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Articles by M. Li
Total Records ( 8 ) for M. Li
  X Mu , Y Chen , S Wang , X Huang , H Pan and M. Li
 

Vascular endothelial growth factor-correlated chemokine 1 (VCC-1), a novel chemokine, is hypothesized to be associated with carcinogenesis. VCC-1 is expressed in hepatocellular carcinoma (HCC) cells, but its function remains unknown. To investigate the molecular effects of VCC-1 on HCC cells, the HCC cell line SMMC7721 was stably transfected with the recombinant plasmid pcDNA3.1/VCC-1. Our data demonstrated that overexpression of VCC-1 in SMMC7721 cells significantly enhanced the cellular proliferation, invasive ability, and tumor growth, when compared with both empty vector control cells and parental cells. These results strongly suggest that VCC-1 plays an important role in SMMC7721 invasion and tumor growth, and indicate that VCC-1 may serve as a potential biomarker for anti-HCC therapies.

  S. Guan , H.L. Zhou , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  This study investigated the H-FABP mRNA expression levels in Hainan Black goats. Males from 1-4 months of age beyond adult animals were selected. Three animals of each age were slaughtered to collect samples from heart skeletal muscle (Longissimus dorsi muscle, psoas major muscle, biceps femoris muscle) adipose tissue liver and kidney. The total RNA was obtained to investigate the H-FABP mRNA expression levels by real-time PCR. The results showed that the H-FABP mRNA expression levels in heart and skeletal muscle have the same basic trends at different growth periods. The highest H-FABP mRNA expression in heart and skeletal muscle appeared at 2 months of age and it was extreme higher than those at other ages (p<0.01). The lowest H-FABP mRNA expression level in these tissues were at 1 month old. H-FABP mRNA expression level in adipose tissue was different from those in heart and skeletal muscle. The highest H-FABP mRNA expression in adipose tissue appeared at adult age and its expression was extreme higher than those in younger ages (p<0.01). In addition, the H-FABP mRNA expression pattern in liver was different from the other tissues. The expression in the liver at 1 month of age was significantly higher than those at 2-4 months of age. However, the highest level occurred at adult age. The differences in H-FABP mRNA expression level at 3 and 4 months old were not significant in liver, heart, skeletal muscle and adipose tissue. On the other hand, H-FABP mRNA expression level in heart was the highest and significantly higher than other tissues at all ages. But, H-FABP mRNA expression level in liver was extreme higher than those in skeletal muscle and adipose tissue at 1 month old age (p<0.01). Besides, H-FABP mRNA expression in adipose tissue appears to increase as the age increase.
  S. Guan , H.L. Zhou , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  Phylogenetic and genetic diversity of four local goat breeds were analyzed by mitochondrial Hypervariable Region (HVR) in 89 individuals from Chinese tropical zone. A total of 36 haplotypes which defined 64 polymorphic sites were found in the study. Comparing with the published mtDNA control region sequences, two mtDNA lineages (A and B) were identified by the phylogenetic analysis in which lineage A was 57.30%, lineage B was 42.70%, haplotype A was 47.22%, haplotype B was 36.11%. The interest in the study was that the proportion of lineage B was up to 80.95% in Hainan black goat, lineage A was only 19.05%. The genetic diversity showed that Hainan black goat had the lowest variability. The average diversity and nucleotide diversity were 0.957±0.007 and 0.02887±0.00256, respectively. A mantel test and the Analysis of Molecular Variance (ANOVA) indicated that there was no significant geographical structuring in Chinese tropical zone goat breeds. Mismatch analysis showed that haplogroup A and B had not experienced population expansion events. According to the study and previous evidence, researchers speculated that lineage B of goat breed might originate from Hainan Island of China.
  B Alipanahi , X Gao , E Karakoc , L Donaldson and M. Li
 

Motivation: Picking peaks from experimental NMR spectra is a key unsolved problem for automated NMR protein structure determination. Such a process is a prerequisite for resonance assignment, nuclear overhauser enhancement (NOE) distance restraint assignment, and structure calculation tasks. Manual or semi-automatic peak picking, which is currently the prominent way used in NMR labs, is tedious, time consuming and costly.

Results: We introduce new ideas, including noise-level estimation, component forming and sub-division, singular value decomposition (SVD)-based peak picking and peak pruning and refinement. PICKY is developed as an automated peak picking method. Different from the previous research on peak picking, we provide a systematic study of the proposed method. PICKY is tested on 32 real 2D and 3D spectra of eight target proteins, and achieves an average of 88% recall and 74% precision. PICKY is efficient. It takes PICKY on average 15.7 s to process an NMR spectrum. More important than these numbers, PICKY actually works in practice. We feed peak lists generated by PICKY to IPASS for resonance assignment, feed IPASS assignment to SPARTA for fragments generation, and feed SPARTA fragments to FALCON for structure calculation. This results in high-resolution structures of several proteins, for example, TM1112, at 1.25 Å.

Availability: PICKY is available upon request. The peak lists of PICKY can be easily loaded by SPARKY to enable a better interactive strategy for rapid peak picking.

