffered from fever (high), cough, sore throat, general malaise, chill and myalgia. Viruses were isolated by cell culture and confirmed with HA (Hemagglutination) test and then typed by and HI (Hemagglutination Inhibition) test. Out of 65 throat samples, 12 influenza viruses were isolated and typed. Isolated viruses belonged to A (H1N1), A (H3N2) and B influenza viruses. This study showed that Influenza viruses displayed identical pattern to other provinces in Iran and to other countries. To fight against epidemics and pandemics, we should collect enough data about status of influenza each year and data of exact vaccine formulation application for use in different areas of the world. Acquired data has shown that the vaccine for above viruses that confirmed by WHO can result in decreased risks of influenza in at risk cases in provinces of north Iran.
  S. Movassaghi , G. Saki , F. Javadnia , M. Panahi , M. Mahmoudi and F. Rhim
  MBCD and Cholesterol-Loaded-Cyclodextrin (CLC) were examined for their abilities to increase the cryosurvival of C57BL/6 mouse sperm, the main strain of genetically engineered mice. The intactness of acrosome and motility of frozen/thawed spermatozoa were used to monitor cryosurvival. In this experimental study, male mice were randomly divided in 6 groups: control 1, experimental 1, experimental 2, control 2, experimental 3 and experimental 4. In experimental groups 1 and 2 spermatozoa were exposed to 0.75 and 1 mM MBCD and in experimental groups 3 and 4 were exposed to two different concentrations of CLC (1 and 2 mg mL-1) over a period of 1 h and were subsequently cryopreserved. Spermatozoa in control 1 group were frozen without any exposure to CLC or MBCD and in control 2 (vehicle), sperms were incubated with 4 mM MBCD. The post-thaw sperms were evaluated for their motility and acrosomal status. The values of the intact acrosome and motility increased significantly with concentration of CLC compared to controls and MBCD experimental groups (p<0.05). These results indicate that cryosurvival of C57BL/6 mouse spermatozoa is enhanced by exposure to MBCD which loaded with cholesterol (CLC) before freezing and MBCD alone can not protect sperm from freeze-thaw damage efficiently compare to CLC.
  M. Mahmoudi , A. Simchi , M. Imani , P. Stroeve and A. Sohrabi
  Magnetite (Fe3O4) nanostructures with different morphologies including uniform nanoparticles, magnetic beads and nanorods were synthesized via a co-precipitation method. The synthesis process was performed at various temperatures in the presence of polyvinyl alcohol (PVA) at different concentrations. It is shown that small amounts of PVA act as a template in hot water (70 °C), leading to the oriented growth of Fe3O4 nanorods, which was confirmed by selected area electron diffraction. Individually coated magnetite nanoparticles and magnetic beads were formed at a relatively lower temperature of 30 °C in the folded polymer molecules due to the thermo-physical properties of PVA. When a moderate temperature (i.e. 50 °C) was used, nanorods and nanobeads co-existed. At higher concentrations of PVA (polymer/iron mass ratio of 5), however, the formation of magnetic beads was favored. The nanorods were shown to be unstable upon exposure to electron beams. Freezing/thawing process was applied post synthesis as temperature programming to fabricate stable nanorods with rigid walls.
 
 
 
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