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Articles by M. Hilmi
Total Records ( 5 ) for M. Hilmi
  E. Erwan , A.R. Alimon , A.Q. Sazili , H. Yaakub and M. Hilmi
  Problem statement: Supplementation of broiler diets with cristalline amino acids (i.e. lysine, ethionine and threonine) may support equal broiler growth and improve overall amino acids balance and enable a reduction in CP level of diets. Approach: A trial was conducted to evaluate the effect of supplemental L-leucine in diets containing recommended levels and low crude protein (20 and 18%, respectively) with constant metabolizable energy (3200 kcal kg-1) for broilers from 21-42 day of age. Six experimental diets were formulated with three levels of supplemental L-leucine, 0, 0.5 and 0.67% and two levels of crude protein. A total of 180 1 day-old Cobb broiler chickens were randomly divided into 36 experimental pens, 5 chickens in each pen, with each diet replicated 6 times. The dietary treatments were offered from 21-42 days of age. Feed intake, body weight gain and Feed Conversion Ratio (FCR) were measured on a weekly basis. At the end of the feeding trial the birds were slaughtered and carcass analyses conducted. Results: Feed intake, weight gain and FCR were not affected by increasing levels of L-leucine supplementation. Weight gain was significantly reduced (p<0.05), whereas feed intake and FCR were not significantly affected with decreasing dietary crude protein. A positive response in breast meat yield was achieved by the addition of L-leucine to levels up to 0.5% in the diet but a significant decrease was noted when the level reached 0.67% in diet. Supplementation of L-leucine significantly (p<0.05) decreased the relative weights of the liver and gizzard. However, the addition of L-leucine significantly reduced carcass weights when L-leucine was added at 0.67%. Lowering the dietary protein level also significantly reduced breast yield and carcass weight (p<0.05). However, abdominal fat, gizzard, liver and heart were not affected by protein level. Conclusion/Recommendations: It can be concluded that supplementation of L-leucine at levels up to 0.67% of the diet did not affect performance but deleteratious the carcass weight.
  A.Q. Sazili , M.Z. Noor Azihan , M. Shuhaimi , M. Hilmi and J.M. Panandam
  The present study was conducted in an attempt to study the effects of the type, pH and molarity of extraction buffer on protein extractability from beef Longissimus dorsi (LD), Supraspinatus (SS) and Semitendinosus (ST) muscles. All muscle samples were dissected out from carcass immediately after slaughter and subjected to extractions using freshly prepared buffers of different buffer type (Tris Base and Tris HCl), pH (8.3 and 7.5) and molarity (100 and 20 mM). Following extraction, the total extractable protein concentration was determined by Bradford assay. The results exhibited significant (p<0.05) effects of muscle type X buffer interaction on extracted total protein concentration. The statistical analysis also revealed interaction between type of buffer, pH and molarity significantly (p<0.01), affected the extracted protein concentration. Based on the results, optimal buffer suggested to use for muscle protein extraction is Tris-Base with pH 8.3 and 100 mM. The present study demonstrated that the extractability of skeletal muscle protein was significantly influenced by the type, molarities and pH of the extraction buffers used.
  S. Navid , M. Hilmi , A.Q. Sazili and A. Sheikhlar
  A study was undertaken to improve meat quality of spent layer chicken through supplementing the old layer birds diet with vitamin D3, papaya leaf meal and pineapple skin meal a few days prior to slaughter. Forty spent layers were obtained to conduct this study after a period of laying for 80 weeks. About 4 diets which were fed to the chicken included diet 1: control diet (based on NRC recommendation level to meet or exceed the bird requirements at finisher stage), diet 2: control + (1x105) I.U. Vitamin D3, diet 3: control + 1% Pineapple Skin Meal (PSM) and diet 4: control + 1% Papaya Leaf Meal (PLM). Chicken were kept in an individual cage and fed for 7 days. At day 7, the chicks were scarified, breast muscle was taken for meat color, shear force, pH of the muscle, CP, fat, Ca, drip loss and cooking loss analysis. The results indicated that both vitamin D3 and papaya leaf meal were potent additives to improve tenderness, drip loss, cooking loss and meat color. However, Vitamin D3 appeared to be the most effective one in improving meat quality of spent layers.
  S. Navid , M. Hilmi , A.R. Alimon , A.Q. Sazili and A. Sheikhlar
  Eighty spent chickens were employed in this study to assay the effect of combination of vitamin D3 and papaya leaf on antioxidant activity of meat in spent layer hens. Diets were a corn-soybean meal based diet for finisher layer with and without vitamin D3 which was supplemented with different levels of 0, 0.5, 1 and 2% for papaya leaf meal. Experiment lasted for 21 days. At day 0, 7, 14 and 21, the birds were scarified and breast muscle was obtained to determine antioxidant activity. Antioxidant activity was measured using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH). Result obtained from this study demonstrated that antioxidant activity of meat showed remarkable improvement between dietary treatments fed mix of vitamin D3 and papaya leaf and control group. In conclusion, vitamin D3 and papaya leaf when combined indicated an improvement in antioxidant activity of the spent meat.
  Mamat- Hamidi , I. Idris and M. Hilmi
  Interspecific hybridization has been reported for a wide variety of vertebrate species either spontaneous or by organized crossing of bovine species. The hybrids were often carrying intermediate characters genetically and phenotipically of the parents. Thus, status information of both aspects is valuable in animal production for selection and breeding management. The Gaur-cattle hybrids was reported to be superior in production value compared to their parent cattle but fertility status was still questionable. The project was abandoned due to their fertility issue and the hybrids were kept within the cattle in a dairy farm. Cytogenetic status and breeding record of the remaining herd were unavailable since then. The herd was then translocated to a deer farm (PTH Lenggong) and kept freely in the paddock. Recently, two female calves were born via inter se mating. Peripheral blood cultures of Malayan Gaur, Sahiwal-Friesian cattle and Gaur x cattle hybrid backcrosses were analyzed via Giemsa stained metaphase. The Gaur and cattle were having diploid chromosome number (2n) of 56 and 60, respectively. Interestingly, the backcrosses from the hybrids by cattle bulls were found to have two chromosome arrangements, which are 2n = 58 and 2n = 60.
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