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Articles by M. Ghane
Total Records ( 2 ) for M. Ghane
  M. Eghbali , M. Ghane , M. Mirinargesi and A. Zare Mehrjardi
  Epstein Barr Virus (EBV) infects more than 90% of the world's human population. An association is proposed between EBV infection and occurrence of breast cancer, with a large difference in the results reported by different researchers. This study aimed to investigate the frequency of EBV among benign and malignant breast tumors. A total of 24 carcinomas and 24 fibroadenomas paraffin embedded tumoral tissue samples were obtained from the pathology sections of Toos and Firoozgar hospitals in Tehran, Iran. All samples had been collected from patients from June 2011 to February 2012. DNA was extracted from all samples and their infection with EBV was examined by PCR technique. The results obtained by this study showed that 4 out of 24 carcinoma samples (16.6%) were infected by EBV, while the number of fibroadenoma samples infected by this virus was 1 (4.1%). The frequency of EBV infection was different among malignant and benign tumors. However, no association was observed between EBV infection and the formation of malignant or benign tumors based on the Chi-square test. In relation to some other studies, this analysis does not confirm any association between EBV infection and breast cancer occurrence. However, due to the high frequency of EBV infection among breast cancer patients, future studies are needed to elucidate the possible role of the virus in the disease.
  M. Ghane , B. Yakhchali , M. Khodabandeh and F. Malekzadeh
  In this study, a synthetic gene encoding 166 residues of interferon-β was constructed. For an efficient expression of IFN- β in E. coli, the synthetic gene was designed according to the E. coli codon usage. Cysteine 17 was replaced with serine to avoid multimer formation and random intramolecular disulfide bridges. The sequence of the designed gene was analyzed with Web Cutter and RNA secondary structure softwares to make sure about the cloning and expression of the recombinant gene in the proposed cloning and expression vectors. Twelve overlapping oligonucleotides ranging from 55 to 83 in length were designed. A step-wise assembly polymerase chain reaction was used for the construction of the synthetic gene through three steps PCR programs using overlapping adjacent primers as well as PCR products of a previous steps. The final PCR product was further amplified and cloned into T/A cloning vector. The constructed gene was sub-cloned into pQE30, pKK322 and pGEMEX-1 expression vectors and the expression was analyzed with Western-blotting.
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