Contact: mli@uwaterloo.ca

  H.L. Zhou , S. Guan , G.Y. Hou , D.J. Wang , T.S. Xu , D.F. Wang , L.G. Shi , G. Rong and M. Li
  The genetic diversity and relationships of Hainan Black goat breed among 8 populations were studied using 14 microsatellite markers. In the 14 microsatellite loci, the number of alleles per locus ranged from 15 (BMS1248) to 44 (BMS2258) and the Ho values ranged from 0.003 (BM6404) to 0.923 (BM6444) among the 8 groups, the mean Polymorphic Information Content (PIC) of all the primers detected for all groups showed high values (0.892). For all the goat groups, the mean number of alleles ranged from 9.577 (Baisha) to 16.571 (Saya), the average observed heterozygotes, expected heterozygosity, Polymorphism Information Content (PIC), allelic richness and effective number of alleles was 0.402, 0.866, 0.838, 7.625 and 12.425, respectively. Most of the 14 loci were in Hardy-Weinberg equilibrium (p>0.05) except locus BMS2258 (p<0.0001) and all the 8 populations were in Hardy-Weinberg equilibrium (p>0.05) too. The percentage of variation among the 8 goat groups only amounted to 5.11%, the remaining existed among individuals within populations (58.12%) and within individuals (36.77%). The genetic differentiation pattern and genetic relationships among the 8 populations displayed some degree of consistency with their geographical locations expect Sanya and Linshui regions and suggested that exist a certain inbreeding between SY and CM. The Fis indicated some degree inbreeding existed in Changjiang goat group and there was no significant among the 8 populations. This study will help to interpret the genetic characters of Hainan Black goat and benefit to the future conservation programs.
  M. Li , G. Ji , F. Feng , W. Song , R. Ling , D. Chen , X. Liu , J. Li , H. Shi , W. Wang and H. Zhang
 

Objective: We summarized our experience of living-related small bowel transplantation and postoperative management of 3 patients with short gut syndrome.

Methods: Patient #1, an 18-year-old boy, received a 150-cm segment of distal ileum with a vascular pedicle of distal superior mesenteric artery and vein, which was donated by his father. Patient #2, a 15-year-old boy, received a 160-cm graft of distal ileum from his mother. Patient #3, a 17-year-old boy, received a 170-cm graft of distal ileum from his father. The graft artery and vein were anastomosed to the recipient infrarenal aorta and vena cava, respectively, in end-to-side fashion using 7/0 Prolene suture. Intestinal continuity was restored by anastomosis of proximal end of the graft to the recipients' own proximal jejunum, the distal end was left open as a stoma. The recipient distal gut was anastomosed to the distal end of the graft. All 3 recipients were given FK506 (tacrolimus) regularly combined with periodic mycophenolate mofetil. In cases of acute rejection, large doses of steroids were administered to the recipients.

Results: The recipients and donors had fairly unremarkable postoperative courses. So far, patient #1 has survived for 7 years and 6 months with a well-functioning graft and without requirement for total parenteral nutrition (TPN) support. His body weight increased 20 kg and of his life quality has dramatically improved. Patient #2, however, died of acute rejection with fatal sepsis at 5 months after transplantation. Patient #3 has survived for 3 years and 8 months enjoying a normal life. Postoperative recovery of all 3 donors was unremarkable. They were discharged 12 days after surgery without complications.

Conclusion: Outcomes of the implantation using the distal ileum as a graft in living-related small bowel transplantation have been satisfactory for both recipients and donors. It is feasible to anastomose the graft artery and vein to the recipient infrarenal aorta and vena cava. The intestinal continuity can be restored by a 1-stage strategy with minimal risk to the recipient. Appropriate application and adjustment of immune suppressors are crucial for the recipients to experience high-quality lives.
  W. Li , T. Xu , J.F. Wang , X.F. Wu , M. Li and P.Y. Lu
  Hematopoietic stem cell (HSC) gene transfer has been attempted almost entirely ex vivo and has been limited by loss of self-renewal capacity and transplantation-related defects in homing and engraftment. Herein we have attempted to overcome these limitations by injecting vectors directly into the bone marrow (BM) to transduce HSCs in their native environment. Simian virus 40 (SV40)-derived gene delivery vectors were used because they efficiently transduce resting CD34+ cells. Neonatal C57BL/6 (H-2b) mice (3 days old) received SV(Nef-FLAG), carrying FLAG marker epitope directly into both femoral marrow cavities. Keratinocyte stem cells (KSCs) were purified at 7 and 14 days after SV40 injection. The KSCs from 10-day-old C57BL/6 mice were designated as controls. Flow cytometric (FCM) analyses indicated that KSCs from transgenic mice showed strong down-regulation of surface immunological molecules CD40, CD80, CD86, and human major histocompatibility complex class I chain-related antigen A (MICA). Mixed lymphocyte reaction (MLR) assays showed that transgenic KSCs depressed allogeneic T-cell proliferation. Immunofluorescence showed transgenic KSCs expressed FLAG for the entire study as well as high levels of transforming growth factor (TGF)-β and BCL-2. Thus, direct intramarrow administration of recombinant SV40 yielded efficient gene transfer to mice BM progenitor cells. KSCs with low immunogenicity may be obtained for further investigations of skin transplantation immunity.
 
 
 
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