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Articles by M. Islami
Total Records ( 2 ) for M. Islami
  M. Islami , A. Shabani , M. Saifi-Abolhassan , Sh. Sepehr , M.R. Soudi and S.Z. Mossavi-Nejad
 

Purification and characterization of alcohol dehydrogenase (ADH) from Gluconobacter suboxydans was done in order to biotechnological and industrial application. Solubilization of enzyme from bacterial membrane fraction by Triton X-100 and subsequent fractionation on DEAE-Sephadex A-50 and Hydroxyapatite was successful in enzyme purification. Enzyme assay reaction mixture contained potassium ferricyanide 0.1 M, McIlvaine buffer 0.1 M (pH 5.5), Triton X-100 10%, ethanol 1 M and enzyme solution. The purified ADH Optimum pH activity was 5.5. The enzyme was in maximum stability in pH 5.8. The substrate specificity of the enzyme was determined using the same enzyme assay method as described above, except that various substrates (100 mM) were used instead of ethanol. The relative activity of the ADH for ethanol was higher than the others. The effects of metal ions and inhibitors on the activity of the enzyme were examined by measuring the activity using the same assay method as described above. Activity of purified enzyme was increased in the presence of Ca+2 and was decreased in presence the of ethylenediamine tetra acetic acid (EDTA). Because the proper structure and function of the enzyme is related to structural Ca+2 and EDTA can chelate Ca+2. An apparent Michaelis constant for ethanol were examined to be 1.7x10-3 M for ethanol as substrate.

  E. Rahmatnejad , M. Bojarpour , M. Islami , J. Fayazi and M. Mamouei
  This experiment was conducted to study economic value of Dried Tomato Pomace (DTP) in broiler chickens diets in the form of completely randomized design. For this purpose, 160 days old Ross 308 broilers were distributed into 16 floor pens and reared for 42 days. A basal diet was formulated according to NRC recommendations for starter (0-21 days) and grower (22-42 days) periods. Then, the basal diet was also, supplemented with 8, 16 and 24% DTP, resulting 4 dietary treatments were prepared including control group. The results showed that feed conversion ratio between treatments containing 8 and 16% of DTP compared to control group had no significant difference (p>0.05), but the highest (worst) feed conversion ratio were recorded for birds fed diets supplemented with 24% DTP (p<0.05). Comparison of the cost for 1 kg meat showed that the use of DTP up to 16% in diet reduce production cost.
 
 
 
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